Gene description for ATP6V0A4
Gene name ATPase, H+ transporting, lysosomal V0 subunit a4
Gene symbol ATP6V0A4
Other names/aliases A4
ATP6N1B
ATP6N2
RDRTA2
RTA1C
RTADR
STV1
VPH1
VPP2
Species Homo sapiens
 Database cross references - ATP6V0A4
ExoCarta ExoCarta_50617
Vesiclepedia VP_50617
Entrez Gene 50617
HGNC 866
MIM 605239
UniProt Q9HBG4  
 ATP6V0A4 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ATP6V0A4
Molecular Function
    protein binding GO:0005515 IPI
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    ATPase binding GO:0051117 IBA
    ATPase binding GO:0051117 IPI
Biological Process
    ossification GO:0001503 IMP
    regulation of pH GO:0006885 IMP
    vacuolar acidification GO:0007035 IBA
    sensory perception of sound GO:0007605 IMP
    intracellular pH reduction GO:0051452 NAS
    renal tubular secretion GO:0097254 IMP
    synaptic vesicle lumen acidification GO:0097401 IEA
    proton transmembrane transport GO:1902600 IMP
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    vacuolar proton-transporting V-type ATPase, V0 domain GO:0000220 IEA
    lysosomal membrane GO:0005765 HDA
    endosome GO:0005768 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    endosome membrane GO:0010008 TAS
    basolateral plasma membrane GO:0016323 IEA
    apical plasma membrane GO:0016324 IDA
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IBA
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IDA
    phagocytic vesicle membrane GO:0030670 TAS
    synaptic vesicle membrane GO:0030672 IEA
    brush border membrane GO:0031526 IDA
    proton-transporting V-type ATPase complex GO:0033176 NAS
    apical part of cell GO:0045177 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ATP6V0A4 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
5
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP6V0A4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP1A2 477
Affinity Capture-MS Homo sapiens
2 CCDC115 84317
Affinity Capture-MS Homo sapiens
3 IGFN1  
Affinity Capture-MS Homo sapiens
4 ATP6V1D 51382
Affinity Capture-MS Homo sapiens
5 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
6 MYADM 91663
Affinity Capture-MS Homo sapiens
7 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
8 ATP6AP1 537
Affinity Capture-MS Homo sapiens
9 ASS1 445
Affinity Capture-MS Homo sapiens
10 ATP6V1C1 528
Affinity Capture-MS Homo sapiens
11 PFKM 5213
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 HYPK 25764
Affinity Capture-MS Homo sapiens
13 TMEM199  
Affinity Capture-MS Homo sapiens
14 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
15 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
16 FKBP5 2289
Affinity Capture-MS Homo sapiens
17 CUL9  
Affinity Capture-MS Homo sapiens
18 ATP6V0C 527
Affinity Capture-MS Homo sapiens
19 PCK1 5105
Affinity Capture-MS Homo sapiens
20 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
21 VMA21  
Affinity Capture-MS Homo sapiens
22 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
23 ATP6V1E1 529
Affinity Capture-MS Homo sapiens
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