Gene description for Rpl23
Gene name ribosomal protein L23
Gene symbol Rpl23
Other names/aliases 2810009A01Rik
Species Mus musculus
 Database cross references - Rpl23
ExoCarta ExoCarta_65019
Vesiclepedia VP_65019
Entrez Gene 65019
UniProt P62830  
 Rpl23 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl23
Molecular Function
    transcription coactivator binding GO:0001223 IEA
    transcription coactivator binding GO:0001223 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    large ribosomal subunit rRNA binding GO:0070180 IBA
    ubiquitin ligase inhibitor activity GO:1990948 IEA
    ubiquitin ligase inhibitor activity GO:1990948 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    cytoplasmic translation GO:0002181 NAS
    translation GO:0006412 ISO
    positive regulation of cell population proliferation GO:0008284 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    protein-DNA complex disassembly GO:0032986 IEA
    protein-DNA complex disassembly GO:0032986 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    G1 to G0 transition GO:0070314 IEA
    G1 to G0 transition GO:0070314 ISO
    cellular response to actinomycin D GO:0072717 IEA
    cellular response to actinomycin D GO:0072717 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
    positive regulation of signal transduction by p53 class mediator GO:1901798 IEA
    positive regulation of signal transduction by p53 class mediator GO:1901798 ISO
    regulation of G1 to G0 transition GO:1903450 IEA
    regulation of G1 to G0 transition GO:1903450 ISO
    negative regulation of ubiquitin protein ligase activity GO:1904667 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 IEA
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 ISO
    ribosome GO:0005840 NAS
    postsynaptic density GO:0014069 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    protein-containing complex GO:0032991 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl23 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl23
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rpl28 19943
Co-fractionation Mus musculus
2 Elf5  
Affinity Capture-MS Mus musculus
3 Rpl31 114641
Co-fractionation Mus musculus
4 Mdm2  
Affinity Capture-Western Mus musculus
5 Rpl13a 22121
Co-fractionation Mus musculus
6 Uggt1 320011
Co-fractionation Mus musculus
7 Rpl15 66480
Co-fractionation Mus musculus
8 Rplp2 67186
Co-fractionation Mus musculus
9 Gnb2l1 14694
Co-fractionation Mus musculus
10 Rpl4 67891
Co-fractionation Mus musculus
11 Rpl14 67115
Co-fractionation Mus musculus
12 Rps2 16898
Co-fractionation Mus musculus
13 Rpl10a 19896
Co-fractionation Mus musculus
14 Rps8 20116
Co-fractionation Mus musculus
15 Tfe3  
Affinity Capture-MS Mus musculus
16 Rpl7a 27176
Co-fractionation Mus musculus
17 Snd1 56463
Co-fractionation Mus musculus
18 Rpl11 67025
Co-fractionation Mus musculus
19 Atxn1  
Proximity Label-MS Mus musculus
20 Rpl6 19988
Co-fractionation Mus musculus
21 Kctd13  
Affinity Capture-MS Mus musculus
22 Eef1g 67160
Co-fractionation Mus musculus
23 Pkm 18746
Co-fractionation Mus musculus
24 Rpl24 68193
Co-fractionation Mus musculus
25 Rps6 20104
Co-fractionation Mus musculus
26 Rpl18a 76808
Co-fractionation Mus musculus
27 Rpl8 26961
Co-fractionation Mus musculus
28 Rpl17 319195
Co-fractionation Mus musculus
29 Rpl22 19934
Co-fractionation Mus musculus
30 Mettl21e  
Affinity Capture-MS Mus musculus
31 Rps16 20055
Co-fractionation Mus musculus
32 Tmem173  
Proximity Label-MS Mus musculus
33 Fzr1  
Affinity Capture-MS Mus musculus
34 Eef2 13629
Co-fractionation Mus musculus
35 Rps11 27207
Co-fractionation Mus musculus
36 Raver1  
Co-fractionation Mus musculus
37 Pa2g4 18813
Co-fractionation Mus musculus
38 Rpl7 19989
Co-fractionation Mus musculus
39 Eef1a1 13627
Co-fractionation Mus musculus
40 Rpl21 19933
Co-fractionation Mus musculus
41 Rps21 66481
Co-fractionation Mus musculus
42 Rpl12 269261
Co-fractionation Mus musculus
43 Rpl38  
Co-fractionation Mus musculus
44 Rpl18 19899
Co-fractionation Mus musculus
45 Rps3a1 20091
Co-fractionation Mus musculus
46 Copb2 50797
Co-fractionation Mus musculus
47 Pde12  
Co-fractionation Mus musculus
48 Rpl36a 19982
Co-fractionation Mus musculus
49 Rpl26 19941
Co-fractionation Mus musculus
50 Fancd2  
Affinity Capture-MS Mus musculus
51 Rplp0 11837
Co-fractionation Mus musculus
52 Rpl23a 268449
Co-fractionation Mus musculus
53 Rps14 20044
Co-fractionation Mus musculus
54 Eed  
Affinity Capture-MS Mus musculus
55 Rpsa 16785
Co-fractionation Mus musculus
56 AKT1 207
Two-hybrid Homo sapiens
57 Rpl3 27367
Co-fractionation Mus musculus
58 Rpl5 100503670
Co-fractionation Mus musculus
59 Rpl13 270106
Co-fractionation Mus musculus
60 Kcna3  
Affinity Capture-MS Mus musculus
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