Gene description for IMPAD1
Gene name inositol monophosphatase domain containing 1
Gene symbol IMPAD1
Other names/aliases GPAPP
IMP 3
IMP-3
IMPA3
Species Homo sapiens
 Database cross references - IMPAD1
ExoCarta ExoCarta_54928
Vesiclepedia VP_54928
Entrez Gene 54928
HGNC 26019
MIM 614010
UniProt Q9NX62  
 IMPAD1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for IMPAD1
Molecular Function
    3'-nucleotidase activity GO:0008254 IBA
    3'(2'),5'-bisphosphate nucleotidase activity GO:0008441 IEA
    metal ion binding GO:0046872 IEA
    3',5'-nucleotide bisphosphate phosphatase activity GO:0097657 ISS
Biological Process
    skeletal system development GO:0001501 IBA
    endochondral ossification GO:0001958 IEA
    chondrocyte development GO:0002063 IEA
    post-embryonic development GO:0009791 IEA
    chondroitin sulfate metabolic process GO:0030204 IEA
    embryonic digit morphogenesis GO:0042733 IEA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi lumen GO:0005796 TAS
    cytosol GO:0005829 IDA
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 HDA
    nuclear body GO:0016604 IDA
    trans-Golgi network membrane GO:0032588 ISS
 Experiment description of studies that identified IMPAD1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IMPAD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UQCC3  
Co-fractionation Homo sapiens
2 RAB39B 116442
Affinity Capture-MS Homo sapiens
3 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
4 PARP1 142
Cross-Linking-MS (XL-MS) Homo sapiens
5 ABCB8 11194
Co-fractionation Homo sapiens
6 TNFSF13B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GOLPH3 64083
Affinity Capture-MS Homo sapiens
8 SDHA 6389
Co-fractionation Homo sapiens
9 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 OCRL 4952
Affinity Capture-MS Homo sapiens
11 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CD1B 910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EFNA5  
Affinity Capture-MS Homo sapiens
16 PLCB4 5332
Cross-Linking-MS (XL-MS) Homo sapiens
17 CYB5R3 1727
Co-fractionation Homo sapiens
18 C1orf198 84886
Affinity Capture-MS Homo sapiens
19 ATP1B3 483
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PCDHB11  
Affinity Capture-MS Homo sapiens
21 KIAA1429 25962
Affinity Capture-MS Homo sapiens
22 CLEC12B  
Affinity Capture-MS Homo sapiens
23 ST3GAL4 6484
Affinity Capture-MS Homo sapiens
24 PLTP 5360
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
26 SOAT1 6646
Affinity Capture-MS Homo sapiens
27 TOMM6  
Co-fractionation Homo sapiens
28 PCDHGC5  
Affinity Capture-MS Homo sapiens
29 LCN9  
Affinity Capture-MS Homo sapiens
30 TMPRSS13  
Affinity Capture-MS Homo sapiens
31 SCGB2A2  
Affinity Capture-MS Homo sapiens
32 CRP  
Affinity Capture-MS Homo sapiens
33 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PTPRN  
Affinity Capture-MS Homo sapiens
35 SERBP1 26135
Affinity Capture-MS Homo sapiens
36 PON2 5445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 DDRGK1 65992
Co-fractionation Homo sapiens
38 MPPE1 65258
Affinity Capture-MS Homo sapiens
39 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
40 PCDHGA7 56108
Affinity Capture-MS Homo sapiens
41 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SEC61B 10952
Proximity Label-MS Homo sapiens
43 FAM20C 56975
Affinity Capture-MS Homo sapiens
44 UST  
Affinity Capture-MS Homo sapiens
45 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
46 MAN1A1 4121
Affinity Capture-MS Homo sapiens
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