Gene description for OLFM4
Gene name olfactomedin 4
Gene symbol OLFM4
Other names/aliases GC1
GW112
OLM4
OlfD
UNQ362
bA209J19.1
hGC-1
hOLfD
Species Homo sapiens
 Database cross references - OLFM4
ExoCarta ExoCarta_10562
Vesiclepedia VP_10562
Entrez Gene 10562
HGNC 17190
MIM 614061
UniProt Q6UX06  
 OLFM4 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Saliva 19199708    
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for OLFM4
Molecular Function
    structural molecule activity GO:0005198 IDA
    protein binding GO:0005515 IPI
    cadherin binding GO:0045296 IMP
Biological Process
    cell adhesion GO:0007155 IEA
    signal transduction GO:0007165 IBA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IMP
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    secretory granule GO:0030141 IDA
    protein-containing complex GO:0032991 IMP
    specific granule lumen GO:0035580 TAS
    specific granule GO:0042581 IDA
    azurophil granule GO:0042582 IDA
    intercellular bridge GO:0045171 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
    tertiary granule lumen GO:1904724 TAS
 Experiment description of studies that identified OLFM4 in sEVs
1
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
9
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for OLFM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TFR2 7036
Two-hybrid Homo sapiens
2 NID2 22795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TOR3A 64222
Affinity Capture-MS Homo sapiens
4 GJA8  
Affinity Capture-MS Homo sapiens
5 DDX11L8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RHBDD1 84236
Affinity Capture-MS Homo sapiens
7 AQP2 359
Two-hybrid Homo sapiens
8 FNDC9  
Two-hybrid Homo sapiens
9 CLEC7A  
Two-hybrid Homo sapiens
10 RBPMS 11030
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 P3H4 10609
Affinity Capture-MS Homo sapiens
12 ALDH16A1 126133
Affinity Capture-MS Homo sapiens
13 TMEM27 57393
Two-hybrid Homo sapiens
14 CLSTN3 9746
Two-hybrid Homo sapiens
15 IL11RA  
Two-hybrid Homo sapiens
16 EXOC8 149371
Affinity Capture-MS Homo sapiens
17 TAZ  
Affinity Capture-MS Homo sapiens
18 LDLRAD1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 KIR3DL3  
Two-hybrid Homo sapiens
20 TNFSF11  
Two-hybrid Homo sapiens
21 GNPTG 84572
Affinity Capture-MS Homo sapiens
22 TRUB2  
Affinity Capture-MS Homo sapiens
23 GNG8  
Affinity Capture-MS Homo sapiens
24 CLDN7 1366
Two-hybrid Homo sapiens
25 BSCL2  
Two-hybrid Homo sapiens
26 SYNE4  
Two-hybrid Homo sapiens
27 BMP7 655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TFB2M  
Affinity Capture-MS Homo sapiens
29 FUT8 2530
Affinity Capture-MS Homo sapiens
30 USP54 159195
Affinity Capture-MS Homo sapiens
31 TSPAN18 90139
Two-hybrid Homo sapiens
32 COL18A1 80781
Affinity Capture-MS Homo sapiens
33 CCDC155  
Two-hybrid Homo sapiens
34 AQP3  
Two-hybrid Homo sapiens
35 ZNF281  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 MTFR1L 56181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 KIR2DL3  
Two-hybrid Homo sapiens
38 CREB3L1  
Two-hybrid Homo sapiens
39 APP 351
Reconstituted Complex Homo sapiens
40 SUSD3 203328
Two-hybrid Homo sapiens
41 HSPA5 3309
Affinity Capture-MS Homo sapiens
42 LEUTX  
Two-hybrid Homo sapiens
43 EREG  
Two-hybrid Homo sapiens
44 FAM209A 200232
Two-hybrid Homo sapiens
45 FUT11 170384
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ERF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 KLK10 5655
Affinity Capture-MS Homo sapiens
48 EMILIN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 TMEM130  
Two-hybrid Homo sapiens
50 RDH13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 RPUSD3  
Affinity Capture-MS Homo sapiens
52 DNAJB9 4189
Affinity Capture-MS Homo sapiens
53 C17orf70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 WDR59 79726
Affinity Capture-MS Homo sapiens
55 EGFL8  
Affinity Capture-MS Homo sapiens
56 ETV3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PVRIG  
Two-hybrid Homo sapiens
58 DHFRL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 P3H3 10536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SLC22A23  
Two-hybrid Homo sapiens
61 TNFSF14  
Two-hybrid Homo sapiens
62 LHFPL2 10184
Two-hybrid Homo sapiens
63 ITM2C 81618
Two-hybrid Homo sapiens
64 SDF2L1 23753
Affinity Capture-MS Homo sapiens
65 PDF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 GNL3L 54552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 INTS6 26512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CLEC17A  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which OLFM4 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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