Gene description for NCKAP1L
Gene name NCK-associated protein 1-like
Gene symbol NCKAP1L
Other names/aliases HEM1
Species Homo sapiens
 Database cross references - NCKAP1L
ExoCarta ExoCarta_3071
Vesiclepedia VP_3071
Entrez Gene 3071
HGNC 4862
MIM 141180
UniProt P55160  
 NCKAP1L identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for NCKAP1L
Molecular Function
    GTPase activator activity GO:0005096 IMP
    protein binding GO:0005515 IPI
    protein kinase activator activity GO:0030295 IMP
    protein-containing complex binding GO:0044877 IDA
    TORC2 complex binding GO:1904841 IDA
Biological Process
    cell morphogenesis GO:0000902 IBA
    B cell homeostasis GO:0001782 ISS
    myeloid cell homeostasis GO:0002262 ISS
    positive regulation of leukocyte migration GO:0002687 IMP
    chemotaxis GO:0006935 IDA
    response to xenobiotic stimulus GO:0009410 IMP
    cell migration GO:0016477 IBA
    maintenance of cell polarity GO:0030011 IMP
    cell projection assembly GO:0030031 IBA
    positive regulation of actin filament polymerization GO:0030838 IMP
    cortical actin cytoskeleton organization GO:0030866 IBA
    cortical actin cytoskeleton organization GO:0030866 IMP
    cortical actin cytoskeleton organization GO:0030866 ISS
    positive regulation of B cell proliferation GO:0030890 IMP
    negative regulation of interleukin-17 production GO:0032700 ISS
    negative regulation of interleukin-6 production GO:0032715 ISS
    positive regulation of cell adhesion mediated by integrin GO:0033630 ISS
    erythrocyte homeostasis GO:0034101 ISS
    intracellular signal transduction GO:0035556 IMP
    positive regulation of T cell proliferation GO:0042102 IMP
    positive regulation of T cell proliferation GO:0042102 ISS
    T cell homeostasis GO:0043029 ISS
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of cytotoxic T cell degranulation GO:0043318 IMP
    positive regulation of CD4-positive, alpha-beta T cell differentiation GO:0043372 ISS
    positive regulation of CD8-positive, alpha-beta T cell differentiation GO:0043378 ISS
    positive regulation of B cell differentiation GO:0045579 ISS
    positive regulation of gamma-delta T cell differentiation GO:0045588 ISS
    positive regulation of lymphocyte differentiation GO:0045621 ISS
    positive regulation of erythrocyte differentiation GO:0045648 ISS
    neuron projection morphogenesis GO:0048812 IBA
    erythrocyte development GO:0048821 IEP
    B cell receptor signaling pathway GO:0050853 IMP
    positive regulation of phagocytosis, engulfment GO:0060100 ISS
    protein-containing complex assembly GO:0065003 ISS
    actin polymerization-dependent cell motility GO:0070358 IMP
    positive regulation of neutrophil chemotaxis GO:0090023 ISS
    positive regulation of neutrophil migration GO:1902624 IMP
    positive regulation of TORC2 signaling GO:1904515 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    secretory granule membrane GO:0030667 TAS
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 IDA
    extracellular exosome GO:0070062 HDA
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified NCKAP1L in exosomes
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCKAP1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRK1 55845
Affinity Capture-MS Homo sapiens
2 CYFIP1 23191
Co-fractionation Homo sapiens
3 ABI2 10152
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ABI1 10006
Co-fractionation Homo sapiens
5 ANXA2 302
Co-fractionation Homo sapiens
6 GAS7  
Reconstituted Complex Homo sapiens
7 CYFIP2 26999
Co-fractionation Homo sapiens
8 NOTCH1 4851
Affinity Capture-MS Homo sapiens
9 PRPF40A 55660
Reconstituted Complex Homo sapiens
10 DDX58 23586
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which NCKAP1L is involved
PathwayEvidenceSource
Disease TAS Reactome
Disease IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
FCGR3A-mediated phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis IEA Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Leishmania infection TAS Reactome
Leishmania infection IEA Reactome
Leishmania phagocytosis TAS Reactome
Leishmania phagocytosis IEA Reactome
Neutrophil degranulation TAS Reactome
Parasite infection TAS Reactome
Parasite infection IEA Reactome
Parasitic Infection Pathways TAS Reactome
Parasitic Infection Pathways IEA Reactome
RAC1 GTPase cycle TAS Reactome
RAC2 GTPase cycle TAS Reactome
RAC3 GTPase cycle TAS Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate WASPs and WAVEs TAS Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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