Gene description for NLN
Gene name neurolysin (metallopeptidase M3 family)
Gene symbol NLN
Other names/aliases AGTBP
EP24.16
MEP
MOP
Species Homo sapiens
 Database cross references - NLN
ExoCarta ExoCarta_57486
Vesiclepedia VP_57486
Entrez Gene 57486
HGNC 16058
MIM 611530
UniProt Q9BYT8  
 NLN identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NLN
Molecular Function
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 TAS
    peptide binding GO:0042277 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    regulation of gluconeogenesis GO:0006111 IEA
    proteolysis GO:0006508 IBA
    peptide metabolic process GO:0006518 IBA
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    regulation of skeletal muscle fiber differentiation GO:1902809 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    mitochondrion GO:0005739 HTP
    mitochondrial intermembrane space GO:0005758 IBA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified NLN in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NLN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Naa11  
Affinity Capture-MS Mus musculus
2 GPRIN3  
Affinity Capture-MS Homo sapiens
3 RASSF8 11228
Affinity Capture-MS Homo sapiens
4 KIF14 9928
Affinity Capture-MS Homo sapiens
5 GAR1 54433
Affinity Capture-MS Homo sapiens
6 KRTAP19-6  
Affinity Capture-MS Homo sapiens
7 Calml3  
Affinity Capture-MS Mus musculus
8 MRPL9 65005
Affinity Capture-MS Homo sapiens
9 ELP3 55140
Affinity Capture-MS Homo sapiens
10 PDHA1 5160
Affinity Capture-MS Homo sapiens
11 KIF20A 10112
Affinity Capture-MS Homo sapiens
12 RLN1  
Affinity Capture-MS Homo sapiens
13 SYNE1 23345
Co-fractionation Homo sapiens
14 RUFY1 80230
Affinity Capture-MS Homo sapiens
15 ZNF526  
Affinity Capture-MS Homo sapiens
16 SPACA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 KLC4 89953
Affinity Capture-MS Homo sapiens
18 APP 351
Reconstituted Complex Homo sapiens
19 KRTAP19-7  
Affinity Capture-MS Homo sapiens
20 Dicer1  
Affinity Capture-MS Mus musculus
21 SMTNL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 NCAPD3 23310
Affinity Capture-MS Homo sapiens
23 Cetn2  
Affinity Capture-MS Mus musculus
24 PARK2  
Affinity Capture-MS Homo sapiens
25 DLST 1743
Affinity Capture-MS Homo sapiens
26 KRTAP19-4  
Affinity Capture-MS Homo sapiens
27 CIT 11113
Affinity Capture-MS Homo sapiens
28 LDLRAP1 26119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FPGT 8790
Co-fractionation Homo sapiens
30 LMLN  
Affinity Capture-MS Homo sapiens
31 KRTAP6-1  
Affinity Capture-MS Homo sapiens
32 Tnks2  
Affinity Capture-MS Mus musculus
33 PGAM1 5223
Co-fractionation Homo sapiens
34 Fign  
Affinity Capture-MS Mus musculus
35 CUL4A 8451
Affinity Capture-MS Homo sapiens
36 RPA3 6119
Proximity Label-MS Homo sapiens
37 Eea1  
Affinity Capture-MS Mus musculus
38 DYNLL1 8655
Affinity Capture-MS Homo sapiens
39 MBP 4155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RGS20 8601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 HIF1A 3091
Affinity Capture-MS Homo sapiens
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