Gene description for CLDN2
Gene name claudin 2
Gene symbol CLDN2
Other names/aliases -
Species Homo sapiens
 Database cross references - CLDN2
ExoCarta ExoCarta_9075
Vesiclepedia VP_9075
Entrez Gene 9075
HGNC 2041
MIM 300520
UniProt P57739  
 CLDN2 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
 Gene ontology annotations for CLDN2
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 ISS
    paracellular tight junction channel activity GO:0160187 IDA
Biological Process
    innate immune response in mucosa GO:0002227 ISS
    calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules GO:0016338 IBA
    calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules GO:0016338 ISS
    bicellular tight junction assembly GO:0070830 IBA
    cell-cell adhesion GO:0098609 ISS
    regulation of bile acid secretion GO:0120188 ISS
    paracellular transport GO:0160184 IDA
    regulation of intestinal D-glucose absorption GO:1903985 ISS
    regulation of intestinal lipid absorption GO:1904729 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    cell-cell junction GO:0005911 IDA
    bicellular tight junction GO:0005923 IBA
    bicellular tight junction GO:0005923 ISS
    tight junction GO:0070160 IDA
 Experiment description of studies that identified CLDN2 in sEVs
1
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for CLDN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOTCH2NL 388677
Two-hybrid Homo sapiens
2 LAT 27040
Two-hybrid Homo sapiens
3 CSGALNACT2 55454
Two-hybrid Homo sapiens
4 PLP1 5354
Two-hybrid Homo sapiens
5 KRTAP1-3  
Two-hybrid Homo sapiens
6 TMEM218  
Two-hybrid Homo sapiens
7 ADAM33 80332
Two-hybrid Homo sapiens
8 BTN3A2 11118
Two-hybrid Homo sapiens
9 TJP1 7082
Reconstituted Complex Homo sapiens
10 KRTAP1-1  
Two-hybrid Homo sapiens
11 IGFBP5 3488
Two-hybrid Homo sapiens
12 KRTAP10-3  
Two-hybrid Homo sapiens
13 EMP3 2014
Two-hybrid Homo sapiens
14 KRTAP4-12  
Two-hybrid Homo sapiens
15 FAM209A 200232
Two-hybrid Homo sapiens
16 UBE2I 7329
Two-hybrid Homo sapiens
17 TEX264 51368
Two-hybrid Homo sapiens
18 TJP2 9414
Two-hybrid Homo sapiens
19 CYSRT1 375791
Two-hybrid Homo sapiens
20 PIAS2  
Two-hybrid Homo sapiens
21 GRM2 2912
Two-hybrid Homo sapiens
22 IL27RA 9466
Two-hybrid Homo sapiens
23 CXCL9  
Two-hybrid Homo sapiens
24 KRT31 3881
Two-hybrid Homo sapiens
25 LPAR3  
Two-hybrid Homo sapiens
26 LNX1  
Two-hybrid Homo sapiens
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