Gene description for BCHE
Gene name butyrylcholinesterase
Gene symbol BCHE
Other names/aliases CHE1
CHE2
E1
Species Homo sapiens
 Database cross references - BCHE
ExoCarta ExoCarta_590
Vesiclepedia VP_590
Entrez Gene 590
HGNC 983
MIM 177400
UniProt P06276  
 BCHE identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Plasma 36624553    
Plasma 36624553    
Prostate cancer cells 22723089    
 Gene ontology annotations for BCHE
Molecular Function
    amyloid-beta binding GO:0001540 NAS
    catalytic activity GO:0003824 NAS
    acetylcholinesterase activity GO:0003990 IBA
    acetylcholinesterase activity GO:0003990 ISS
    cholinesterase activity GO:0004104 IDA
    protein binding GO:0005515 IPI
    hydrolase activity, acting on ester bonds GO:0016788 TAS
    enzyme binding GO:0019899 NAS
    choline binding GO:0033265 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    acetylcholine catabolic process GO:0006581 IBA
    xenobiotic metabolic process GO:0006805 TAS
    learning GO:0007612 IEA
    negative regulation of cell population proliferation GO:0008285 IEA
    neuroblast differentiation GO:0014016 IEA
    peptide hormone processing GO:0016486 TAS
    choline metabolic process GO:0019695 IBA
    response to alkaloid GO:0043279 IEA
    cocaine metabolic process GO:0050783 TAS
    negative regulation of synaptic transmission GO:0050805 IEA
    response to glucocorticoid GO:0051384 IEA
    response to folic acid GO:0051593 IEA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    nuclear envelope lumen GO:0005641 IEA
    endoplasmic reticulum lumen GO:0005788 IEA
    plasma membrane GO:0005886 IBA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified BCHE in exosomes
1
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 606
MISEV standards
EM
Biophysical techniques
CD9|HSP90|TSG101|GAPDH
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 606
MISEV standards
EM
Biophysical techniques
CD9|HSP90|TSG101|GAPDH
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 607
MISEV standards
EM
Biophysical techniques
CD9|HSP90|TSG101|GAPDH
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 607
MISEV standards
EM
Biophysical techniques
CD9|HSP90|TSG101|GAPDH
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 143
MISEV standards
EM
Biophysical techniques
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
 Protein-protein interactions for BCHE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBA3C 7278
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CCL3  
Affinity Capture-MS Homo sapiens
3 LY6H  
Affinity Capture-MS Homo sapiens
4 CST5 1473
Affinity Capture-MS Homo sapiens
5 NHP2 55651
Affinity Capture-MS Homo sapiens
6 GPIHBP1  
Affinity Capture-MS Homo sapiens
7 LOXL1 4016
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 Sgol2  
Affinity Capture-MS Mus musculus
9 CCDC6 8030
Affinity Capture-MS Homo sapiens
10 UGGT2 55757
Affinity Capture-MS Homo sapiens
11 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
12 SLURP1  
Affinity Capture-MS Homo sapiens
13 CD14 929
Affinity Capture-MS Homo sapiens
14 FAM120A 23196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LCN6  
Affinity Capture-MS Homo sapiens
17 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 NDP  
Affinity Capture-MS Homo sapiens
19 DEFB109P1B  
Affinity Capture-MS Homo sapiens
20 ENTPD5 957
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SCGB1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MANEAL 149175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PDGFRA 5156
Affinity Capture-MS Homo sapiens
24 SF1 7536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RLN1  
Affinity Capture-MS Homo sapiens
26 PALLD 23022
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ASPH 444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 GRIN2C 2905
Affinity Capture-MS Homo sapiens
29 LOC254896  
Affinity Capture-MS Homo sapiens
30 TRIM55  
Two-hybrid Homo sapiens
31 FBXO6 26270
Affinity Capture-MS Homo sapiens
32 SCGB1C1  
Affinity Capture-MS Homo sapiens
33 COLQ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
34 SLAMF1 6504
Affinity Capture-MS Homo sapiens
35 KIAA1598 57698
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LTBP1 4052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ISM2  
Affinity Capture-MS Homo sapiens
38 MAU2  
Affinity Capture-MS Homo sapiens
39 DKC1 1736
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 GKN2  
Affinity Capture-MS Homo sapiens
41 SEC11A 23478
Two-hybrid Homo sapiens
42 FBXO2 26232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ACTN3 89
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SCGB2A2  
Affinity Capture-MS Homo sapiens
45 C1orf54  
Affinity Capture-MS Homo sapiens
46 PSCA 8000
Affinity Capture-MS Homo sapiens
47 BCHE 590
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
48 SMR3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 DIAPH3 81624
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 HYOU1 10525
Affinity Capture-MS Homo sapiens
52 CPSF7 79869
Affinity Capture-MS Homo sapiens
53 ST14 6768
Affinity Capture-MS Homo sapiens
54 NAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CANX 821
Affinity Capture-MS Homo sapiens
56 CTAGE5 4253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 NOP10 55505
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ENAH 55740
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 C1QTNF7  
Affinity Capture-MS Homo sapiens
60 TRIM63  
Two-hybrid Homo sapiens
61 NMS  
Affinity Capture-MS Homo sapiens
62 WASL 8976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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