Gene description for CSPG4
Gene name chondroitin sulfate proteoglycan 4
Gene symbol CSPG4
Other names/aliases HMW-MAA
MCSP
MCSPG
MEL-CSPG
MSK16
NG2
Species Homo sapiens
 Database cross references - CSPG4
ExoCarta ExoCarta_1464
Entrez Gene 1464
HGNC 2466
MIM 601172
UniProt Q6UVK1  
 CSPG4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CSPG4
Molecular Function
    protein kinase binding GO:0019901 IPI
    signal transducer activity GO:0004871 IEA
Biological Process
    intracellular signal transduction GO:0035556 IDA
    glycosaminoglycan metabolic process GO:0030203 TAS
    cell proliferation GO:0008283 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    carbohydrate metabolic process GO:0005975 TAS
    small molecule metabolic process GO:0044281 TAS
    tissue remodeling GO:0048771 IEA
    glial cell migration GO:0008347 IEA
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IEA
    angiogenesis GO:0001525 IEA
    activation of MAPK activity GO:0000187 IEA
    chondroitin sulfate catabolic process GO:0030207 TAS
    chondroitin sulfate metabolic process GO:0030204 TAS
    chondroitin sulfate biosynthetic process GO:0030206 TAS
    dermatan sulfate biosynthetic process GO:0030208 TAS
Subcellular Localization
    Golgi lumen GO:0005796 TAS
    lamellipodium membrane GO:0031258 IEA
    focal adhesion GO:0005925 IDA
    lysosomal lumen GO:0043202 TAS
    integral component of plasma membrane GO:0005887 TAS
    cell surface GO:0009986 TAS
    extracellular region GO:0005576 TAS
    apical plasma membrane GO:0016324 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CSPG4 in exosomes
1
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CSPG4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRIP2 80852
Two-hybrid Homo sapiens
2 CDC42 998
Affinity Capture-Western Homo sapiens
3 MPDZ  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
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