Gene description for OBFC1
Gene name oligonucleotide/oligosaccharide-binding fold containing 1
Gene symbol OBFC1
Other names/aliases AAF-44
AAF44
RPA-32
STN1
bA541N10.2
Species Homo sapiens
 Database cross references - OBFC1
ExoCarta ExoCarta_79991
Vesiclepedia VP_79991
Entrez Gene 79991
HGNC 26200
MIM 613128
UniProt Q9H668  
 OBFC1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for OBFC1
Molecular Function
    single-stranded DNA binding GO:0003697 ISS
    protein binding GO:0005515 IPI
    telomeric DNA binding GO:0042162 IBA
    telomeric DNA binding GO:0042162 IDA
    single-stranded telomeric DNA binding GO:0043047 IDA
Biological Process
    telomere maintenance GO:0000723 IMP
    telomere maintenance via telomere lengthening GO:0010833 IBA
    telomere maintenance via telomere lengthening GO:0010833 IMP
    telomere capping GO:0016233 TAS
    negative regulation of telomere maintenance via telomerase GO:0032211 IDA
    positive regulation of DNA replication GO:0045740 ISS
Subcellular Localization
    chromosome, telomeric region GO:0000781 HDA
    chromosome, telomeric region GO:0000781 IDA
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    intermediate filament cytoskeleton GO:0045111 IDA
    CST complex GO:1990879 IBA
    CST complex GO:1990879 IDA
 Experiment description of studies that identified OBFC1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for OBFC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MVP 9961
Two-hybrid Homo sapiens
2 PRIM2 5558
Affinity Capture-MS Homo sapiens
3 CDC34 997
Affinity Capture-MS Homo sapiens
4 ORC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GMNN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CACNG5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 BCKDK 10295
Co-fractionation Homo sapiens
8 MED17  
Affinity Capture-MS Homo sapiens
9 SMC5 23137
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TPP1 1200
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 NDNL2 56160
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 LTBP2 4053
Affinity Capture-MS Homo sapiens
13 MED9  
Affinity Capture-MS Homo sapiens
14 APBA3  
Affinity Capture-MS Homo sapiens
15 CCDC9  
Affinity Capture-MS Homo sapiens
16 MED24 9862
Affinity Capture-MS Homo sapiens
17 FAXC  
Affinity Capture-MS Homo sapiens
18 APP 351
Reconstituted Complex Homo sapiens
19 MED13 9969
Affinity Capture-MS Homo sapiens
20 Lyplal1  
Affinity Capture-MS Mus musculus
21 SPECC1L 23384
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ALPK2  
Affinity Capture-MS Homo sapiens
23 PRIM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MED27  
Affinity Capture-MS Homo sapiens
25 CDK3 1018
Two-hybrid Homo sapiens
26 CTC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FAM136A 84908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 USP33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 KIF3A 11127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MED4 29079
Affinity Capture-MS Homo sapiens
31 MED1 5469
Affinity Capture-MS Homo sapiens
32 ZDHHC17 23390
Two-hybrid Homo sapiens
33 TRIML2  
Two-hybrid Homo sapiens
34 SMC6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 TMEM159 57146
Affinity Capture-MS Homo sapiens
36 TUBGCP4 27229
Two-hybrid Homo sapiens
37 POLA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 LRWD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 WDYHV1  
Two-hybrid Homo sapiens
40 LDLRAP1 26119
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
41 MED25  
Affinity Capture-MS Homo sapiens
42 EPDR1 54749
Affinity Capture-MS Homo sapiens
43 TEN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
44 USP4 7375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 XPO1 7514
Affinity Capture-MS Homo sapiens
46 USP15 9958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 C14orf119  
Two-hybrid Homo sapiens
48 CD6 923
Affinity Capture-MS Homo sapiens
49 TFAP4 7023
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SMCO3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PNMA2  
Affinity Capture-MS Homo sapiens
52 POLR2A 5430
Affinity Capture-MS Homo sapiens
53 UBE2R2 54926
Affinity Capture-MS Homo sapiens
54 FAM120B 84498
Affinity Capture-MS Homo sapiens
55 USP47 55031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 Hnrnpa3 229279
Affinity Capture-MS Mus musculus
57 MED29  
Affinity Capture-MS Homo sapiens
58 MED23 9439
Affinity Capture-MS Homo sapiens
59 RCOR3  
Two-hybrid Homo sapiens
60 ACTR3C 653857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 MED14  
Affinity Capture-MS Homo sapiens
62 MGEA5 10724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 VWC2L  
Affinity Capture-MS Homo sapiens
64 EIF1AD 84285
Affinity Capture-MS Homo sapiens
65 GRN 2896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 NSMCE1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 LDLRAD1  
Affinity Capture-MS Homo sapiens
68 TRIM52  
Affinity Capture-MS Homo sapiens
69 ORC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CNPY2 10330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 MED8  
Affinity Capture-MS Homo sapiens
72 MTMR3  
Affinity Capture-MS Homo sapiens
73 MED6  
Affinity Capture-MS Homo sapiens
74 MKRN3  
Two-hybrid Homo sapiens
75 PRICKLE4  
Affinity Capture-MS Homo sapiens
76 MED30  
Affinity Capture-MS Homo sapiens
77 MED16 10025
Affinity Capture-MS Homo sapiens
78 MED12  
Affinity Capture-MS Homo sapiens
79 BSND  
Affinity Capture-MS Homo sapiens
80 Kif4  
Affinity Capture-MS Mus musculus
81 Cdc37 12539
Affinity Capture-MS Mus musculus
82 C1orf109  
Two-hybrid Homo sapiens
83 HNRNPF 3185
Affinity Capture-MS Homo sapiens
84 POLA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 KCTD17 79734
Affinity Capture-MS Homo sapiens
86 ZNF467  
Affinity Capture-MS Homo sapiens
87 RCOR1  
Affinity Capture-MS Homo sapiens
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