Gene description for Cdc37
Gene name cell division cycle 37
Gene symbol Cdc37
Other names/aliases p50
p50Cdc37
Species Mus musculus
 Database cross references - Cdc37
ExoCarta ExoCarta_12539
Vesiclepedia VP_12539
Entrez Gene 12539
UniProt Q61081  
 Cdc37 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cdc37
Molecular Function
    transcription corepressor binding GO:0001222 ISO
    protein binding GO:0005515 IPI
    protein kinase regulator activity GO:0019887 IDA
    kinase binding GO:0019900 IPI
    kinase binding GO:0019900 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 ISO
    mitogen-activated protein kinase kinase kinase binding GO:0031435 ISO
    protein kinase B binding GO:0043422 ISO
    unfolded protein binding GO:0051082 IBA
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 ISO
    Hsp90 protein binding GO:0051879 IDA
    scaffold protein binding GO:0097110 IEA
    scaffold protein binding GO:0097110 ISO
Biological Process
    protein folding GO:0006457 IBA
    post-transcriptional regulation of gene expression GO:0010608 IDA
    regulation of protein kinase activity GO:0045859 IDA
    protein stabilization GO:0050821 IBA
    regulation of type II interferon-mediated signaling pathway GO:0060334 ISO
    regulation of type I interferon-mediated signaling pathway GO:0060338 ISO
    positive regulation of type 2 mitophagy GO:1905091 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    ruffle membrane GO:0032587 ISO
    protein-containing complex GO:0032991 ISO
    protein folding chaperone complex GO:0101031 ISO
    HSP90-CDC37 chaperone complex GO:1990565 IDA
    HSP90-CDC37 chaperone complex GO:1990565 ISO
 Experiment description of studies that identified Cdc37 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cdc37
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GDF15 9518
Affinity Capture-MS Homo sapiens
2 MRPL42  
Affinity Capture-MS Homo sapiens
3 WDR6 11180
Affinity Capture-MS Homo sapiens
4 FTSJ1 24140
Affinity Capture-MS Homo sapiens
5 DOCK5 80005
Affinity Capture-MS Homo sapiens
6 LCOR 84458
Affinity Capture-MS Homo sapiens
7 ARHGEF33  
Affinity Capture-MS Homo sapiens
8 Snx5 69178
Co-fractionation Mus musculus
9 EPB41L5 57669
Affinity Capture-MS Homo sapiens
10 IKBKG 8517
Affinity Capture-MS Homo sapiens
11 Cdk4  
Co-fractionation Mus musculus
12 Tomm34 67145
Co-fractionation Mus musculus
13 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
14 OBFC1 79991
Affinity Capture-MS Homo sapiens
15 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
16 Lrrk2  
Affinity Capture-Western Mus musculus
17 TUBA1A 7846
Affinity Capture-MS Homo sapiens
18 Mettl21e  
Affinity Capture-MS Mus musculus
19 Hsp90aa1 15519
Affinity Capture-Western Mus musculus
20 Tmem173  
Proximity Label-MS Mus musculus
21 Ryk  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
22 Ywhab 54401
Co-fractionation Mus musculus
23 Rpap3  
Co-fractionation Mus musculus
24 NFKBIL1  
Affinity Capture-MS Homo sapiens
25 LETMD1  
Affinity Capture-MS Homo sapiens
26 Syne2  
Co-fractionation Mus musculus
27 STX18 53407
Affinity Capture-MS Homo sapiens
28 IKBKB 3551
Affinity Capture-MS Homo sapiens
29 ARAF 369
Affinity Capture-MS Homo sapiens
30 Rpl4 67891
Co-fractionation Mus musculus
31 Fancd2  
Affinity Capture-MS Mus musculus
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