Gene description for SERPINA5
Gene name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
Gene symbol SERPINA5
Other names/aliases PAI-3
PAI3
PCI
PCI-B
PLANH3
PROCI
Species Homo sapiens
 Database cross references - SERPINA5
ExoCarta ExoCarta_5104
Vesiclepedia VP_5104
Entrez Gene 5104
HGNC 8723
MIM 601841
UniProt P05154  
 SERPINA5 identified in sEVs derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 21557262    
Urine 21557262    
Urine 22418980    
Urine 22418980    
Urine 25452312    
 Gene ontology annotations for SERPINA5
Molecular Function
    retinoic acid binding GO:0001972 IDA
    protease binding GO:0002020 IPI
    serine-type endopeptidase inhibitor activity GO:0004867 IDA
    protein binding GO:0005515 IPI
    glycosaminoglycan binding GO:0005539 TAS
    heparin binding GO:0008201 TAS
    phosphatidylcholine binding GO:0031210 IDA
    acrosin binding GO:0032190 IPI
Biological Process
    lipid transport GO:0006869 IEA
    spermatogenesis GO:0007283 ISS
    fusion of sperm to egg plasma membrane involved in single fertilization GO:0007342 NAS
    negative regulation of hydrolase activity GO:0051346 IMP
Subcellular Localization
    acrosomal membrane GO:0002080 IDA
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    external side of plasma membrane GO:0009897 IDA
    membrane GO:0016020 IDA
    platelet alpha granule GO:0031091 IDA
    platelet dense tubular network GO:0031094 IDA
    protein-containing complex GO:0032991 IDA
    protein C inhibitor-TMPRSS7 complex GO:0036024 IDA
    protein C inhibitor-TMPRSS11E complex GO:0036025 IDA
    protein C inhibitor-PLAT complex GO:0036026 IDA
    protein C inhibitor-PLAU complex GO:0036027 IDA
    protein C inhibitor-thrombin complex GO:0036028 IDA
    protein C inhibitor-KLK3 complex GO:0036029 IDA
    protein C inhibitor-plasma kallikrein complex GO:0036030 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
    protein C inhibitor-coagulation factor V complex GO:0097181 IDA
    protein C inhibitor-coagulation factor Xa complex GO:0097182 IDA
    protein C inhibitor-coagulation factor XI complex GO:0097183 IDA
 Experiment description of studies that identified SERPINA5 in sEVs
1
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 114
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors "Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 113
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors "Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 238
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors "Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R."
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SERPINA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLAT 5327
Reconstituted Complex Homo sapiens
2 CTAGE5 4253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 FURIN 5045
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CANX 821
Affinity Capture-MS Homo sapiens
5 WWOX 51741
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HECTD3 79654
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PROC 5624
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
8 ZZEF1 23140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RCHY1  
Two-hybrid Homo sapiens
10 ACR  
Reconstituted Complex Homo sapiens
11 PIAS4  
Two-hybrid Homo sapiens
12 HRSP12 10247
Two-hybrid Homo sapiens
13 KLK2  
Affinity Capture-Western Homo sapiens
14 F11 2160
Reconstituted Complex Homo sapiens
15 EPSTI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PLAU 5328
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
17 KLK3  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
18 FBXO2 26232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 COPS6 10980
Two-hybrid Homo sapiens
20 KLKB1 3818
Reconstituted Complex Homo sapiens
21 DHH  
Affinity Capture-MS Homo sapiens
22 MIA3 375056
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HGFAC 3083
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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