Gene description for Cct3
Gene name chaperonin containing Tcp1, subunit 3 (gamma)
Gene symbol Cct3
Other names/aliases AL024092
Cctg
Tcp1-rs3
TriC-P5
Species Mus musculus
 Database cross references - Cct3
ExoCarta ExoCarta_12462
Vesiclepedia VP_12462
Entrez Gene 12462
UniProt P80318  
 Cct3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cct3
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    protein folding chaperone GO:0044183 ISO
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IBA
    protein folding GO:0006457 ISO
    binding of sperm to zona pellucida GO:0007339 IDA
    positive regulation of telomere maintenance via telomerase GO:0032212 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    chaperone mediated protein folding independent of cofactor GO:0051086 IEA
    chaperone-mediated protein folding GO:0061077 ISO
    positive regulation of establishment of protein localization to telomere GO:1904851 ISO
Subcellular Localization
    zona pellucida receptor complex GO:0002199 IDA
    chaperonin-containing T-complex GO:0005832 IBA
    chaperonin-containing T-complex GO:0005832 IDA
    chaperonin-containing T-complex GO:0005832 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    myelin sheath GO:0043209 HDA
    cell body GO:0044297 IDA
 Experiment description of studies that identified Cct3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
8
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cct3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Affinity Capture-MS Homo sapiens
2 AIDA 64853
Affinity Capture-MS Homo sapiens
3 TUBB2A 7280
Affinity Capture-MS Homo sapiens
4 ACTR2 10097
Affinity Capture-MS Homo sapiens
5 CCT7 10574
Affinity Capture-MS Homo sapiens
6 Wdr82  
Co-fractionation Mus musculus
7 Mycbp2 105689
Affinity Capture-MS Mus musculus
8 PDCD6 10016
Affinity Capture-MS Homo sapiens
9 TUBB 203068
Affinity Capture-MS Homo sapiens
10 CCT8 10694
Affinity Capture-MS Homo sapiens
11 HDAC2 3066
Affinity Capture-MS Homo sapiens
12 PDCL3 79031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 WDR77 79084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 Pou5f1  
Affinity Capture-MS Mus musculus
15 TRMT10C 54931
Affinity Capture-MS Homo sapiens
16 XPO1 7514
Affinity Capture-MS Homo sapiens
17 TNPO1 3842
Affinity Capture-MS Homo sapiens
18 PDCL 5082
Affinity Capture-MS Homo sapiens
19 CCT6A 908
Affinity Capture-MS Homo sapiens
20 MLST8 64223
Affinity Capture-MS Homo sapiens
21 Tsc1  
Affinity Capture-MS Mus musculus
22 Vars 22321
Co-fractionation Mus musculus
23 Atxn1  
Proximity Label-MS Mus musculus
24 Kcna3  
Affinity Capture-MS Mus musculus
25 Kctd13  
Affinity Capture-MS Mus musculus
26 PFDN1 5201
Affinity Capture-MS Homo sapiens
27 SLC35E1 79939
Affinity Capture-MS Homo sapiens
28 RPUSD4 84881
Affinity Capture-MS Homo sapiens
29 Tfe3  
Affinity Capture-MS Mus musculus
30 TLE3  
Affinity Capture-MS Homo sapiens
31 CCT4 10575
Affinity Capture-MS Homo sapiens
32 CCT2 10576
Affinity Capture-MS Homo sapiens
33 SLC35F6 54978
Affinity Capture-MS Homo sapiens
34 HGH1 51236
Affinity Capture-MS Homo sapiens
35 Iqcb1  
Affinity Capture-MS Mus musculus
36 PPL 5493
Affinity Capture-MS Homo sapiens
37 GFM2 84340
Affinity Capture-MS Homo sapiens
38 Cct2 12461
Co-fractionation Mus musculus
39 TXNDC9 10190
Affinity Capture-MS Homo sapiens
40 CCT5 22948
Affinity Capture-MS Homo sapiens
41 Mettl21e  
Affinity Capture-MS Mus musculus
42 Tmem173  
Proximity Label-MS Mus musculus
43 PFDN2 5202
Affinity Capture-MS Homo sapiens
44 HACD2 201562
Affinity Capture-MS Homo sapiens
45 Cyfip1 20430
Affinity Capture-MS Mus musculus
46 Ppp2r2a 71978
Co-fractionation Mus musculus
47 PDCD5 9141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 Trim28 21849
Co-fractionation Mus musculus
49 TUBA1B 10376
Affinity Capture-MS Homo sapiens
50 Atg5  
Affinity Capture-RNA Mus musculus
51 GNB2 2783
Affinity Capture-MS Homo sapiens
52 ACTL8  
Affinity Capture-MS Homo sapiens
53 Strn3  
Co-fractionation Mus musculus
54 EEF2 1938
Affinity Capture-MS Homo sapiens
55 FMN1 342184
Affinity Capture-MS Homo sapiens
56 WDR92 116143
Affinity Capture-MS Homo sapiens
57 Eed  
Affinity Capture-MS Mus musculus
58 Ubash3b  
Co-fractionation Mus musculus
59 Mapt  
Affinity Capture-MS Mus musculus
60 Atg16l1  
Affinity Capture-MS Mus musculus
61 Ppp2r2d  
Co-fractionation Mus musculus
62 Fancd2  
Affinity Capture-MS Mus musculus
63 TCP1 6950
Affinity Capture-MS Homo sapiens
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