Gene description for MAN2B2
Gene name mannosidase, alpha, class 2B, member 2
Gene symbol MAN2B2
Other names/aliases -
Species Homo sapiens
 Database cross references - MAN2B2
ExoCarta ExoCarta_23324
Vesiclepedia VP_23324
Entrez Gene 23324
HGNC 29623
UniProt Q9Y2E5  
 MAN2B2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MAN2B2
Molecular Function
    alpha-mannosidase activity GO:0004559 IBA
    protein binding GO:0005515 IPI
    carbohydrate binding GO:0030246 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    mannose metabolic process GO:0006013 IEA
    oligosaccharide catabolic process GO:0009313 IEA
Subcellular Localization
    lysosome GO:0005764 IBA
    lysosomal lumen GO:0043202 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MAN2B2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAN2B2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
3 MANEA 79694
Affinity Capture-MS Homo sapiens
4 FCGR3B  
Affinity Capture-MS Homo sapiens
5 POM121L1P  
Affinity Capture-MS Homo sapiens
6 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 KLK15  
Affinity Capture-MS Homo sapiens
8 C1QTNF9B  
Affinity Capture-MS Homo sapiens
9 CLGN 1047
Affinity Capture-MS Homo sapiens
10 SIAE 54414
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TMEM87A 25963
Affinity Capture-MS Homo sapiens
12 DPEP2  
Affinity Capture-MS Homo sapiens
13 LCN6  
Affinity Capture-MS Homo sapiens
14 TCTN1  
Affinity Capture-MS Homo sapiens
15 FSTL4  
Affinity Capture-MS Homo sapiens
16 UQCC1 55245
Affinity Capture-MS Homo sapiens
17 IGLL5 100423062
Affinity Capture-MS Homo sapiens
18 TSHR 7253
Affinity Capture-MS Homo sapiens
19 GGH 8836
Affinity Capture-MS Homo sapiens
20 FUCA2 2519
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TRGV3  
Affinity Capture-MS Homo sapiens
22 WDR25  
Affinity Capture-MS Homo sapiens
23 TM2D3  
Affinity Capture-MS Homo sapiens
24 PRG2  
Affinity Capture-MS Homo sapiens
25 MFAP4 4239
Affinity Capture-MS Homo sapiens
26 PI15  
Affinity Capture-MS Homo sapiens
27 CBLN4  
Affinity Capture-MS Homo sapiens
28 C4orf26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 IGF2R 3482
Affinity Capture-MS Homo sapiens
30 LYZL1  
Affinity Capture-MS Homo sapiens
31 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PRR14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SLURP1  
Affinity Capture-MS Homo sapiens
34 NCR3  
Affinity Capture-MS Homo sapiens
35 IL5RA  
Affinity Capture-MS Homo sapiens
36 KLK2  
Affinity Capture-MS Homo sapiens
37 SPANXN4  
Affinity Capture-MS Homo sapiens
38 CCDC74A  
Affinity Capture-MS Homo sapiens
39 C1QB 713
Affinity Capture-MS Homo sapiens
40 SIRPD  
Affinity Capture-MS Homo sapiens
41 IDS  
Affinity Capture-MS Homo sapiens
42 CSN1S1 1446
Affinity Capture-MS Homo sapiens
43 SDF2L1 23753
Affinity Capture-MS Homo sapiens
44 FIBIN  
Affinity Capture-MS Homo sapiens
45 FBXO2 26232
Affinity Capture-MS Homo sapiens
46 CRISP2  
Affinity Capture-MS Homo sapiens
47 C1orf54  
Affinity Capture-MS Homo sapiens
48 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
49 ALPP 250
Affinity Capture-MS Homo sapiens
50 DNAJB9 4189
Affinity Capture-MS Homo sapiens
51 TCF7  
Affinity Capture-MS Homo sapiens
52 CELA2B  
Affinity Capture-MS Homo sapiens
53 EDN3  
Affinity Capture-MS Homo sapiens
54 NXPH3  
Affinity Capture-MS Homo sapiens
55 SPN 6693
Affinity Capture-MS Homo sapiens
56 DUX3  
Affinity Capture-MS Homo sapiens
57 LYPD4  
Affinity Capture-MS Homo sapiens
58 DDX28  
Affinity Capture-MS Homo sapiens
59 LIPG 9388
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MAN2B2 is involved
PathwayEvidenceSource
Lysosomal oligosaccharide catabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome





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