Gene description for Rpl27
Gene name ribosomal protein L27
Gene symbol Rpl27
Other names/aliases -
Species Mus musculus
 Database cross references - Rpl27
ExoCarta ExoCarta_19942
Vesiclepedia VP_19942
Entrez Gene 19942
UniProt P61358  
 Rpl27 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl27
Molecular Function
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    rRNA processing GO:0006364 ISO
    rRNA processing GO:0006364 ISS
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
    response to aldosterone GO:1904044 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 ISO
    ribosome GO:0005840 NAS
    large ribosomal subunit GO:0015934 ISS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    cytoplasmic side of rough endoplasmic reticulum membrane GO:0098556 ISS
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rpl27 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rpl28 19943
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Elf5  
Affinity Capture-MS Mus musculus
4 Raver1  
Co-fractionation Mus musculus
5 Rpl11 67025
Co-fractionation Mus musculus
6 Rpl13a 22121
Co-fractionation Mus musculus
7 Rpl15 66480
Co-fractionation Mus musculus
8 Rplp2 67186
Co-fractionation Mus musculus
9 Rpl4 67891
Co-fractionation Mus musculus
10 Rpl14 67115
Co-fractionation Mus musculus
11 Rps2 16898
Co-fractionation Mus musculus
12 Rpl10a 19896
Co-fractionation Mus musculus
13 Rps23 66475
Co-fractionation Mus musculus
14 Rpl7a 27176
Co-fractionation Mus musculus
15 Rpl31 114641
Co-fractionation Mus musculus
16 Mapt  
Affinity Capture-MS Mus musculus
17 Rpl6 19988
Co-fractionation Mus musculus
18 Cebpa  
Affinity Capture-MS Mus musculus
19 Rpl24 68193
Co-fractionation Mus musculus
20 Btf3  
Co-fractionation Mus musculus
21 Rps6 20104
Co-fractionation Mus musculus
22 Rpl18a 76808
Co-fractionation Mus musculus
23 Tpt1 22070
Co-fractionation Mus musculus
24 Rpl17 319195
Co-fractionation Mus musculus
25 Rpl22 19934
Co-fractionation Mus musculus
26 Rpl5 100503670
Co-fractionation Mus musculus
27 Fzr1  
Affinity Capture-MS Mus musculus
28 Cyfip1 20430
Affinity Capture-MS Mus musculus
29 Pa2g4 18813
Co-fractionation Mus musculus
30 Rpl7 19989
Co-fractionation Mus musculus
31 Rpl18 19899
Co-fractionation Mus musculus
32 Rps9 76846
Co-fractionation Mus musculus
33 Rpl12 269261
Co-fractionation Mus musculus
34 Gnb2l1 14694
Co-fractionation Mus musculus
35 Rpl35 66489
Co-fractionation Mus musculus
36 Rps3a1 20091
Co-fractionation Mus musculus
37 Copb2 50797
Co-fractionation Mus musculus
38 Rpl36a 19982
Co-fractionation Mus musculus
39 Npm1 18148
Affinity Capture-MS Mus musculus
40 Rpl26 19941
Co-fractionation Mus musculus
41 Rpl13 270106
Co-fractionation Mus musculus
42 Rplp0 11837
Co-fractionation Mus musculus
43 Rpl23a 268449
Co-fractionation Mus musculus
44 Rps14 20044
Co-fractionation Mus musculus
45 Rpl30 19946
Co-fractionation Mus musculus
46 Eed  
Affinity Capture-MS Mus musculus
47 Rpsa 16785
Co-fractionation Mus musculus
48 Rpl8 26961
Co-fractionation Mus musculus
49 Rpl3 27367
Co-fractionation Mus musculus
50 Atg16l1  
Affinity Capture-MS Mus musculus
51 Rpl29 19944
Co-fractionation Mus musculus
52 Fancd2  
Affinity Capture-MS Mus musculus
53 Rars 104458
Co-fractionation Mus musculus
54 Kcna3  
Affinity Capture-MS Mus musculus
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