Gene description for Copb2
Gene name coatomer protein complex, subunit beta 2 (beta prime)
Gene symbol Copb2
Other names/aliases AI256832
Species Mus musculus
 Database cross references - Copb2
ExoCarta ExoCarta_50797
Vesiclepedia VP_50797
Entrez Gene 50797
UniProt O55029  
 Copb2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Copb2
Molecular Function
    protein kinase C binding GO:0005080 ISO
    structural molecule activity GO:0005198 IEA
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISO
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISS
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    intra-Golgi vesicle-mediated transport GO:0006891 IEA
    intra-Golgi vesicle-mediated transport GO:0006891 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    COPI vesicle coat GO:0030126 IBA
    COPI vesicle coat GO:0030126 ISO
 Experiment description of studies that identified Copb2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Copb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Sacm1l 83493
Co-fractionation Mus musculus
4 Rpl27 19942
Co-fractionation Mus musculus
5 Foxp3  
Affinity Capture-MS Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Ndn  
Two-hybrid Mus musculus
8 Sec23a  
Co-fractionation Mus musculus
9 Unk  
Affinity Capture-RNA Mus musculus
10 Rpl23 65019
Co-fractionation Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Copb1 70349
Co-fractionation Mus musculus
13 Uba5  
Co-fractionation Mus musculus
14 Nudcd1  
Co-fractionation Mus musculus
View the network image/svg+xml



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