Gene description for SOCS3
Gene name suppressor of cytokine signaling 3
Gene symbol SOCS3
Other names/aliases ATOD4
CIS3
Cish3
SOCS-3
SSI-3
SSI3
Species Homo sapiens
 Database cross references - SOCS3
ExoCarta ExoCarta_9021
Entrez Gene 9021
HGNC 19391
MIM 604176
UniProt O14543  
 SOCS3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for SOCS3
Molecular Function
    protein kinase inhibitor activity GO:0004860 TAS
    protein binding GO:0005515 IPI
Biological Process
    spongiotrophoblast differentiation GO:0060708 IEA
    branching involved in labyrinthine layer morphogenesis GO:0060670 IEA
    negative regulation of protein kinase activity GO:0006469 TAS
    type I interferon signaling pathway GO:0060337 TAS
    regulation of interferon-gamma-mediated signaling pathway GO:0060334 TAS
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    regulation of growth GO:0040008 IEA
    negative regulation of JAK-STAT cascade GO:0046426 IBA
    negative regulation of inflammatory response GO:0050728 IEA
    protein ubiquitination GO:0016567 IEA
    trophoblast giant cell differentiation GO:0060707 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    regulation of type I interferon-mediated signaling pathway GO:0060338 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    positive regulation of cell differentiation GO:0045597 IEA
    placenta blood vessel development GO:0060674 IEA
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    negative regulation of insulin receptor signaling pathway GO:0046627 IBA
    JAK-STAT cascade GO:0007259 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SOCS3 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for SOCS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP3CB  
Invivo Homo sapiens
Invitro Homo sapiens
2 RASA1 5921
Invitro Homo sapiens
Invivo Homo sapiens
3 PRLR  
Affinity Capture-Western Homo sapiens
4 GHR  
Reconstituted Complex Homo sapiens
5 LEPR 3953
Reconstituted Complex Homo sapiens
6 IL6ST 3572
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 PTPN11 5781
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 INSR 3643
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 PIN1 5300
Two-hybrid Homo sapiens
10 JAK2 3717
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 EGFR 1956
Invivo Homo sapiens
Invitro Homo sapiens
12 CSF1R  
Invivo Homo sapiens
13 CXCR4 7852
Affinity Capture-Western Homo sapiens
14 IGF1R 3480
Invivo Homo sapiens
Invitro Homo sapiens
15 EPOR  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here