Experiment description of studies that identified INADL in sEVs

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 275 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

7 Experiment ID 274 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel resistant Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Flow cytometry Western blotting

8 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

9 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for INADL

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PARD3 56288 Proximity Label-MS Homo sapiens 2 ARHGAP17 55114 Affinity Capture-MS Homo sapiens 3 HOMER1   Two-hybrid Homo sapiens 4 HOMER3 9454 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 SNCA 6622 Affinity Capture-MS Homo sapiens 6 C2CD4B   Affinity Capture-MS Homo sapiens 7 Tmed2 56334 Affinity Capture-MS Mus musculus 8 Wwtr1   Affinity Capture-MS Mus musculus 9 GJB2   Affinity Capture-MS Homo sapiens 10 Lin7c 22343 Affinity Capture-MS Mus musculus 11 NECAB2   Two-hybrid Homo sapiens 12 CEP170P1   Affinity Capture-MS Homo sapiens 13 OCLN 100506658 Proximity Label-MS Homo sapiens 14 RNF166   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 ANLN 54443 Affinity Capture-MS Homo sapiens 16 CINP   Two-hybrid Homo sapiens 17 AMOTL2 51421 Affinity Capture-MS Homo sapiens 18 EPB41L4A 64097 Proximity Label-MS Homo sapiens 19 ANAPC15   Affinity Capture-MS Homo sapiens 20 FAM124A   Affinity Capture-MS Homo sapiens 21 RAB32 10981 Affinity Capture-MS Homo sapiens 22 GBA 2629 Affinity Capture-MS Homo sapiens 23 CAPZB 832 Co-fractionation Homo sapiens 24 METTL21B   Affinity Capture-MS Homo sapiens 25 PHYH 5264 Two-hybrid Homo sapiens 26 MPP5 64398 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 WWC1   Affinity Capture-MS Homo sapiens 28 GIPC2 54810 Two-hybrid Homo sapiens 29 LATS1   Proximity Label-MS Homo sapiens Proximity Label-MS Homo sapiens 30 PSMD14 10213 Affinity Capture-MS Homo sapiens 31 HSF2   Affinity Capture-MS Homo sapiens 32 LIN7A 8825 Affinity Capture-MS Homo sapiens 33 TJP3   Reconstituted Complex Homo sapiens 34 RNF146   Affinity Capture-Luminescence Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-Western Homo sapiens 35 RNASEH2A 10535 Affinity Capture-MS Homo sapiens 36 CDH1 999 Proximity Label-MS Homo sapiens 37 MLLT4 4301 Proximity Label-MS Homo sapiens 38 YAP1 10413 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 Tpx2   Affinity Capture-MS Mus musculus 40 C16orf72 29035 Affinity Capture-MS Homo sapiens 41 FLOT1 10211 Proximity Label-MS Homo sapiens 42 SAV1   Proximity Label-MS Homo sapiens 43 LIN7C 55327 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 Kif13b   Affinity Capture-MS Mus musculus 45 CIT 11113 Affinity Capture-MS Homo sapiens 46 DTX2 113878 Proximity Label-MS Homo sapiens 47 TP53RK 112858 Affinity Capture-MS Homo sapiens 48 PRC1 9055 Affinity Capture-MS Homo sapiens 49 AMOT 154796 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Proximity Label-MS Homo sapiens 50 CEP57L1   Two-hybrid Homo sapiens 51 Yap1   Affinity Capture-MS Mus musculus Affinity Capture-MS Mus musculus Reconstituted Complex Mus musculus 52 RHOB 388 Proximity Label-MS Homo sapiens 53 Nedd1   Affinity Capture-MS Mus musculus 54 CLDN1 9076 Reconstituted Complex Homo sapiens 55 Ufl1   Affinity Capture-MS Mus musculus 56 LATS2 26524 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 57 FHL2 2274 Affinity Capture-MS Homo sapiens 58 LIN7B   Affinity Capture-MS Homo sapiens 59 Plekhg5   Affinity Capture-MS Mus musculus 60 SLC33A1 9197 Affinity Capture-MS Homo sapiens 61 LURAP1   Affinity Capture-MS Homo sapiens 62 PCDHAC2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 BAI1   Protein-peptide Homo sapiens View the network

Pathways in which INADL is involved

No pathways found