Gene description for RCN3
Gene name reticulocalbin 3, EF-hand calcium binding domain
Gene symbol RCN3
Other names/aliases RLP49
Species Homo sapiens
 Database cross references - RCN3
ExoCarta ExoCarta_57333
Vesiclepedia VP_57333
Entrez Gene 57333
HGNC 21145
UniProt Q96D15  
 RCN3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RCN3
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
Biological Process
    protein secretion GO:0009306 IMP
    positive regulation of peptidase activity GO:0010952 IMP
    protein transport GO:0015031 ISS
    collagen biosynthetic process GO:0032964 IMP
    ERAD pathway GO:0036503 ISS
    surfactant homeostasis GO:0043129 ISS
    regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051896 IMP
    phospholipid homeostasis GO:0055091 ISS
    lung epithelium development GO:0060428 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified RCN3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RCN3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 E2F4  
Affinity Capture-MS Homo sapiens
2 MYO15B 80022
Two-hybrid Homo sapiens
3 AURKB 9212
Affinity Capture-MS Homo sapiens
4 SUFU 51684
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
5 PRUNE2  
Two-hybrid Homo sapiens
6 XRCC3  
Affinity Capture-MS Homo sapiens
7 MGARP  
Co-fractionation Homo sapiens
8 MBD3 53615
Two-hybrid Homo sapiens
9 STAT6 6778
Two-hybrid Homo sapiens
10 PDE4B  
Protein-RNA Homo sapiens
11 CCDC103  
Two-hybrid Homo sapiens
12 ZNF417  
Two-hybrid Homo sapiens
13 KLHL42  
Two-hybrid Homo sapiens
14 NAA10 8260
Two-hybrid Homo sapiens
15 HID1 283987
Two-hybrid Homo sapiens
16 MKNK1  
Two-hybrid Homo sapiens
17 PACRGL  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RCN3 is involved
No pathways found





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