Experiment ID |
121 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|HSP60|HSP70
|
EV Cytosolic markers |
✔
CD81
|
EV Membrane markers |
✔
HSPA5
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
21276792
|
Organism |
Homo sapiens |
Experiment description |
Morphologic and proteomic characterization of exosomes released by cultured extravillous trophoblast cells. |
Authors |
Atay S, Gercel-Taylor C, Kesimer M, Taylor DD. |
Journal name |
ECR
|
Publication year |
2011 |
Sample |
Trophoblast cells |
Sample name |
Sw71 |
Isolation/purification methods |
Differential centrifugation Ultrafiltration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [Orbitrap] Western blotting |