Gene description for TPD52L1
Gene name tumor protein D52-like 1
Gene symbol TPD52L1
Other names/aliases D53
hD53
Species Homo sapiens
 Database cross references - TPD52L1
ExoCarta ExoCarta_7164
Vesiclepedia VP_7164
Entrez Gene 7164
HGNC 12006
MIM 604069
UniProt Q16890  
 TPD52L1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TPD52L1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 IBA
    G2/M transition of mitotic cell cycle GO:0000086 IDA
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of JNK cascade GO:0046330 IBA
    positive regulation of JNK cascade GO:0046330 IDA
    positive regulation of apoptotic signaling pathway GO:2001235 IBA
    positive regulation of apoptotic signaling pathway GO:2001235 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified TPD52L1 in exosomes
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TPD52L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tpd52  
Two-hybrid Mus musculus
2 ABL2  
Two-hybrid Homo sapiens
3 TNFRSF1B  
Affinity Capture-MS Homo sapiens
4 PSMC3 5702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GRIN3B  
Affinity Capture-MS Homo sapiens
6 LAMTOR1 55004
Proximity Label-MS Homo sapiens
7 NPM3 10360
Co-fractionation Homo sapiens
8 YWHAB 7529
Affinity Capture-MS Homo sapiens
9 TPD52L1 7164
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 KCTD12 115207
Affinity Capture-MS Homo sapiens
11 S100A2 6273
Co-fractionation Homo sapiens
12 MAL2 114569
Two-hybrid Homo sapiens
13 AZIN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 AGMAT  
Affinity Capture-MS Homo sapiens
15 TPD52L2 7165
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
16 GSKIP  
Affinity Capture-MS Homo sapiens
17 HDAC1 3065
Two-hybrid Homo sapiens
18 UCHL3 7347
Co-fractionation Homo sapiens
19 PBX3  
Two-hybrid Homo sapiens
20 RNF40 9810
Co-fractionation Homo sapiens
21 TPD52 7163
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
22 UBR5 51366
Co-fractionation Homo sapiens
23 EIF3J 8669
Two-hybrid Homo sapiens
24 TPD52L3  
Affinity Capture-MS Homo sapiens
25 ISYNA1 51477
Co-fractionation Homo sapiens
26 EPN3 55040
Affinity Capture-MS Homo sapiens
27 GABBR1  
Affinity Capture-MS Homo sapiens
28 GOPC 57120
Affinity Capture-MS Homo sapiens
29 YWHAQ 10971
Affinity Capture-MS Homo sapiens
30 GPR135  
Two-hybrid Homo sapiens
31 YAE1D1  
Two-hybrid Homo sapiens
32 TXNL4A 10907
Affinity Capture-MS Homo sapiens
33 PAK6 56924
Two-hybrid Homo sapiens
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