Gene description for ABCA2
Gene name ATP-binding cassette, sub-family A (ABC1), member 2
Gene symbol ABCA2
Other names/aliases ABC2
Species Homo sapiens
 Database cross references - ABCA2
ExoCarta ExoCarta_20
Vesiclepedia VP_20
Entrez Gene 20
HGNC 32
MIM 600047
UniProt Q9BZC7  
 ABCA2 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ABCA2
Molecular Function
    nucleotide binding GO:0000166 NAS
    lipid transporter activity GO:0005319 IBA
    ATP binding GO:0005524 IDA
    ATP binding GO:0005524 ISS
    ATP hydrolysis activity GO:0016887 IEA
    ATPase-coupled transmembrane transporter activity GO:0042626 IBA
    ATPase-coupled transmembrane transporter activity GO:0042626 NAS
    endopeptidase regulator activity GO:0061135 IMP
    ceramide floppase activity GO:0099038 IDA
    ABC-type transporter activity GO:0140359 IEA
Biological Process
    ganglioside metabolic process GO:0001573 ISS
    lipid metabolic process GO:0006629 NAS
    sphingomyelin metabolic process GO:0006684 ISS
    glycosphingolipid metabolic process GO:0006687 ISS
    lipid transport GO:0006869 IBA
    locomotory behavior GO:0007626 ISS
    response to xenobiotic stimulus GO:0009410 TAS
    regulation of steroid metabolic process GO:0019218 IDA
    central nervous system myelin formation GO:0032289 ISS
    central nervous system myelin formation GO:0032289 ISS
    regulation of intracellular cholesterol transport GO:0032383 IMP
    regulation of intracellular cholesterol transport GO:0032383 ISS
    negative regulation of intracellular cholesterol transport GO:0032384 IDA
    positive regulation of low-density lipoprotein particle receptor catabolic process GO:0032805 IDA
    cholesterol homeostasis GO:0042632 IEP
    positive regulation of amyloid precursor protein biosynthetic process GO:0042986 IDA
    regulation of cholesterol biosynthetic process GO:0045540 IDA
    negative regulation of steroid metabolic process GO:0045939 ISS
    sphingosine biosynthetic process GO:0046512 IDA
    response to steroid hormone GO:0048545 IEP
    transmembrane transport GO:0055085 NAS
    regulation of protein glycosylation GO:0060049 ISS
    response to cholesterol GO:0070723 IEP
    negative regulation of phospholipid biosynthetic process GO:0071072 IDA
    negative regulation of sphingolipid biosynthetic process GO:0090155 IDA
    intracellular sphingolipid homeostasis GO:0090156 IDA
    negative regulation of cholesterol efflux GO:0090370 IDA
    ceramide translocation GO:0099040 IDA
    transport across blood-brain barrier GO:0150104 NAS
    regulation of post-translational protein modification GO:1901873 IMP
    positive regulation of amyloid-beta formation GO:1902004 IMP
    positive regulation of amyloid-beta formation GO:1902004 ISS
    positive regulation of amyloid precursor protein catabolic process GO:1902993 IGI
    positive regulation of amyloid precursor protein catabolic process GO:1902993 IMP
    regulation of protein localization to cell periphery GO:1904375 ISS
    negative regulation of receptor-mediated endocytosis involved in cholesterol transport GO:1905601 IDA
    regulation of protein localization to cell surface GO:2000008 ISS
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 ISS
    lysosomal membrane GO:0005765 NAS
    endosome GO:0005768 IDA
    microtubule organizing center GO:0005815 ISS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    endosome membrane GO:0010008 IDA
    endosome membrane GO:0010008 IEA
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    membrane GO:0016020 NAS
    cytoplasmic vesicle GO:0031410 ISS
    ATP-binding cassette (ABC) transporter complex GO:0043190 NAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified ABCA2 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ABCA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HFE  
Affinity Capture-MS Homo sapiens
2 RNASEH2B  
Affinity Capture-MS Homo sapiens
3 LCN6  
Affinity Capture-MS Homo sapiens
4 LGALS1 3956
Affinity Capture-MS Homo sapiens
5 TMEM9B 56674
Affinity Capture-MS Homo sapiens
6 SLC22A9  
Affinity Capture-MS Homo sapiens
7 ETNK1  
Affinity Capture-MS Homo sapiens
8 FGFR3 2261
Affinity Capture-MS Homo sapiens
9 ABCA6 23460
Affinity Capture-MS Homo sapiens
10 OR10H1  
Affinity Capture-MS Homo sapiens
11 HLA-B 3106
Affinity Capture-MS Homo sapiens
12 NT5C3A 51251
Affinity Capture-MS Homo sapiens
13 PIGH  
Affinity Capture-MS Homo sapiens
14 ATP2A3 489
Affinity Capture-MS Homo sapiens
15 C3orf52  
Affinity Capture-MS Homo sapiens
16 YWHAH 7533
Affinity Capture-MS Homo sapiens
17 PMS2  
Affinity Capture-MS Homo sapiens
18 XIRP1  
Affinity Capture-MS Homo sapiens
19 LGALS9 3965
Affinity Capture-MS Homo sapiens
20 Ankfy1 11736
Affinity Capture-MS Mus musculus
21 CLGN 1047
Affinity Capture-MS Homo sapiens
22 KIF14 9928
Affinity Capture-MS Homo sapiens
23 TMEM248 55069
Affinity Capture-MS Homo sapiens
24 ATP5A1 498
Affinity Capture-MS Homo sapiens
25 P2RY8  
Affinity Capture-MS Homo sapiens
26 OR4N2  
Affinity Capture-MS Homo sapiens
27 PIGM  
Affinity Capture-MS Homo sapiens
28 SARAF  
Affinity Capture-MS Homo sapiens
29 ST7L  
Affinity Capture-MS Homo sapiens
30 C1orf54  
Affinity Capture-MS Homo sapiens
31 GPR182  
Affinity Capture-MS Homo sapiens
32 ITM2C 81618
Affinity Capture-MS Homo sapiens
33 SLC12A4 6560
Affinity Capture-MS Homo sapiens
34 ABCB9  
Affinity Capture-MS Homo sapiens
35 SDF2L1 23753
Affinity Capture-MS Homo sapiens
36 CRISP1  
Affinity Capture-MS Homo sapiens
37 ATP5B 506
Affinity Capture-MS Homo sapiens
38 UPK2 7379
Affinity Capture-MS Homo sapiens
39 C7orf34  
Affinity Capture-MS Homo sapiens
40 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 FBXO45 200933
Affinity Capture-MS Homo sapiens
42 FAM189B  
Affinity Capture-MS Homo sapiens
43 CACNA1A  
Two-hybrid Homo sapiens
44 TTYH1  
Affinity Capture-MS Homo sapiens
45 MYCBP2 23077
Affinity Capture-MS Homo sapiens
46 CANX 821
Affinity Capture-MS Homo sapiens
47 PCDH1 5097
Affinity Capture-MS Homo sapiens
48 ZDHHC12  
Affinity Capture-MS Homo sapiens
49 PON2 5445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ATP5F1 515
Affinity Capture-MS Homo sapiens
51 KLRD1  
Affinity Capture-MS Homo sapiens
52 GUCY1B3  
Affinity Capture-MS Homo sapiens
53 SNX14  
Affinity Capture-MS Homo sapiens
54 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 OCIAD1 54940
Affinity Capture-MS Homo sapiens
56 NXF1 10482
Affinity Capture-RNA Homo sapiens
57 CDK5RAP2 55755
Two-hybrid Homo sapiens
58 KCNE3  
Affinity Capture-MS Homo sapiens
59 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
60 DNAJC18  
Affinity Capture-MS Homo sapiens
61 MYC  
Affinity Capture-MS Homo sapiens
62 FBXO2 26232
Affinity Capture-MS Homo sapiens
63 GPR45  
Affinity Capture-MS Homo sapiens
64 ITPRIPL1  
Affinity Capture-MS Homo sapiens
65 TGFA  
Affinity Capture-MS Homo sapiens
66 GPATCH8  
Affinity Capture-MS Homo sapiens
67 CLTC 1213
Affinity Capture-MS Homo sapiens
68 DRD2  
Two-hybrid Homo sapiens
69 SDHAF2 54949
Affinity Capture-MS Homo sapiens
70 ADCY3 109
Affinity Capture-MS Homo sapiens
71 PEX11B 8799
Affinity Capture-MS Homo sapiens
72 BTNL3  
Affinity Capture-MS Homo sapiens
73 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
74 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
75 CLEC2D  
Affinity Capture-MS Homo sapiens
76 TSPAN15 23555
Affinity Capture-MS Homo sapiens
77 LMAN2L 81562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 MRAP2  
Affinity Capture-MS Homo sapiens
79 RAB32 10981
Affinity Capture-MS Homo sapiens
80 EDEM2  
Affinity Capture-MS Homo sapiens
81 HTR6  
Two-hybrid Homo sapiens
82 Myh10 77579
Affinity Capture-MS Mus musculus
83 SFTPC  
Affinity Capture-MS Homo sapiens
84 PDZD8 118987
Affinity Capture-MS Homo sapiens
85 ZNRF2 223082
Affinity Capture-MS Homo sapiens
86 BRICD5  
Affinity Capture-MS Homo sapiens
87 FAM69A 388650
Affinity Capture-MS Homo sapiens
88 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 LAMB3 3914
Affinity Capture-MS Homo sapiens
90 VAMP5 10791
Affinity Capture-MS Homo sapiens
91 MARCH4  
Affinity Capture-MS Homo sapiens
92 ATP5C1 509
Affinity Capture-MS Homo sapiens
93 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
94 PCDHB3  
Affinity Capture-MS Homo sapiens
95 ABCD4 5826
Affinity Capture-MS Homo sapiens
96 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 CLEC4E  
Affinity Capture-MS Homo sapiens
98 HLA-C 3107
Affinity Capture-MS Homo sapiens
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