Gene description for NET1
Gene name neuroepithelial cell transforming 1
Gene symbol NET1
Other names/aliases ARHGEF8
NET1A
Species Homo sapiens
 Database cross references - NET1
ExoCarta ExoCarta_10276
Vesiclepedia VP_10276
Entrez Gene 10276
HGNC 14592
MIM 606450
UniProt Q7Z628  
 NET1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for NET1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
Biological Process
    regulation of cell growth GO:0001558 NAS
    signal transduction GO:0007165 TAS
    positive regulation of Rho protein signal transduction GO:0035025 IBA
    intracellular signal transduction GO:0035556 IEA
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of GTPase activity GO:0043547 IMP
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    myoblast migration GO:0051451 IEA
    cellular response to hydrogen peroxide GO:0070301 IDA
    cellular response to ionizing radiation GO:0071479 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NET1 in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NET1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LBR 3930
Affinity Capture-MS Homo sapiens
2 MYL6B 140465
Affinity Capture-MS Homo sapiens
3 Magi1  
Protein-peptide Mus musculus
4 RPS27A 6233
Affinity Capture-MS Homo sapiens
5 SNX27 81609
Affinity Capture-MS Homo sapiens
6 Lin7c 22343
Protein-peptide Mus musculus
7 Mpp6 56524
Protein-peptide Mus musculus
8 RACGAP1 29127
Affinity Capture-MS Homo sapiens
9 NCAPH2  
Affinity Capture-MS Homo sapiens
10 Dlg3  
Reconstituted Complex Mus musculus
Protein-peptide Mus musculus
11 KPNB1 3837
Affinity Capture-MS Homo sapiens
12 DLG1 1739
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
13 Dlg4  
Reconstituted Complex Mus musculus
Protein-peptide Mus musculus
14 USP11 8237
Affinity Capture-MS Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 CALM1 801
Affinity Capture-MS Homo sapiens
17 Dlg1 13383
Reconstituted Complex Mus musculus
Protein-peptide Mus musculus
18 TAF1  
Affinity Capture-MS Homo sapiens
19 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
20 IL4R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RBFOX2 23543
Affinity Capture-MS Homo sapiens
22 Mpp2  
Protein-peptide Mus musculus
23 MAGI3 260425
Affinity Capture-MS Homo sapiens
24 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 DCAF7 10238
Affinity Capture-MS Homo sapiens
26 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 Cask 12361
Protein-peptide Mus musculus
28 APBA1  
Affinity Capture-MS Homo sapiens
29 ABCD3 5825
Affinity Capture-MS Homo sapiens
30 PRKAB2 5565
Affinity Capture-MS Homo sapiens
31 HEATR1 55127
Affinity Capture-MS Homo sapiens
32 RHOA 387
Two-hybrid Homo sapiens
FRET Homo sapiens
33 MAGI1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
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