Gene description for HOMER3
Gene name homer scaffolding protein 3
Gene symbol HOMER3
Other names/aliases HOMER-3
VESL3
Species Homo sapiens
 Database cross references - HOMER3
ExoCarta ExoCarta_9454
Vesiclepedia VP_9454
Entrez Gene 9454
HGNC 17514
MIM 604800
UniProt Q9NSC5  
 HOMER3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HOMER3
Molecular Function
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IEA
    G protein-coupled glutamate receptor binding GO:0035256 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    protein targeting GO:0006605 TAS
    G protein-coupled glutamate receptor signaling pathway GO:0007216 IBA
    negative regulation of interleukin-2 production GO:0032703 IMP
    negative regulation of calcineurin-NFAT signaling cascade GO:0070885 IMP
    regulation of store-operated calcium entry GO:2001256 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    postsynaptic density GO:0014069 IBA
    dendrite GO:0030425 IBA
    basal part of cell GO:0045178 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified HOMER3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HOMER3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 EFHC1  
Two-hybrid Homo sapiens
3 POLG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NEBL 10529
Two-hybrid Homo sapiens
5 INADL 10207
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HOMER3 9454
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
8 USP2  
Two-hybrid Homo sapiens
9 NUP54 53371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 WBP2 23558
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
11 DSN1 79980
Negative Genetic Homo sapiens
12 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
13 CEP192 55125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CYFIP2 26999
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 MPP5 64398
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TP53 7157
Affinity Capture-MS Homo sapiens
17 WIPF1 7456
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 Bcas2  
Affinity Capture-MS Mus musculus
19 NCKAP1 10787
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 MYPN 84665
Affinity Capture-MS Homo sapiens
21 NHLRC2 374354
Affinity Capture-MS Homo sapiens
22 DBN1 1627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HNRNPA2B1 3181
Negative Genetic Homo sapiens
24 PALLD 23022
Affinity Capture-MS Homo sapiens
25 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NR3C1 2908
Proximity Label-MS Homo sapiens
27 SOX17  
Affinity Capture-MS Homo sapiens
28 PSMA2 5683
Two-hybrid Homo sapiens
29 SPOPL  
Affinity Capture-MS Homo sapiens
30 SRPK2 6733
Biochemical Activity Homo sapiens
31 MOS 4342
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 Cep44  
Affinity Capture-MS Mus musculus
33 Csk 12988
Affinity Capture-MS Mus musculus
34 HOMER1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 EIF4E2  
Affinity Capture-MS Homo sapiens
36 TRPC4  
Affinity Capture-Western Homo sapiens
37 APP 351
Reconstituted Complex Homo sapiens
38 FRYL 285527
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PSMA1 5682
Two-hybrid Homo sapiens
40 Cdc16  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
41 TRPC5  
Affinity Capture-Western Homo sapiens
42 APEX1 328
Affinity Capture-RNA Homo sapiens
43 RYR1 6261
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
44 RUNX1T1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 CEBPA  
Protein-peptide Homo sapiens
46 ABI2 10152
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TRPC1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
48 HOMER2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 HNRNPLL 92906
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 EAF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
51 PEX14 5195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 Snw1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
53 PPP1R18 170954
Two-hybrid Homo sapiens
54 PSMC5 5705
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
55 PKN1 5585
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 PML 5371
Affinity Capture-MS Homo sapiens
57 Homer1  
Affinity Capture-MS Mus musculus
58 WASF2 10163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 ABI1 10006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SNRPF 6636
Two-hybrid Homo sapiens
61 FBXO28  
Affinity Capture-MS Homo sapiens
62 IKBKB 3551
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
63 POM121  
Two-hybrid Homo sapiens
64 Pax6  
Two-hybrid Mus musculus
65 IFIT2 3433
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 EIF3D 8664
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here