Gene description for OTUD7B
Gene name OTU deubiquitinase 7B
Gene symbol OTUD7B
Other names/aliases CEZANNE
ZA20D1
Species Homo sapiens
 Database cross references - OTUD7B
ExoCarta ExoCarta_56957
Vesiclepedia VP_56957
Entrez Gene 56957
HGNC 16683
MIM 611748
UniProt Q6GQQ9  
 OTUD7B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for OTUD7B
Molecular Function
    DNA binding GO:0003677 IEA
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 ISS
    protein binding GO:0005515 IPI
    cysteine-type peptidase activity GO:0008234 ISS
    zinc ion binding GO:0008270 IEA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IBA
    K48-linked deubiquitinase activity GO:1990380 IDA
    K48-linked deubiquitinase activity GO:1990380 TAS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    in utero embryonic development GO:0001701 IEA
    adaptive immune response GO:0002250 IEA
    mucosal immune response GO:0002385 ISS
    protein deubiquitination GO:0016579 TAS
    negative regulation of interleukin-8 production GO:0032717 IDA
    protein K11-linked deubiquitination GO:0035871 IDA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IBA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IDA
    protein K63-linked deubiquitination GO:0070536 IDA
    protein K48-linked deubiquitination GO:0071108 IDA
    protein K48-linked deubiquitination GO:0071108 ISS
    protein deubiquitination involved in ubiquitin-dependent protein catabolic process GO:0071947 IBA
    negative regulation of protein localization to nucleus GO:1900181 IDA
    negative regulation of TORC1 signaling GO:1904262 IDA
    positive regulation of TORC2 signaling GO:1904515 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified OTUD7B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for OTUD7B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MTSS1L 92154
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 OTUD7A 161725
Affinity Capture-MS Homo sapiens
4 UBE2S  
Biochemical Activity Homo sapiens
5 MAP3K14  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 EPM2AIP1 9852
Two-hybrid Homo sapiens
7 LGR4 55366
Affinity Capture-MS Homo sapiens
8 TAX1BP1 8887
Affinity Capture-Western Homo sapiens
9 XPO1 7514
Co-localization Homo sapiens
10 AGO1  
Affinity Capture-RNA Homo sapiens
11 WWOX 51741
Affinity Capture-MS Homo sapiens
12 NUP155 9631
Affinity Capture-MS Homo sapiens
13 EPAS1  
Affinity Capture-Western Homo sapiens
14 AURKA 6790
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
15 ZDHHC17 23390
Two-hybrid Homo sapiens
16 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
17 TRAF6 7189
Affinity Capture-Western Homo sapiens
18 CUL3 8452
Affinity Capture-MS Homo sapiens
19 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
20 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
22 FAM168A  
Affinity Capture-MS Homo sapiens
23 MAVS 57506
Affinity Capture-Western Homo sapiens
24 PARK7 11315
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 MSH3 4437
Co-fractionation Homo sapiens
26 TRAF3 7187
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 HSFY1  
Two-hybrid Homo sapiens
28 FZR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 DAZAP2  
Two-hybrid Homo sapiens
30 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CCNB1 891
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
33 BECN1 8678
Affinity Capture-Western Homo sapiens
34 MYC  
Affinity Capture-Western Homo sapiens
35 YAP1 10413
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 ME1 4199
Affinity Capture-MS Homo sapiens
37 EGFR 1956
Affinity Capture-Western Homo sapiens
38 HSFY2  
Two-hybrid Homo sapiens
39 CDC20 991
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 SPG20 23111
Two-hybrid Homo sapiens
41 FOXM1  
Affinity Capture-Western Homo sapiens
42 ACAD9 28976
Affinity Capture-MS Homo sapiens
43 ULK1  
Affinity Capture-Western Homo sapiens
44 OTULIN 90268
Two-hybrid Homo sapiens
45 PPP1R32  
Two-hybrid Homo sapiens
46 MLST8 64223
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
47 PIK3C3 5289
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
48 IRF3 3661
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 HIF1A 3091
Affinity Capture-Western Homo sapiens
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