Gene description for GNG2
Gene name guanine nucleotide binding protein (G protein), gamma 2
Gene symbol GNG2
Other names/aliases -
Species Homo sapiens
 Database cross references - GNG2
ExoCarta ExoCarta_54331
Entrez Gene 54331
HGNC 4404
MIM 606981
UniProt P59768  
 GNG2 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for GNG2
Molecular Function
    signal transducer activity GO:0004871 IEA
    G-protein beta-subunit binding GO:0031681 IPI
Biological Process
    cellular response to glucagon stimulus GO:0071377 TAS
    cell proliferation GO:0008283 IEA
    energy reserve metabolic process GO:0006112 TAS
    blood coagulation GO:0007596 TAS
    small molecule metabolic process GO:0044281 TAS
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 ISS
    platelet activation GO:0030168 TAS
    cellular response to catecholamine stimulus GO:0071870 ISS
    synaptic transmission GO:0007268 TAS
    cellular response to prostaglandin E stimulus GO:0071380 ISS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 TAS
    heterotrimeric G-protein complex GO:0005834 ISS
    membrane GO:0016020 ISS
 Experiment description of studies that identified GNG2 in exosomes
1
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNB5 10681
Two-hybrid Homo sapiens
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