Gene description for CHSY3
Gene name chondroitin sulfate synthase 3
Gene symbol CHSY3
Other names/aliases CHSY2
CSS3
Species Homo sapiens
 Database cross references - CHSY3
ExoCarta ExoCarta_337876
Vesiclepedia VP_337876
Entrez Gene 337876
HGNC 24293
MIM 609963
UniProt Q70JA7  
 CHSY3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CHSY3
Molecular Function
    metal ion binding GO:0046872 IEA
    glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity GO:0047238 IBA
    glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity GO:0047238 TAS
    N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity GO:0050510 TAS
Biological Process
    chondroitin sulfate biosynthetic process GO:0030206 IBA
    chondroitin sulfate biosynthetic process GO:0030206 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified CHSY3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CHSY3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCL3  
Affinity Capture-MS Homo sapiens
2 LRP5 4041
Affinity Capture-MS Homo sapiens
3 CHPF 79586
Affinity Capture-MS Homo sapiens
4 CMTM6 54918
Affinity Capture-MS Homo sapiens
5 HSPA5 3309
Affinity Capture-MS Homo sapiens
6 CEACAM8 1088
Affinity Capture-MS Homo sapiens
7 P3H4 10609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SIAE 54414
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 LCN6  
Affinity Capture-MS Homo sapiens
10 STK17B 9262
Affinity Capture-MS Homo sapiens
11 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PDGFRA 5156
Affinity Capture-MS Homo sapiens
13 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TLR5  
Affinity Capture-MS Homo sapiens
15 RMND1 55005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MGAT4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GGT7 2686
Affinity Capture-MS Homo sapiens
18 OS9 10956
Affinity Capture-MS Homo sapiens
19 CCPG1 9236
Affinity Capture-MS Homo sapiens
20 MYCBPAP 84073
Affinity Capture-MS Homo sapiens
21 CHPF2 54480
Affinity Capture-MS Homo sapiens
22 CLEC12B  
Affinity Capture-MS Homo sapiens
23 ADAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GDPD5 81544
Affinity Capture-MS Homo sapiens
25 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 C1orf54  
Affinity Capture-MS Homo sapiens
27 FOXN3 1112
Affinity Capture-MS Homo sapiens
28 NMU  
Affinity Capture-MS Homo sapiens
29 RCN2 5955
Affinity Capture-MS Homo sapiens
30 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CHSY1 22856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 LRFN4  
Affinity Capture-MS Homo sapiens
33 MAN1A1 4121
Affinity Capture-MS Homo sapiens
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