Gene description for LCN2
Gene name lipocalin 2
Gene symbol LCN2
Other names/aliases 24p3
MSFI
NGAL
Species Homo sapiens
 Database cross references - LCN2
ExoCarta ExoCarta_3934
Entrez Gene 3934
HGNC 6526
MIM 600181
UniProt P80188  
 LCN2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Urine 19056867    
 Gene ontology annotations for LCN2
Molecular Function
    iron ion binding GO:0005506 ISS
    small molecule binding GO:0036094 IEA
    transporter activity GO:0005215 IEA
Biological Process
    cellular iron ion homeostasis GO:0006879 TAS
    innate immune response GO:0045087 ISS
    siderophore transport GO:0015891 ISS
    apoptotic process GO:0006915 IEA
    transmembrane transport GO:0055085 TAS
    ion transport GO:0006811 IEA
Subcellular Localization
    extracellular space GO:0005615 IDA
    cell GO:0005623 TAS
    extracellular exosome GO:0070062 IDA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified LCN2 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
3
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for LCN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LCN2 3934
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 MMP2 4313
Affinity Capture-Western Homo sapiens
3 MMP9 4318
Invitro Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which LCN2 is involved
PathwayEvidenceSource
Iron uptake and transport TAS Reactome





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