Gene description for TRIM69
Gene name tripartite motif containing 69
Gene symbol TRIM69
Other names/aliases HSD-34
HSD34
RNF36
Trif
Species Homo sapiens
 Database cross references - TRIM69
ExoCarta ExoCarta_140691
Vesiclepedia VP_140691
Entrez Gene 140691
HGNC 17857
MIM 616017
UniProt Q86WT6  
 TRIM69 identified in exosomes derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for TRIM69
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    apoptotic process GO:0006915 IEA
    protein ubiquitination GO:0016567 IEA
    innate immune response GO:0045087 IBA
    protein localization to centrosome GO:0071539 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified TRIM69 in exosomes
1
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRIM69
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RRM1 6240
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Reconstituted Complex Homo sapiens
3 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
4 TRIM63  
Two-hybrid Homo sapiens
5 DVL1 1855
Two-hybrid Homo sapiens
6 ZNF483  
Two-hybrid Homo sapiens
7 RINT1 60561
Two-hybrid Homo sapiens
8 RBFA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TRIM4 89122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MED20 9477
Two-hybrid Homo sapiens
11 HAUS1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 GJA1 2697
Affinity Capture-MS Homo sapiens
14 ASNS 440
Two-hybrid Homo sapiens
15 C11orf85  
Two-hybrid Homo sapiens
16 KRT17 3872
Two-hybrid Homo sapiens
17 RSPH14  
Two-hybrid Homo sapiens
18 UBE2N 7334
Reconstituted Complex Homo sapiens
19 TNNT2  
Two-hybrid Homo sapiens
20 KLHL38  
Two-hybrid Homo sapiens
21 LARP1B 55132
Two-hybrid Homo sapiens
22 CDSN 1041
Two-hybrid Homo sapiens
23 BEX2  
Two-hybrid Homo sapiens
24 SF1 7536
Two-hybrid Homo sapiens
25 UBE2E1  
Reconstituted Complex Homo sapiens
26 SYCE2  
Two-hybrid Homo sapiens
27 WWOX 51741
Two-hybrid Homo sapiens
28 POLR3K  
Two-hybrid Homo sapiens
29 FAM90A1  
Two-hybrid Homo sapiens
30 FAM110A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 DDX58 23586
Affinity Capture-RNA Homo sapiens
32 BLOC1S5 63915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 APP 351
Reconstituted Complex Homo sapiens
34 STMN3  
Two-hybrid Homo sapiens
35 TRIM54  
Two-hybrid Homo sapiens
36 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 POTEF 728378
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TLR9  
Affinity Capture-Western Homo sapiens
39 ATP5G1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 TRIM55  
Two-hybrid Homo sapiens
41 SYNE4  
Two-hybrid Homo sapiens
42 GEM  
Two-hybrid Homo sapiens
43 TRIM44  
Two-hybrid Homo sapiens
44 SNAPIN 23557
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 LYSMD1  
Two-hybrid Homo sapiens
46 Trim69  
Affinity Capture-MS Mus musculus
47 BLOC1S2 282991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FAM161A  
Two-hybrid Homo sapiens
49 TLR3  
Affinity Capture-Western Homo sapiens
50 MAGEA4 4103
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
51 NEK8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
52 BIN3 55909
Two-hybrid Homo sapiens
53 SNX31  
Affinity Capture-MS Homo sapiens
54 HGS 9146
Two-hybrid Homo sapiens
55 TRIM69 140691
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 PPP1R18 170954
Two-hybrid Homo sapiens
57 ANXA9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RECK 8434
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 C4orf45  
Two-hybrid Homo sapiens
60 ETFDH 2110
Two-hybrid Homo sapiens
61 PML 5371
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 UBE2D3 7323
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
63 EYA4 2070
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
64 FKBP1B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
65 WASH1 100287171
Two-hybrid Homo sapiens
66 TLR4  
Affinity Capture-Western Homo sapiens
67 SF3A3 10946
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
68 IYD  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
69 UBE2D1 7321
Reconstituted Complex Homo sapiens
70 WDYHV1  
Two-hybrid Homo sapiens
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