Gene description for CTU1
Gene name cytosolic thiouridylase subunit 1
Gene symbol CTU1
Other names/aliases ATPBD3
NCS6
Species Homo sapiens
 Database cross references - CTU1
ExoCarta ExoCarta_90353
Vesiclepedia VP_90353
Entrez Gene 90353
HGNC 29590
MIM 612694
UniProt Q7Z7A3  
 CTU1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CTU1
Molecular Function
    tRNA binding GO:0000049 IBA
    protein binding GO:0005515 IPI
    nucleotidyltransferase activity GO:0016779 IEA
Biological Process
    tRNA wobble uridine modification GO:0002098 NAS
    tRNA wobble position uridine thiolation GO:0002143 IBA
    protein urmylation GO:0032447 IEA
    tRNA thio-modification GO:0034227 NAS
Subcellular Localization
    cytosolic tRNA wobble base thiouridylase complex GO:0002144 IBA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CTU1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CTU1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NYX  
Affinity Capture-MS Homo sapiens
2 CETN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 VWCE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 EDAR  
Affinity Capture-MS Homo sapiens
5 FHL5 9457
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GAN 8139
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 SCRN1 9805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 GRN 2896
Affinity Capture-MS Homo sapiens
10 SLC31A1 1317
Affinity Capture-MS Homo sapiens
11 FKBPL  
Affinity Capture-MS Homo sapiens
12 AIFM1 9131
Affinity Capture-MS Homo sapiens
13 SPHK1 8877
Affinity Capture-MS Homo sapiens
14 THUMPD3 25917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CIAO1 9391
Affinity Capture-MS Homo sapiens
16 ZNF444  
Affinity Capture-MS Homo sapiens
17 LDLRAD1  
Affinity Capture-MS Homo sapiens
18 LTBR 4055
Affinity Capture-MS Homo sapiens
19 ARSA 410
Affinity Capture-MS Homo sapiens
20 IL25  
Affinity Capture-MS Homo sapiens
21 P4HA2 8974
Affinity Capture-MS Homo sapiens
22 WDR92 116143
Affinity Capture-MS Homo sapiens
23 RP2 6102
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 S100A2 6273
Affinity Capture-MS Homo sapiens
25 MAGEA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SPINT2 10653
Affinity Capture-MS Homo sapiens
27 CUL3 8452
Affinity Capture-MS Homo sapiens
28 PRAMEF9  
Affinity Capture-MS Homo sapiens
29 LOXL3 84695
Affinity Capture-MS Homo sapiens
30 ECHDC2 55268
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 EGFR 1956
Negative Genetic Homo sapiens
32 SCPEP1 59342
Affinity Capture-MS Homo sapiens
33 CTU2 348180
Affinity Capture-MS Homo sapiens
34 FAM63B 54629
Affinity Capture-MS Homo sapiens
35 SLURP1  
Affinity Capture-MS Homo sapiens
36 FAXC  
Affinity Capture-MS Homo sapiens
37 TINAG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 VASP 7408
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 FAM96B 51647
Affinity Capture-MS Homo sapiens
40 MFAP5  
Affinity Capture-MS Homo sapiens
41 ZNF414  
Affinity Capture-MS Homo sapiens
42 CHCHD4  
Affinity Capture-MS Homo sapiens
43 HS1BP3 64342
Affinity Capture-MS Homo sapiens
44 HHIPL1 84439
Affinity Capture-MS Homo sapiens
45 PBXIP1 57326
Affinity Capture-MS Homo sapiens
46 CCDC107  
Affinity Capture-MS Homo sapiens
47 FBXO2 26232
Affinity Capture-MS Homo sapiens
48 RAB24 53917
Affinity Capture-MS Homo sapiens
49 ACBD6 84320
Affinity Capture-MS Homo sapiens
50 SNX11 29916
Affinity Capture-MS Homo sapiens
51 RAB40C 57799
Affinity Capture-MS Homo sapiens
52 CCNJL  
Affinity Capture-MS Homo sapiens
53 GPSM3  
Affinity Capture-MS Homo sapiens
54 HSPA4 3308
Affinity Capture-MS Homo sapiens
55 KCTD17 79734
Affinity Capture-MS Homo sapiens
56 S100B 6285
Affinity Capture-MS Homo sapiens
57 FLII 2314
Affinity Capture-MS Homo sapiens
58 SSSCA1 10534
Affinity Capture-MS Homo sapiens
59 ECM1 1893
Affinity Capture-MS Homo sapiens
60 DCAF4  
Affinity Capture-MS Homo sapiens
61 AKAP7 9465
Affinity Capture-MS Homo sapiens
62 LBHD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 UROS 7390
Affinity Capture-MS Homo sapiens
64 CHIT1  
Affinity Capture-MS Homo sapiens
65 MYCL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 KRAS 3845
Negative Genetic Homo sapiens
67 GIP  
Affinity Capture-MS Homo sapiens
68 URM1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
69 PLA2G10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 RAP2A 5911
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CTU1 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





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