Experiment description of studies that identified PAFAH1B3 in exosomes

1 Experiment ID 224 ISEV standards ✔ EM|AFM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81 EV Membrane markers ✔ GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 191 ISEV standards ✘ EV Biophysical techniques ✔ Alix EV Cytosolic markers ✔ CD81|CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20124223     Organism Homo sapiens Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. Journal name Mol Cell Proteomics Publication year 2010 Sample Squamous carcinoma cells Sample name Squamous carcinoma cell (A431) Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 217 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ CD81|CD9|CD63 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for PAFAH1B3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PAFAH1B1 5048 Two-hybrid Homo sapiens Invitro Homo sapiens 2 BRD7   Two-hybrid Homo sapiens 3 ECH1 1891 Two-hybrid Homo sapiens 4 MGC15730   Two-hybrid Homo sapiens 5 DDX24 57062 Two-hybrid Homo sapiens 6 TP53   Two-hybrid Homo sapiens 7 TUBB2A 7280 Two-hybrid Homo sapiens 8 TLE1   Two-hybrid Homo sapiens 9 EEF1A1 1915 Two-hybrid Homo sapiens 10 PAFAH1B3 5050 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 11 C14orf1 11161 Two-hybrid Homo sapiens 12 ZHX1   Two-hybrid Homo sapiens 13 RIF1   Two-hybrid Homo sapiens 14 DKFZP564O0523   Two-hybrid Homo sapiens 15 PAFAH1B2 5049 Two-hybrid Homo sapiens 16 SDCBP2 27111 Two-hybrid Homo sapiens 17 GDF9 2661 Two-hybrid Homo sapiens 18 UNC119   Two-hybrid Homo sapiens 19 ZBTB16   Two-hybrid Homo sapiens 20 GBP2   Two-hybrid Homo sapiens 21 SETDB1   Two-hybrid Homo sapiens 22 MDS025   Two-hybrid Homo sapiens 23 GAPDH 2597 Two-hybrid Homo sapiens 24 XRCC6 2547 Two-hybrid Homo sapiens 25 LNX   Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 26 COPS6 10980 Two-hybrid Homo sapiens 27 PLEKHM1   Two-hybrid Homo sapiens 28 HSPH1 10808 Reconstituted Complex Homo sapiens 29 CRMP1 1400 Two-hybrid Homo sapiens 30 MED31   Two-hybrid Homo sapiens 31 CHMP7   Two-hybrid Homo sapiens View the network

Pathways in which PAFAH1B3 is involved

No pathways found