Experiment description of studies that identified KCTD12 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for KCTD12

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ENDOG   Affinity Capture-MS Homo sapiens 2 RIPK2   Affinity Capture-MS Homo sapiens 3 GABBR2   Reconstituted Complex Homo sapiens 4 GTF2F1 2962 Affinity Capture-MS Homo sapiens 5 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 6 CHTF8   Affinity Capture-MS Homo sapiens 7 LATS1   Affinity Capture-MS Homo sapiens 8 FBXL6   Affinity Capture-MS Homo sapiens 9 KPNA1 3836 Co-fractionation Homo sapiens 10 RPS4X 6191 Affinity Capture-MS Homo sapiens 11 YTHDF1 54915 Affinity Capture-MS Homo sapiens 12 TPD52L1 7164 Affinity Capture-MS Homo sapiens 13 RPA2 6118 Proximity Label-MS Homo sapiens 14 KCTD12 115207 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 15 DLD 1738 Affinity Capture-MS Homo sapiens 16 ZIC2   Affinity Capture-MS Homo sapiens 17 CD274 29126 Affinity Capture-MS Homo sapiens 18 CUL3 8452 Affinity Capture-MS Homo sapiens 19 ARHGEF39 84904 Affinity Capture-MS Homo sapiens 20 KCTD16   Affinity Capture-MS Homo sapiens 21 OTUB1 55611 Affinity Capture-MS Homo sapiens 22 PRR5   Affinity Capture-MS Homo sapiens 23 GNE 10020 Co-fractionation Homo sapiens 24 ZNF792 126375 Two-hybrid Homo sapiens 25 Abr 109934 Affinity Capture-MS Mus musculus 26 KIAA1598 57698 Co-fractionation Homo sapiens 27 CLTCL1 8218 Affinity Capture-MS Homo sapiens 28 Ksr1   Affinity Capture-MS Mus musculus 29 GNG2 54331 Affinity Capture-MS Homo sapiens 30 RCCD1   Affinity Capture-MS Homo sapiens 31 RPA3 6119 Proximity Label-MS Homo sapiens 32 TSR3   Affinity Capture-MS Homo sapiens 33 RPL23 9349 Affinity Capture-MS Homo sapiens 34 NUDT16 131870 Affinity Capture-MS Homo sapiens 35 DNPEP 23549 Co-fractionation Homo sapiens 36 OSBP 5007 Co-fractionation Homo sapiens 37 NCL 4691 Co-fractionation Homo sapiens 38 PYCRL 65263 Affinity Capture-MS Homo sapiens 39 RPL24 6152 Affinity Capture-MS Homo sapiens 40 DUSP11   Affinity Capture-MS Homo sapiens 41 DDRGK1 65992 Affinity Capture-MS Homo sapiens 42 PUSL1   Affinity Capture-MS Homo sapiens 43 PIF1   Affinity Capture-MS Homo sapiens 44 SMURF2 64750 Affinity Capture-MS Homo sapiens 45 GABPA 2551 Co-fractionation Homo sapiens 46 LMNA 4000 Co-fractionation Homo sapiens 47 RANBP6 26953 Co-fractionation Homo sapiens 48 DNM1L 10059 Affinity Capture-MS Homo sapiens 49 RASSF5 83593 Affinity Capture-MS Homo sapiens 50 ARAP1 116985 Two-hybrid Homo sapiens 51 DNAH14   Affinity Capture-MS Homo sapiens View the network

Pathways in which KCTD12 is involved

No pathways found