Gene description for BCAT1
Gene name branched chain amino-acid transaminase 1, cytosolic
Gene symbol BCAT1
Other names/aliases BCATC
BCT1
ECA39
MECA39
PNAS121
PP18
Species Homo sapiens
 Database cross references - BCAT1
ExoCarta ExoCarta_586
Vesiclepedia VP_586
Entrez Gene 586
HGNC 976
MIM 113520
UniProt P54687  
 BCAT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for BCAT1
Molecular Function
    branched-chain-amino-acid transaminase activity GO:0004084 IBA
    protein binding GO:0005515 IPI
    L-leucine-2-oxoglutarate transaminase activity GO:0052654 ISS
    L-valine-2-oxoglutarate transaminase activity GO:0052655 ISS
    L-isoleucine-2-oxoglutarate transaminase activity GO:0052656 ISS
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    lipid metabolic process GO:0006629 IEA
    branched-chain amino acid biosynthetic process GO:0009082 TAS
    L-leucine biosynthetic process GO:0009098 IBA
    L-valine biosynthetic process GO:0009099 IBA
Subcellular Localization
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified BCAT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for BCAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YEATS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GTF2A1 2957
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 LDHA 3939
Co-fractionation Homo sapiens
4 GTF2F1 2962
Co-fractionation Homo sapiens
5 SMAD5 4090
Two-hybrid Homo sapiens
6 AGL 178
Co-fractionation Homo sapiens
7 ADPRH 141
Affinity Capture-MS Homo sapiens
8 PER1  
Affinity Capture-MS Homo sapiens
9 PRMT6  
Two-hybrid Homo sapiens
10 FBXO7 25793
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LRIF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PRNP 5621
Affinity Capture-MS Homo sapiens
13 TCF7L2  
Affinity Capture-MS Homo sapiens
14 EPHX2 2053
Co-fractionation Homo sapiens
15 ATXN2 6311
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PRR14L 253143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GPX1 2876
Co-fractionation Homo sapiens
18 CCDC120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ZNHIT2 741
Affinity Capture-MS Homo sapiens
20 FLYWCH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PRKAR1B  
Affinity Capture-MS Homo sapiens
22 USP15 9958
Affinity Capture-MS Homo sapiens
23 GFOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CUL3 8452
Affinity Capture-MS Homo sapiens
25 ARNT 405
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ZZZ3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 DMWD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 EIF4G3 8672
Affinity Capture-MS Homo sapiens
29 TRIM55  
Two-hybrid Homo sapiens
30 TRIP12 9320
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ARID3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SNF8 11267
Co-fractionation Homo sapiens
33 SPARC 6678
Co-fractionation Homo sapiens
34 RNF126  
Affinity Capture-MS Homo sapiens
35 FAM213B 127281
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DUS3L 56931
Co-fractionation Homo sapiens
37 MIF 4282
Affinity Capture-MS Homo sapiens
38 KDM1A 23028
Two-hybrid Homo sapiens
39 SERPINE2 5270
Co-fractionation Homo sapiens
40 PJA1  
Affinity Capture-MS Homo sapiens
41 HIBADH 11112
Co-fractionation Homo sapiens
42 PPP2R5D 5528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 HELZ 9931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PSAP 5660
Co-fractionation Homo sapiens
45 ACADM 34
Co-fractionation Homo sapiens
46 MORN5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TRMT44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RAB3GAP1 22930
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 INPP5B 3633
Co-fractionation Homo sapiens
50 PUS1 80324
Co-fractionation Homo sapiens
51 CEBPA  
Protein-peptide Homo sapiens
52 TRIM63  
Two-hybrid Homo sapiens
53 ATIC 471
Co-fractionation Homo sapiens
54 ACAA2 10449
Co-fractionation Homo sapiens
55 PROSER2 254427
Affinity Capture-MS Homo sapiens
56 PAPLN  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which BCAT1 is involved
PathwayEvidenceSource
Branched-chain amino acid catabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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