Gene description for IKBKE
Gene name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
Gene symbol IKBKE
Other names/aliases IKK-E
IKK-i
IKKE
IKKI
Species Homo sapiens
 Database cross references - IKBKE
ExoCarta ExoCarta_9641
Vesiclepedia VP_9641
Entrez Gene 9641
HGNC 14552
MIM 605048
UniProt Q14164  
 IKBKE identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for IKBKE
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    IkappaB kinase activity GO:0008384 IEA
    protein phosphatase binding GO:0019903 ISS
    ubiquitin protein ligase binding GO:0031625 IPI
    K48-linked polyubiquitin modification-dependent protein binding GO:0036435 IMP
    identical protein binding GO:0042802 IPI
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IMP
Biological Process
    activation of innate immune response GO:0002218 IBA
    immune response GO:0006955 NAS
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IMP
    gene expression GO:0010467 IEA
    positive regulation of lipid storage GO:0010884 ISS
    positive regulation of type I interferon production GO:0032481 IBA
    positive regulation of type I interferon production GO:0032481 IDA
    response to interferon-beta GO:0035456 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    regulation of protein-containing complex assembly GO:0043254 IEA
    mRNA stabilization GO:0048255 IEA
    defense response to virus GO:0051607 NAS
    type I interferon-mediated signaling pathway GO:0060337 NAS
    positive regulation of type I interferon-mediated signaling pathway GO:0060340 IDA
    interleukin-17-mediated signaling pathway GO:0097400 IEA
    cellular response to virus GO:0098586 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    PML body GO:0016605 IDA
    mitochondrial membrane GO:0031966 IMP
    serine/threonine protein kinase complex GO:1902554 NAS
 Experiment description of studies that identified IKBKE in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IKBKE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 RELA 5970
Biochemical Activity Homo sapiens
3 BIRC2  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
4 MAVS 57506
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 EIF4B 1975
Affinity Capture-MS Homo sapiens
6 TNIP1 10318
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 TRAF3IP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
8 XIAP  
Biochemical Activity Homo sapiens
9 TRAF2 7186
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 PLS3 5358
Affinity Capture-MS Homo sapiens
11 MBP 4155
Biochemical Activity Homo sapiens
12 CCT5 22948
Affinity Capture-MS Homo sapiens
13 SNAI1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 RNF11 26994
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 TAX1BP1 8887
Affinity Capture-Western Homo sapiens
16 HSPA8 3312
Affinity Capture-MS Homo sapiens
17 TBKBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
18 RUVBL2 10856
Affinity Capture-MS Homo sapiens
19 DDX3X 1654
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 CD209 30835
Affinity Capture-Western Homo sapiens
21 NFKBIA  
Biochemical Activity Homo sapiens
22 CCT6A 908
Affinity Capture-MS Homo sapiens
23 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 IKBKG 8517
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
25 FKBP5 2289
Affinity Capture-MS Homo sapiens
26 NMRAL1 57407
Affinity Capture-Western Homo sapiens
27 UBC 7316
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 ROCK2 9475
Affinity Capture-MS Homo sapiens
29 CCT8 10694
Affinity Capture-MS Homo sapiens
30 CCT2 10576
Affinity Capture-MS Homo sapiens
31 PSMD14 10213
Affinity Capture-Western Homo sapiens
32 MAP4K1  
Affinity Capture-Western Homo sapiens
33 TRIM6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
35 EPRS 2058
Affinity Capture-MS Homo sapiens
36 DDX58 23586
Affinity Capture-Western Homo sapiens
37 TRAF3 7187
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 LARS 51520
Affinity Capture-MS Homo sapiens
39 TANK 10010
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
40 ESR1  
Affinity Capture-Western Homo sapiens
41 APPBP2  
Affinity Capture-MS Homo sapiens
42 LRPPRC 10128
Affinity Capture-MS Homo sapiens
43 IRF7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 TBK1 29110
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
45 AZI2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
46 DSTYK 25778
Affinity Capture-MS Homo sapiens
47 HSP90AA2P 3324
Affinity Capture-MS Homo sapiens
48 PABPC1 26986
Affinity Capture-MS Homo sapiens
49 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
50 IMPDH2 3615
Affinity Capture-MS Homo sapiens
51 CCT4 10575
Affinity Capture-MS Homo sapiens
52 VCP 7415
Affinity Capture-MS Homo sapiens
53 PSMA7 5688
Affinity Capture-MS Homo sapiens
54 BCL10  
Affinity Capture-MS Homo sapiens
55 MYD88 4615
Affinity Capture-Western Homo sapiens
56 TRIM27  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
57 HYOU1 10525
Affinity Capture-MS Homo sapiens
58 LCP1 3936
Affinity Capture-MS Homo sapiens
59 TOPORS  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
60 SND1 27044
Affinity Capture-MS Homo sapiens
61 CCT3 7203
Affinity Capture-MS Homo sapiens
62 PML 5371
Co-localization Homo sapiens
63 KTN1 3895
Affinity Capture-MS Homo sapiens
64 CLTC 1213
Affinity Capture-MS Homo sapiens
65 CALCOCO2  
Affinity Capture-Western Homo sapiens
66 IFIT3 3437
Affinity Capture-Western Homo sapiens
67 MYH10 4628
Affinity Capture-MS Homo sapiens
68 IKBKB 3551
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
69 CHUK 1147
Biochemical Activity Homo sapiens
70 ACLY 47
Affinity Capture-MS Homo sapiens
71 IKBKE 9641
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
72 IRF3 3661
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
73 CYLD  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
74 TCP1 6950
Affinity Capture-MS Homo sapiens
75 LRRC10B  
Affinity Capture-MS Homo sapiens
76 ATIC 471
Affinity Capture-MS Homo sapiens
77 PDIA6 10130
Affinity Capture-MS Homo sapiens
78 BIRC3 330
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which IKBKE is involved
PathwayEvidenceSource
Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) TAS Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
DDX58/IFIH1-mediated induction of interferon-alpha/beta TAS Reactome
Death Receptor Signaling TAS Reactome
Disease TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Metabolism of proteins TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
Negative regulators of DDX58/IFIH1 signaling TAS Reactome
Post-translational protein modification TAS Reactome
Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 TAS Reactome
Regulation of TBK1, IKKε-mediated activation of IRF3, IRF7 upon TLR3 ligation TAS Reactome
Regulation of TNFR1 signaling TAS Reactome
Response of endothelial cells to shear stress TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 activates/modulates innate immune responses TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-1-host interactions TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Signal Transduction TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMOylation TAS Reactome
SUMOylation of immune response proteins TAS Reactome
TICAM1-dependent activation of IRF3/IRF7 TAS Reactome
TNF signaling TAS Reactome
TNFR1-induced proapoptotic signaling TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF3-dependent IRF activation pathway TAS Reactome
TRAF6 mediated IRF7 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome
Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells TAS Reactome
Viral Infection Pathways TAS Reactome





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