Experiment description of studies that identified METRNL in sEVs

1 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

2 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 66 MISEV standards ✔ IEM Biophysical techniques ✔ TSG101|Alix|CD63|CD81 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

Protein-protein interactions for METRNL

Protein Interactor ExoCarta ID Identification method PubMed Species 1 LY86   Affinity Capture-MS Homo sapiens 2 CCL3   Affinity Capture-MS Homo sapiens 3 FMOD 2331 Affinity Capture-MS Homo sapiens 4 CA6 765 Affinity Capture-MS Homo sapiens 5 EPDR1 54749 Affinity Capture-MS Homo sapiens 6 BCAN   Affinity Capture-MS Homo sapiens 7 PRG3   Affinity Capture-MS Homo sapiens 8 TMEM87A 25963 Affinity Capture-MS Homo sapiens 9 TCTN1   Affinity Capture-MS Homo sapiens 10 FSTL4   Affinity Capture-MS Homo sapiens 11 TMEM25   Affinity Capture-MS Homo sapiens 12 EFNA2   Affinity Capture-MS Homo sapiens 13 TRGV3   Affinity Capture-MS Homo sapiens 14 ASIC4   Affinity Capture-MS Homo sapiens 15 CFC1   Affinity Capture-MS Homo sapiens 16 BTLA   Affinity Capture-MS Homo sapiens 17 TM2D3   Affinity Capture-MS Homo sapiens 18 PRG2   Affinity Capture-MS Homo sapiens 19 MFAP4 4239 Affinity Capture-MS Homo sapiens 20 PI15   Affinity Capture-MS Homo sapiens 21 CBLN4   Affinity Capture-MS Homo sapiens 22 OIT3 170392 Affinity Capture-MS Homo sapiens 23 KLRD1   Affinity Capture-MS Homo sapiens 24 LYZL1   Affinity Capture-MS Homo sapiens 25 SLURP1   Affinity Capture-MS Homo sapiens 26 NCR3   Affinity Capture-MS Homo sapiens 27 DDX58 23586 Affinity Capture-RNA Homo sapiens 28 A2M 2 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 ELSPBP1   Affinity Capture-MS Homo sapiens 30 SFTPC   Affinity Capture-MS Homo sapiens 31 EPHA7 2045 Affinity Capture-MS Homo sapiens 32 MSTN   Affinity Capture-MS Homo sapiens 33 BTNL2   Affinity Capture-MS Homo sapiens 34 GAA 2548 Affinity Capture-MS Homo sapiens 35 C1QB 713 Affinity Capture-MS Homo sapiens 36 SIRPD   Affinity Capture-MS Homo sapiens 37 BRICD5   Affinity Capture-MS Homo sapiens 38 CHMP4B 128866 Affinity Capture-MS Homo sapiens 39 CST11   Affinity Capture-MS Homo sapiens 40 CRISP2   Affinity Capture-MS Homo sapiens 41 GGH 8836 Affinity Capture-MS Homo sapiens 42 DEFA5   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 ALPP 250 Affinity Capture-MS Homo sapiens 44 DNAJB9 4189 Affinity Capture-MS Homo sapiens 45 NAGA 4668 Affinity Capture-MS Homo sapiens 46 IDS   Affinity Capture-MS Homo sapiens 47 CDHR4   Affinity Capture-MS Homo sapiens 48 ADIPOQ 9370 Affinity Capture-MS Homo sapiens 49 DNASE1L1 1774 Affinity Capture-MS Homo sapiens 50 TDGF1   Affinity Capture-MS Homo sapiens 51 IL5RA   Affinity Capture-MS Homo sapiens 52 CST8   Affinity Capture-MS Homo sapiens 53 SDF2L1 23753 Affinity Capture-MS Homo sapiens 54 LIPG 9388 Affinity Capture-MS Homo sapiens View the network

Pathways in which METRNL is involved

No pathways found