Gene description for HYAL2
Gene name hyaluronoglucosaminidase 2
Gene symbol HYAL2
Other names/aliases LUCA2
Species Homo sapiens
 Database cross references - HYAL2
ExoCarta ExoCarta_8692
Vesiclepedia VP_8692
Entrez Gene 8692
HGNC 5321
MIM 603551
UniProt Q12891  
 HYAL2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for HYAL2
Molecular Function
    virus receptor activity GO:0001618 IDA
    transcription coactivator activity GO:0003713 IEA
    hyalurononglucosaminidase activity GO:0004415 IDA
    hyalurononglucosaminidase activity GO:0004415 IDA
    hyalurononglucosaminidase activity GO:0004415 TAS
    protein binding GO:0005515 IPI
    hyaluronic acid binding GO:0005540 IDA
    enzyme binding GO:0019899 IPI
    receptor signaling protein tyrosine kinase inhibitor activity GO:0030294 IDA
    receptor tyrosine kinase binding GO:0030971 IPI
    hyaluronoglucuronidase activity GO:0033906 IBA
    hyaluronoglucuronidase activity GO:0033906 IDA
    transforming growth factor beta binding GO:0050431 ISS
Biological Process
    response to reactive oxygen species GO:0000302 IDA
    kidney development GO:0001822 ISS
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    carbohydrate metabolic process GO:0005975 IEA
    glycosaminoglycan catabolic process GO:0006027 IDA
    response to virus GO:0009615 IDA
    negative regulation of fibroblast migration GO:0010764 IDA
    fusion of virus membrane with host plasma membrane GO:0019064 IDA
    hyaluronan catabolic process GO:0030214 IBA
    hyaluronan catabolic process GO:0030214 IDA
    hyaluronan catabolic process GO:0030214 TAS
    negative regulation of cell growth GO:0030308 IDA
    positive regulation of interleukin-6 production GO:0032755 IDA
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of urine volume GO:0035810 ISS
    monocyte activation GO:0042117 IDA
    positive regulation of protein import into nucleus GO:0042307 ISS
    negative regulation of MAP kinase activity GO:0043407 IDA
    cellular response to fibroblast growth factor stimulus GO:0044344 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    response to antibiotic GO:0046677 IEP
    symbiont entry into host cell GO:0046718 ISS
    skeletal system morphogenesis GO:0048705 IEA
    positive regulation of inflammatory response GO:0050729 IDA
    cartilage development GO:0051216 IEP
    defense response to virus GO:0051607 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IDA
    multicellular organismal-level iron ion homeostasis GO:0060586 IEA
    negative regulation of protein tyrosine kinase activity GO:0061099 IDA
    renal water absorption GO:0070295 ISS
    cellular response to interleukin-1 GO:0071347 IDA
    cellular response to tumor necrosis factor GO:0071356 IEP
    cellular response to UV-B GO:0071493 IDA
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 ISS
Subcellular Localization
    Golgi membrane GO:0000139 ISS
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IDA
    endoplasmic reticulum GO:0005783 NAS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    microvillus GO:0005902 IDA
    external side of plasma membrane GO:0009897 IDA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISS
    apical plasma membrane GO:0016324 IDA
    endocytic vesicle GO:0030139 IDA
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 IDA
    membrane raft GO:0045121 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    RNA polymerase II transcription regulator complex GO:0090575 ISS
 Experiment description of studies that identified HYAL2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HYAL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
3 C7orf34  
Affinity Capture-MS Homo sapiens
4 GALNT10 55568
Affinity Capture-MS Homo sapiens
5 BTNL2  
Affinity Capture-MS Homo sapiens
6 TMEM106A  
Affinity Capture-MS Homo sapiens
7 KLK15  
Affinity Capture-MS Homo sapiens
8 CEACAM8 1088
Affinity Capture-MS Homo sapiens
9 LGI1  
Affinity Capture-MS Homo sapiens
10 CLGN 1047
Affinity Capture-MS Homo sapiens
11 HLA-G 3135
Affinity Capture-MS Homo sapiens
12 SFTPC  
Affinity Capture-MS Homo sapiens
13 FSTL4  
Affinity Capture-MS Homo sapiens
14 DCT 1638
Affinity Capture-MS Homo sapiens
15 B3GNT3 10331
Affinity Capture-MS Homo sapiens
16 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TMEM25  
Affinity Capture-MS Homo sapiens
18 SLC39A5 283375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GGH 8836
Affinity Capture-MS Homo sapiens
20 PON2 5445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ASIC4  
Affinity Capture-MS Homo sapiens
22 PTCH1  
Affinity Capture-MS Homo sapiens
23 DPEP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PDGFRA 5156
Affinity Capture-MS Homo sapiens
25 PRG2  
Affinity Capture-MS Homo sapiens
26 MFAP4 4239
Affinity Capture-MS Homo sapiens
27 ECEL1  
Affinity Capture-MS Homo sapiens
28 ALPP 250
Affinity Capture-MS Homo sapiens
29 FHL3 2275
Two-hybrid Homo sapiens
30 B4GAT1 11041
Affinity Capture-MS Homo sapiens
31 DPEP1 1800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 GAA 2548
Affinity Capture-MS Homo sapiens
33 KRTAP3-2  
Two-hybrid Homo sapiens
34 TSPAN18 90139
Affinity Capture-MS Homo sapiens
35 KRTAP10-3  
Two-hybrid Homo sapiens
36 SLURP1  
Affinity Capture-MS Homo sapiens
37 TAS1R3  
Affinity Capture-MS Homo sapiens
38 TRIM54  
Two-hybrid Homo sapiens
39 FUT3 2525
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ALPI  
Affinity Capture-MS Homo sapiens
41 POLH  
Affinity Capture-MS Homo sapiens
42 EMR4P  
Affinity Capture-MS Homo sapiens
43 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
44 C1QB 713
Affinity Capture-MS Homo sapiens
45 BRICD5  
Affinity Capture-MS Homo sapiens
46 NXPH3  
Affinity Capture-MS Homo sapiens
47 SDF2L1 23753
Affinity Capture-MS Homo sapiens
48 CLEC12B  
Affinity Capture-MS Homo sapiens
49 SCGB2A2  
Affinity Capture-MS Homo sapiens
50 C1orf54  
Affinity Capture-MS Homo sapiens
51 PSCA 8000
Affinity Capture-MS Homo sapiens
52 NCEH1 57552
Affinity Capture-MS Homo sapiens
53 DHH  
Affinity Capture-MS Homo sapiens
54 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PRSS8 5652
Affinity Capture-MS Homo sapiens
56 GXYLT1 283464
Affinity Capture-MS Homo sapiens
57 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 LYPD1  
Affinity Capture-MS Homo sapiens
59 CLEC2B  
Affinity Capture-MS Homo sapiens
60 B3GAT3 26229
Affinity Capture-MS Homo sapiens
61 MPPE1 65258
Affinity Capture-MS Homo sapiens
62 KLRC1  
Affinity Capture-MS Homo sapiens
63 IL5RA  
Affinity Capture-MS Homo sapiens
64 CFC1  
Affinity Capture-MS Homo sapiens
65 LYPD4  
Affinity Capture-MS Homo sapiens
66 LIPG 9388
Affinity Capture-MS Homo sapiens
67 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
68 TAS1R2  
Affinity Capture-MS Homo sapiens
69 IGFL3  
Affinity Capture-MS Homo sapiens
70 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here