Gene description for Rpl7
Gene name ribosomal protein L7
Gene symbol Rpl7
Other names/aliases Rpl7a
Surf-3
Species Mus musculus
 Database cross references - Rpl7
ExoCarta ExoCarta_19989
Vesiclepedia VP_19989
Entrez Gene 19989
UniProt P14148  
 Rpl7 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl7
Molecular Function
    DNA binding GO:0003677 ISO
    RNA binding GO:0003723 IBA
    mRNA binding GO:0003729 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IC
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    5S rRNA binding GO:0008097 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IBA
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IEA
    cytoplasmic translation GO:0002181 NAS
    rRNA processing GO:0006364 ISO
    translation GO:0006412 IC
    ribosomal large subunit biogenesis GO:0042273 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IDA
    ribosome GO:0005840 ISO
    ribosome GO:0005840 NAS
    postsynaptic density GO:0014069 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    A band GO:0031672 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rpl7 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps7 20115
Co-fractionation Mus musculus
3 Rpl11 67025
Co-fractionation Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Rps18 20084
Co-fractionation Mus musculus
6 Rps3 27050
Co-fractionation Mus musculus
7 Rpl38  
Co-fractionation Mus musculus
8 Rps25 75617
Co-fractionation Mus musculus
9 Rps15a 267019
Co-fractionation Mus musculus
10 Rpl28 19943
Co-fractionation Mus musculus
11 Rpl13a 22121
Co-fractionation Mus musculus
12 Eif5a 276770
Co-fractionation Mus musculus
13 Dhx29  
Co-fractionation Mus musculus
14 Rpsa 16785
Co-fractionation Mus musculus
15 Rplp2 67186
Co-fractionation Mus musculus
16 Gnb2l1 14694
Co-fractionation Mus musculus
17 Rps20 67427
Co-fractionation Mus musculus
18 Eif5a2  
Co-fractionation Mus musculus
19 Rpl14 67115
Co-fractionation Mus musculus
20 Rps2 16898
Co-fractionation Mus musculus
21 Rpl10a 19896
Co-fractionation Mus musculus
22 Rps10 67097
Co-fractionation Mus musculus
23 Rps23 66475
Co-fractionation Mus musculus
24 Rpl7a 27176
Co-fractionation Mus musculus
25 Rpl31 114641
Co-fractionation Mus musculus
26 Rpl27 19942
Co-fractionation Mus musculus
27 Vars 22321
Co-fractionation Mus musculus
28 Rpl32 19951
Co-fractionation Mus musculus
29 Rpl6 19988
Co-fractionation Mus musculus
30 Fzr1  
Affinity Capture-MS Mus musculus
31 Rps19 20085
Co-fractionation Mus musculus
32 Tfe3  
Affinity Capture-MS Mus musculus
33 Rpl24 68193
Co-fractionation Mus musculus
34 Rpl35 66489
Co-fractionation Mus musculus
35 Tpt1 22070
Co-fractionation Mus musculus
36 Rps11 27207
Co-fractionation Mus musculus
37 Rpl18a 76808
Co-fractionation Mus musculus
38 Rps5 20103
Co-fractionation Mus musculus
39 Rpl35a 57808
Co-fractionation Mus musculus
40 Rpl17 319195
Co-fractionation Mus musculus
41 Rpl22 19934
Co-fractionation Mus musculus
42 Rpl5 100503670
Co-fractionation Mus musculus
43 Tmem173  
Proximity Label-MS Mus musculus
44 Eif6 16418
Co-fractionation Mus musculus
45 Rps24 20088
Co-fractionation Mus musculus
46 Rps13 68052
Co-fractionation Mus musculus
47 Rpl12 269261
Co-fractionation Mus musculus
48 Eef1a1 13627
Co-fractionation Mus musculus
49 Rpl21 19933
Co-fractionation Mus musculus
50 Rps21 66481
Co-fractionation Mus musculus
51 Rpl39 67248
Co-fractionation Mus musculus
52 Dmrt2  
Affinity Capture-MS Mus musculus
53 Rplp0 11837
Co-fractionation Mus musculus
54 Rps15 20054
Co-fractionation Mus musculus
55 Rpl18 19899
Co-fractionation Mus musculus
56 Rps3a1 20091
Co-fractionation Mus musculus
57 Rpl36a 19982
Co-fractionation Mus musculus
58 Npm1 18148
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
59 Rpl26 19941
Co-fractionation Mus musculus
60 Rpl13 270106
Co-fractionation Mus musculus
61 Rpl23 65019
Co-fractionation Mus musculus
62 Rpl23a 268449
Co-fractionation Mus musculus
63 Rps14 20044
Co-fractionation Mus musculus
64 Rps17 20068
Co-fractionation Mus musculus
65 Rps9 76846
Co-fractionation Mus musculus
66 Rps6 20104
Co-fractionation Mus musculus
67 Rpl30 19946
Co-fractionation Mus musculus
68 Eed  
Affinity Capture-MS Mus musculus
69 Rpl15 66480
Co-fractionation Mus musculus
70 Eef1b2 55949
Co-fractionation Mus musculus
71 Rpl3 27367
Co-fractionation Mus musculus
72 Tia1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
73 Rps16 20055
Co-fractionation Mus musculus
74 Rpl4 67891
Co-fractionation Mus musculus
75 Fancd2  
Affinity Capture-MS Mus musculus
76 Rps28 54127
Co-fractionation Mus musculus
77 Rpl8 26961
Co-fractionation Mus musculus
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