Gene description for Dhfr
Gene name dihydrofolate reductase
Gene symbol Dhfr
Other names/aliases 8430436I03Rik
AA607882
AI662710
AW555094
Species Mus musculus
 Database cross references - Dhfr
ExoCarta ExoCarta_13361
Vesiclepedia VP_13361
Entrez Gene 13361
UniProt P00375  
 Dhfr identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dhfr
Molecular Function
    mRNA regulatory element binding translation repressor activity GO:0000900 ISO
    mRNA binding GO:0003729 ISO
    mRNA binding GO:0003729 ISS
    dihydrofolate reductase activity GO:0004146 IBA
    dihydrofolate reductase activity GO:0004146 IDA
    dihydrofolate reductase activity GO:0004146 ISO
    dihydrofolate reductase activity GO:0004146 ISS
    folic acid binding GO:0005542 ISO
    NADP binding GO:0050661 IBA
    NADP binding GO:0050661 IEA
    NADP binding GO:0050661 ISO
    dihydrofolic acid binding GO:0051871 IEA
    dihydrofolic acid binding GO:0051871 ISO
    NADPH binding GO:0070402 ISO
    sequence-specific mRNA binding GO:1990825 ISO
Biological Process
    tetrahydrobiopterin biosynthetic process GO:0006729 IMP
    axon regeneration GO:0031103 IEA
    axon regeneration GO:0031103 ISO
    response to methotrexate GO:0031427 IEA
    response to nicotine GO:0035094 IEA
    tetrahydrofolate interconversion GO:0035999 IDA
    dihydrofolate metabolic process GO:0046452 IBA
    dihydrofolate metabolic process GO:0046452 IEA
    dihydrofolate metabolic process GO:0046452 ISO
    tetrahydrofolate metabolic process GO:0046653 ISS
    tetrahydrofolate biosynthetic process GO:0046654 IBA
    tetrahydrofolate biosynthetic process GO:0046654 IEA
    tetrahydrofolate biosynthetic process GO:0046654 ISO
    folic acid metabolic process GO:0046655 IBA
    folic acid metabolic process GO:0046655 IEA
    folic acid metabolic process GO:0046655 ISO
    positive regulation of nitric-oxide synthase activity GO:0051000 IMP
    regulation of removal of superoxide radicals GO:2000121 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial matrix GO:0005759 ISO
    cytosol GO:0005829 TAS
 Experiment description of studies that identified Dhfr in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dhfr
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lrrc59  
Co-fractionation Mus musculus
2 Rpl22 19934
Co-fractionation Mus musculus
3 Rpl7a 27176
Co-fractionation Mus musculus
4 Rps14 20044
Co-fractionation Mus musculus
5 Rpl6 19988
Co-fractionation Mus musculus
6 Gtf2f1  
Co-fractionation Mus musculus
7 Plod3 26433
Co-fractionation Mus musculus
8 Rps6 20104
Co-fractionation Mus musculus
9 Casp6 12368
Co-fractionation Mus musculus
10 Zfp330  
Co-fractionation Mus musculus
11 Rps3a1 20091
Co-fractionation Mus musculus
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