Gene description for B3GNT3
Gene name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Gene symbol B3GNT3
Other names/aliases B3GAL-T8
B3GN-T3
B3GNT-3
HP10328
TMEM3
beta3Gn-T3
Species Homo sapiens
 Database cross references - B3GNT3
ExoCarta ExoCarta_10331
Vesiclepedia VP_10331
Entrez Gene 10331
HGNC 13528
MIM 605863
UniProt Q9Y2A9  
 B3GNT3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
 Gene ontology annotations for B3GNT3
Molecular Function
    N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity GO:0008499 TAS
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity GO:0008532 IBA
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity GO:0008532 IDA
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity GO:0008532 TAS
    beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity GO:0047223 IEA
Biological Process
    protein O-linked glycosylation GO:0006493 IBA
    O-glycan processing GO:0016266 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    poly-N-acetyllactosamine biosynthetic process GO:0030311 IBA
    poly-N-acetyllactosamine biosynthetic process GO:0030311 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified B3GNT3 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for B3GNT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADAM10 102
Affinity Capture-MS Homo sapiens
2 NRG1 3084
Affinity Capture-MS Homo sapiens
3 NGLY1 55768
Affinity Capture-MS Homo sapiens
4 MBOAT7 79143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HFE  
Affinity Capture-MS Homo sapiens
6 ACSL1 2180
Affinity Capture-MS Homo sapiens
7 TUBA1A 7846
Affinity Capture-MS Homo sapiens
8 PRPS1L1 221823
Affinity Capture-MS Homo sapiens
9 CHPF 79586
Affinity Capture-MS Homo sapiens
10 HSPA5 3309
Affinity Capture-MS Homo sapiens
11 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
12 GPAA1 8733
Affinity Capture-MS Homo sapiens
13 SPG7  
Affinity Capture-MS Homo sapiens
14 CD19 930
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ADCK3  
Affinity Capture-MS Homo sapiens
17 HLA-G 3135
Affinity Capture-MS Homo sapiens
18 PGRMC1 10857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GALNT1 2589
Affinity Capture-MS Homo sapiens
20 ATP1A3 478
Affinity Capture-MS Homo sapiens
21 CHID1 66005
Affinity Capture-MS Homo sapiens
22 PDIA4 9601
Affinity Capture-MS Homo sapiens
23 KDELC1 79070
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 DSTYK 25778
Affinity Capture-MS Homo sapiens
25 TAP1 6890
Affinity Capture-MS Homo sapiens
26 PON2 5445
Affinity Capture-MS Homo sapiens
27 PDGFRA 5156
Affinity Capture-MS Homo sapiens
28 TUBB8 347688
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 NOMO1 23420
Affinity Capture-MS Homo sapiens
30 LRFN3  
Affinity Capture-MS Homo sapiens
31 ERLEC1 27248
Affinity Capture-MS Homo sapiens
32 PGRMC2 10424
Affinity Capture-MS Homo sapiens
33 PTDSS2 81490
Affinity Capture-MS Homo sapiens
34 PIGK 10026
Affinity Capture-MS Homo sapiens
35 CNPY3 10695
Affinity Capture-MS Homo sapiens
36 PIGU 128869
Affinity Capture-MS Homo sapiens
37 FBXO6 26270
Affinity Capture-MS Homo sapiens
38 DCAF11  
Affinity Capture-MS Homo sapiens
39 DCAF6 55827
Affinity Capture-MS Homo sapiens
40 SLURP1  
Affinity Capture-MS Homo sapiens
41 OS9 10956
Affinity Capture-MS Homo sapiens
42 GHITM 27069
Affinity Capture-MS Homo sapiens
43 MYO19  
Affinity Capture-MS Homo sapiens
44 HS6ST1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 GALNT12 79695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SFTPC  
Affinity Capture-MS Homo sapiens
47 AMIGO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CHPF2 54480
Affinity Capture-MS Homo sapiens
49 EGFR 1956
Affinity Capture-MS Homo sapiens
50 ITGAV 3685
Affinity Capture-MS Homo sapiens
51 COA7  
Affinity Capture-MS Homo sapiens
52 SEPN1 57190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 C1QB 713
Affinity Capture-MS Homo sapiens
54 FUT11 170384
Affinity Capture-MS Homo sapiens
55 B3GNT2 10678
Affinity Capture-MS Homo sapiens
56 TMEM132A 54972
Affinity Capture-MS Homo sapiens
57 SDF2L1 23753
Affinity Capture-MS Homo sapiens
58 CRLF1 9244
Affinity Capture-MS Homo sapiens
59 FBXO2 26232
Affinity Capture-MS Homo sapiens
60 CLSTN3 9746
Affinity Capture-MS Homo sapiens
61 FECH 2235
Affinity Capture-MS Homo sapiens
62 IRAK1  
Affinity Capture-MS Homo sapiens
63 SLC30A7 148867
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 C6orf120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 TMEM201 199953
Affinity Capture-MS Homo sapiens
66 GALNT7 51809
Affinity Capture-MS Homo sapiens
67 HYAL2 8692
Affinity Capture-MS Homo sapiens
68 CLEC2B  
Affinity Capture-MS Homo sapiens
69 SPATA5 166378
Affinity Capture-MS Homo sapiens
70 ERGIC1 57222
Affinity Capture-MS Homo sapiens
71 PCYOX1L 78991
Affinity Capture-MS Homo sapiens
72 SUMF1 285362
Affinity Capture-MS Homo sapiens
73 CLGN 1047
Affinity Capture-MS Homo sapiens
74 HLA-C 3107
Affinity Capture-MS Homo sapiens
75 TMEM131  
Affinity Capture-MS Homo sapiens
76 CBWD1 55871
Affinity Capture-MS Homo sapiens
77 SLC30A6 55676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 PIGS 94005
Affinity Capture-MS Homo sapiens
79 TAP2 6891
Affinity Capture-MS Homo sapiens
80 SLC30A5 64924
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 RMDN3 55177
Affinity Capture-MS Homo sapiens
82 LMAN2 10960
Affinity Capture-MS Homo sapiens
83 CLN6  
Affinity Capture-MS Homo sapiens
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