ExoCarta: Gene summary

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Gene description for SEC61G
Gene name	Sec61 gamma subunit
Gene symbol	SEC61G
Other names/aliases	SSS1
Species	Homo sapiens
Database cross references - SEC61G
ExoCarta	ExoCarta_23480
Vesiclepedia	VP_23480
Entrez Gene	23480
HGNC	18277
MIM	609215
UniProt	P60059
SEC61G identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for SEC61G

Molecular Function
	protein binding	GO:0005515	IPI
	protein transmembrane transporter activity	GO:0008320	IBA
	protein transmembrane transporter activity	GO:0008320	ISS
	ribosome binding	GO:0043022	IDA
Biological Process
	post-translational protein targeting to membrane, translocation	GO:0031204	IBA
	protein targeting to ER	GO:0045047	ISS
Subcellular Localization
	cytosol	GO:0005829	TAS
	membrane	GO:0016020	IDA
	Ssh1 translocon complex	GO:0071261	IBA

Experiment description of studies that identified SEC61G in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for SEC61G

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

SRP19

6728

Affinity Capture-MS

Homo sapiens

TMEM51

55092

Two-hybrid

Homo sapiens

RBM42

Affinity Capture-MS

Homo sapiens

RBM39

9584

Affinity Capture-MS

Homo sapiens

SERBP1

26135

Affinity Capture-MS

Homo sapiens

CSNK2B

1460

Affinity Capture-MS

Homo sapiens

ILF3

3609

Affinity Capture-MS

Homo sapiens

CANX

821

Affinity Capture-MS

Homo sapiens

RBM8A

9939

Affinity Capture-MS

Homo sapiens

LRRC25

Two-hybrid

Homo sapiens

AURKA

6790

Affinity Capture-MS

Homo sapiens

SRP9

6726

Affinity Capture-MS

Homo sapiens

TUBGCP3

10426

Affinity Capture-MS

Homo sapiens

PEX12

Two-hybrid

Homo sapiens

EGFR

1956

Negative Genetic

Homo sapiens

DRG1

4733

Affinity Capture-MS

Homo sapiens

DDX58

23586

Affinity Capture-RNA

Homo sapiens

CREB3L1

Two-hybrid

Homo sapiens

MAPRE1

22919

Affinity Capture-MS

Homo sapiens

TOP2A

7153

Affinity Capture-MS

Homo sapiens

SFTPC

Two-hybrid

Homo sapiens

FAM209A

200232

Two-hybrid

Homo sapiens

FKBP5

2289

Cross-Linking-MS (XL-MS)

Homo sapiens

RPL5

6125

Affinity Capture-MS

Homo sapiens

PSPC1

55269

Affinity Capture-MS

Homo sapiens

RPA2

6118

Proximity Label-MS

Homo sapiens

ANK2

287

Cross-Linking-MS (XL-MS)

Homo sapiens

CREB3

Two-hybrid

Homo sapiens

KRAS

3845

Negative Genetic

Homo sapiens

GNB2L1

10399

Affinity Capture-MS

Homo sapiens

LCN2

3934

Two-hybrid

Homo sapiens

SEC61A1

29927

Reconstituted Complex

Homo sapiens

RPA3

6119

Proximity Label-MS

Homo sapiens

CCDC47

57003

Affinity Capture-MS

Homo sapiens

SEC61B

10952

Affinity Capture-MS

Homo sapiens

RPS16

6217

Affinity Capture-MS

Homo sapiens

SRP68

6730

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which SEC61G is involved

Pathway	Evidence	Source
Adaptive Immune System	IEA	Reactome
Antigen processing-Cross presentation	IEA	Reactome
Class I MHC mediated antigen processing & presentation	IEA	Reactome
ER-Phagosome pathway	IEA	Reactome
Immune System	IEA	Reactome
Insertion of tail-anchored proteins into the endoplasmic reticulum membrane	TAS	Reactome
Insertion of tail-anchored proteins into the endoplasmic reticulum membrane	IEA	Reactome
Metabolism of proteins	IEA	Reactome
Protein localization	TAS	Reactome
Protein localization	IEA	Reactome
SRP-dependent cotranslational protein targeting to membrane	IEA	Reactome
Translation	IEA	Reactome

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