Gene description for FBP1
Gene name fructose-1,6-bisphosphatase 1
Gene symbol FBP1
Other names/aliases FBP
Species Homo sapiens
 Database cross references - FBP1
ExoCarta ExoCarta_2203
Entrez Gene 2203
HGNC 3606
MIM 611570
UniProt P09467  
 FBP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for FBP1
Molecular Function
    protein binding GO:0005515 IPI
    monosaccharide binding GO:0048029 ISS
    metal ion binding GO:0046872 IMP
    AMP binding GO:0016208 IDA
    fructose 1,6-bisphosphate 1-phosphatase activity GO:0042132 IMP
    identical protein binding GO:0042802 IPI
Biological Process
    glucose metabolic process GO:0006006 TAS
    small molecule metabolic process GO:0044281 TAS
    carbohydrate metabolic process GO:0005975 TAS
    dephosphorylation GO:0016311 IMP
    negative regulation of cell growth GO:0030308 IDA
    regulation of gluconeogenesis GO:0006111 IMP
    negative regulation of Ras protein signal transduction GO:0046580 IDA
    cellular response to drug GO:0035690 IDA
    protein homotetramerization GO:0051289 IDA
    fructose 6-phosphate metabolic process GO:0006002 IDA
    fructose metabolic process GO:0006000 TAS
    cellular response to magnesium ion GO:0071286 IDA
    negative regulation of glycolytic process GO:0045820 IDA
    gluconeogenesis GO:0006094 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified FBP1 in exosomes
1
Experiment ID 282
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ASCC2  
Two-hybrid Homo sapiens
2 ATP5J2  
Two-hybrid Homo sapiens
3 ACTN1 87
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
4 C14orf135  
Two-hybrid Homo sapiens
5 FXR2 9513
Two-hybrid Homo sapiens
6 LNX  
Two-hybrid Homo sapiens
7 FBP1 2203
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
8 RNF183  
Two-hybrid Homo sapiens
9 HSPA8 3312
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which FBP1 is involved
PathwayEvidenceSource
Gluconeogenesis TAS Reactome





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