Gene description for ABCC2
Gene name ATP-binding cassette, sub-family C (CFTR/MRP), member 2
Gene symbol ABCC2
Other names/aliases ABC30
CMOAT
DJS
MRP2
cMRP
Species Homo sapiens
 Database cross references - ABCC2
ExoCarta ExoCarta_1244
Vesiclepedia VP_1244
Entrez Gene 1244
HGNC 53
MIM 601107
UniProt Q92887  
 ABCC2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
 Gene ontology annotations for ABCC2
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    organic anion transmembrane transporter activity GO:0008514 TAS
    ABC-type xenobiotic transporter activity GO:0008559 IMP
    ABC-type xenobiotic transporter activity GO:0008559 TAS
    bilirubin transmembrane transporter activity GO:0015127 IMP
    bilirubin transmembrane transporter activity GO:0015127 TAS
    ABC-type glutathione S-conjugate transporter activity GO:0015431 IMP
    ATP hydrolysis activity GO:0016887 IEA
    ATPase-coupled transmembrane transporter activity GO:0042626 IBA
    ATPase-coupled transmembrane transporter activity GO:0042626 IDA
    xenobiotic transmembrane transporter activity GO:0042910 IDA
    ATPase-coupled inorganic anion transmembrane transporter activity GO:0043225 TAS
    ABC-type transporter activity GO:0140359 TAS
Biological Process
    xenobiotic metabolic process GO:0006805 TAS
    xenobiotic transmembrane transport GO:0006855 IMP
    negative regulation of gene expression GO:0010629 ISS
    bile acid and bile salt transport GO:0015721 IMP
    bilirubin transport GO:0015723 IMP
    heme catabolic process GO:0042167 TAS
    xenobiotic export from cell GO:0046618 IMP
    transmembrane transport GO:0055085 IBA
    transmembrane transport GO:0055085 TAS
    transepithelial transport GO:0070633 ISS
    leukotriene transport GO:0071716 IMP
    monoatomic anion transmembrane transport GO:0098656 IEA
    transport across blood-brain barrier GO:0150104 NAS
    xenobiotic transport across blood-brain barrier GO:1990962 ISS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 HDA
    cell surface GO:0009986 IDA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    intercellular canaliculus GO:0046581 IEA
 Experiment description of studies that identified ABCC2 in exosomes
1
Experiment ID 283
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 285
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 286
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ABCC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OXA1L  
Co-fractionation Homo sapiens
2 CYP26B1  
Affinity Capture-MS Homo sapiens
3 MRPL57  
Co-fractionation Homo sapiens
4 LGALS9 3965
Affinity Capture-MS Homo sapiens
5 UQCRFS1 7386
Co-fractionation Homo sapiens
6 SHANK3 85358
Two-hybrid Homo sapiens
7 EEF1A1 1915
Co-fractionation Homo sapiens
8 JUP 3728
Co-fractionation Homo sapiens
9 TOMM22 56993
Co-fractionation Homo sapiens
10 MRPS35 60488
Co-fractionation Homo sapiens
11 OPALIN  
Affinity Capture-MS Homo sapiens
12 MRPS28  
Co-fractionation Homo sapiens
13 ZDHHC12  
Affinity Capture-MS Homo sapiens
14 MRPL10 124995
Co-fractionation Homo sapiens
15 LGALS3 3958
Affinity Capture-MS Homo sapiens
16 NDUFA9 4704
Co-fractionation Homo sapiens
17 MRPL24  
Co-fractionation Homo sapiens
18 MRPL37 51253
Co-fractionation Homo sapiens
19 TMEM9B 56674
Affinity Capture-MS Homo sapiens
20 B4GAT1 11041
Affinity Capture-MS Homo sapiens
21 EIF4A1 1973
Co-fractionation Homo sapiens
22 VAMP2 6844
Co-fractionation Homo sapiens
23 RAB14 51552
Co-fractionation Homo sapiens
24 AZIN1 51582
Two-hybrid Homo sapiens
25 PRR3  
Co-fractionation Homo sapiens
26 FFAR1  
Affinity Capture-MS Homo sapiens
27 SOX2  
Affinity Capture-MS Homo sapiens
28 MMGT1 93380
Co-fractionation Homo sapiens
29 LAIR1  
Affinity Capture-MS Homo sapiens
30 TOMM70A 9868
Co-fractionation Homo sapiens
31 TCIRG1 10312
Co-fractionation Homo sapiens
32 HSPA4 3308
Reconstituted Complex Homo sapiens
33 PTRH2 51651
Co-fractionation Homo sapiens
34 KCNE3  
Affinity Capture-MS Homo sapiens
35 NDUFB9 4715
Co-fractionation Homo sapiens
36 NDUFS8 4728
Co-fractionation Homo sapiens
37 RDX 5962
Reconstituted Complex Homo sapiens
38 NDUFA2 4695
Co-fractionation Homo sapiens
39 RAB31 11031
Co-fractionation Homo sapiens
40 SYNJ2BP 55333
Co-fractionation Homo sapiens
41 Rdx 19684
Reconstituted Complex Mus musculus
42 RAB11B 9230
Co-fractionation Homo sapiens
43 SLC9A3R1 9368
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
44 CHCHD2  
Co-fractionation Homo sapiens
45 MRPL23 6150
Co-fractionation Homo sapiens
46 NFKB1 4790
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
47 CACNG2  
Affinity Capture-MS Homo sapiens
48 ATP6V1B1 525
Co-fractionation Homo sapiens
49 KTN1 3895
Cross-Linking-MS (XL-MS) Homo sapiens
50 NDUFA10 4705
Co-fractionation Homo sapiens
51 SSR1 6745
Co-fractionation Homo sapiens
52 PDZK1 5174
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
53 MCPH1  
Two-hybrid Homo sapiens
54 PIGH  
Affinity Capture-MS Homo sapiens
55 UBQLN1 29979
Two-hybrid Homo sapiens
56 MRPS26 64949
Co-fractionation Homo sapiens
57 PICALM 8301
Co-fractionation Homo sapiens
58 TUBB3 10381
Reconstituted Complex Homo sapiens
59 PDZD3  
Reconstituted Complex Homo sapiens
60 ATP6V1E1 529
Co-fractionation Homo sapiens
61 TOMM20 9804
Co-fractionation Homo sapiens
62 SLC30A5 64924
Co-fractionation Homo sapiens
63 S1PR4  
Affinity Capture-MS Homo sapiens
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