Gene description for CMAS
Gene name cytidine monophosphate N-acetylneuraminic acid synthetase
Gene symbol CMAS
Other names/aliases CSS
Species Homo sapiens
 Database cross references - CMAS
ExoCarta ExoCarta_55907
Vesiclepedia VP_55907
Entrez Gene 55907
HGNC 18290
MIM 603316
UniProt Q8NFW8  
 CMAS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CMAS
Molecular Function
    N-acylneuraminate cytidylyltransferase activity GO:0008781 IBA
Biological Process
    N-acetylneuraminate metabolic process GO:0006054 IEA
    CMP-N-acetylneuraminate biosynthetic process GO:0006055 IMP
    glycosylation GO:0070085 IMP
Subcellular Localization
    nucleus GO:0005634 HDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified CMAS in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CMAS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FIG4 9896
Affinity Capture-MS Homo sapiens
2 ANKRD49  
Affinity Capture-MS Homo sapiens
3 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HECTD1 25831
Affinity Capture-MS Homo sapiens
5 SOST  
Affinity Capture-MS Homo sapiens
6 MECP2 4204
Affinity Capture-MS Homo sapiens
7 MRPL12 6182
Affinity Capture-MS Homo sapiens
8 ANAPC16  
Affinity Capture-MS Homo sapiens
9 RNASEH1 246243
Affinity Capture-MS Homo sapiens
10 FAM98A 25940
Affinity Capture-MS Homo sapiens
11 RDX 5962
Co-fractionation Homo sapiens
12 MED17  
Affinity Capture-MS Homo sapiens
13 UBE2H 7328
Affinity Capture-MS Homo sapiens
14 RPL19 6143
Affinity Capture-MS Homo sapiens
15 DNAJC19 131118
Affinity Capture-MS Homo sapiens
16 FBXL6  
Affinity Capture-MS Homo sapiens
17 MTPN 136319
Affinity Capture-MS Homo sapiens
18 ATP5F1 515
Affinity Capture-MS Homo sapiens
19 YTHDF1 54915
Affinity Capture-MS Homo sapiens
20 INSIG2  
Affinity Capture-MS Homo sapiens
21 RPA2 6118
Proximity Label-MS Homo sapiens
22 EXTL3 2137
Affinity Capture-MS Homo sapiens
23 EIF2B2 8892
Affinity Capture-MS Homo sapiens
24 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
25 MATN2 4147
Affinity Capture-MS Homo sapiens
26 NAA15 80155
Co-fractionation Homo sapiens
27 BRCA1 672
Affinity Capture-MS Homo sapiens
28 SRP19 6728
Affinity Capture-MS Homo sapiens
29 NGEF  
Affinity Capture-MS Homo sapiens
30 DBNL 28988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
32 NPM1 4869
Affinity Capture-MS Homo sapiens
33 RPL4 6124
Affinity Capture-MS Homo sapiens
34 FBXO6 26270
Affinity Capture-MS Homo sapiens
35 AGPAT3 56894
Affinity Capture-MS Homo sapiens
36 MILR1  
Affinity Capture-MS Homo sapiens
37 DNAJC15  
Affinity Capture-MS Homo sapiens
38 RAD1  
Affinity Capture-MS Homo sapiens
39 APP 351
Reconstituted Complex Homo sapiens
40 DNAJB7  
Affinity Capture-MS Homo sapiens
41 PRDM5  
Affinity Capture-MS Homo sapiens
42 UFL1 23376
Affinity Capture-MS Homo sapiens
43 RPL28 6158
Affinity Capture-MS Homo sapiens
44 PRKCSH 5589
Affinity Capture-MS Homo sapiens
45 PARK2  
Affinity Capture-MS Homo sapiens
46 SNX33 257364
Affinity Capture-MS Homo sapiens
47 REG3A  
Affinity Capture-MS Homo sapiens
48 USP36  
Affinity Capture-MS Homo sapiens
49 TRIM28 10155
Affinity Capture-MS Homo sapiens
50 Srp72  
Affinity Capture-MS Mus musculus
51 RPA3 6119
Proximity Label-MS Homo sapiens
52 ZMYND8 23613
Affinity Capture-MS Homo sapiens
53 CLOCK  
Affinity Capture-MS Homo sapiens
54 ZCCHC10  
Affinity Capture-MS Homo sapiens
55 LINGO2  
Affinity Capture-MS Homo sapiens
56 SSSCA1 10534
Affinity Capture-MS Homo sapiens
57 CHCHD2  
Affinity Capture-MS Homo sapiens
58 Cep170  
Affinity Capture-MS Mus musculus
59 GRSF1 2926
Proximity Label-MS Homo sapiens
60 C1QBP 708
Proximity Label-MS Homo sapiens
61 CTNNA3  
Affinity Capture-MS Homo sapiens
62 DDB1 1642
Co-fractionation Homo sapiens
63 COG3 83548
Affinity Capture-MS Homo sapiens
64 DNAJC1 64215
Affinity Capture-MS Homo sapiens
65 CHCHD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 GNB2L1 10399
Affinity Capture-MS Homo sapiens
67 DDRGK1 65992
Affinity Capture-MS Homo sapiens
68 RC3H1 149041
Affinity Capture-MS Homo sapiens
69 MRPL11 65003
Proximity Label-MS Homo sapiens
70 C1qbp 12261
Affinity Capture-MS Mus musculus
71 MRPL38  
Affinity Capture-MS Homo sapiens
72 PTCH1  
Affinity Capture-MS Homo sapiens
73 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 NAA10 8260
Co-fractionation Homo sapiens
75 LIMS1 3987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 DNAJC22  
Affinity Capture-MS Homo sapiens
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