Gene description for CFB
Gene name complement factor B
Gene symbol CFB
Other names/aliases AHUS4
ARMD14
BF
BFD
CFAB
CFBD
FB
FBI12
GBG
H2-Bf
PBF2
Species Homo sapiens
 Database cross references - CFB
ExoCarta ExoCarta_629
Entrez Gene 629
HGNC 1037
MIM 138470
UniProt P00751  
 CFB identified in exosomes derived from the following tissue/cell type
Keratinocytes 19530224    
Keratinocytes 19530224    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CFB
Molecular Function
    serine-type endopeptidase activity GO:0004252 TAS
    complement binding GO:0001848 TAS
Biological Process
    regulation of complement activation GO:0030449 TAS
    innate immune response GO:0045087 TAS
    proteolysis GO:0006508 IEA
    complement activation, alternative pathway GO:0006957 TAS
    complement activation GO:0006956 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    extracellular space GO:0005615 IDA
    blood microparticle GO:0072562 IDA
    extracellular region GO:0005576 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CFB in exosomes
1
Experiment ID 189
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 190
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CFB
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CFB is involved
PathwayEvidenceSource
Activation of C3 and C5 TAS Reactome
Alternative complement activation TAS Reactome
Regulation of Complement cascade TAS Reactome





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