Gene description for PIK3C3
Gene name phosphatidylinositol 3-kinase, catalytic subunit type 3
Gene symbol PIK3C3
Other names/aliases VPS34
Vps34
hVps34
Species Homo sapiens
 Database cross references - PIK3C3
ExoCarta ExoCarta_5289
Vesiclepedia VP_5289
Entrez Gene 5289
HGNC 8974
MIM 602609
UniProt Q8NEB9  
 PIK3C3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PIK3C3
Molecular Function
    protein kinase activity GO:0004672 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IBA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IDA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IMP
    1-phosphatidylinositol-3-kinase activity GO:0016303 TAS
    phosphatidylinositol kinase activity GO:0052742 IDA
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IDA
    pexophagy GO:0000425 IBA
    protein targeting to lysosome GO:0006622 NAS
    endocytosis GO:0006897 IBA
    autophagy GO:0006914 IMP
    regulation of autophagy GO:0010506 IDA
    macroautophagy GO:0016236 ISS
    macroautophagy GO:0016236 TAS
    regulation of macroautophagy GO:0016241 IDA
    regulation of cytokinesis GO:0032465 IMP
    protein localization to phagophore assembly site GO:0034497 IEA
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IBA
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IDA
    cellular response to glucose starvation GO:0042149 ISS
    positive regulation by host of viral genome replication GO:0044829 IDA
    early endosome to late endosome transport GO:0045022 IDA
    early endosome to late endosome transport GO:0045022 IMP
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IDA
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    synaptic vesicle endocytosis GO:0048488 IEA
    cell division GO:0051301 IEA
    autophagosome maturation GO:0097352 IDA
    positive regulation of protein lipidation GO:1903061 IMP
Subcellular Localization
    phagophore assembly site GO:0000407 IBA
    cytoplasm GO:0005737 IBA
    endosome GO:0005768 IBA
    late endosome GO:0005770 IDA
    autophagosome GO:0005776 IEA
    peroxisome GO:0005777 IBA
    cytosol GO:0005829 TAS
    axoneme GO:0005930 ISS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    midbody GO:0030496 IDA
    phagocytic vesicle membrane GO:0030670 TAS
    phosphatidylinositol 3-kinase complex, class III, type I GO:0034271 IBA
    phosphatidylinositol 3-kinase complex, class III, type II GO:0034272 IBA
    phosphatidylinositol 3-kinase complex, class III GO:0035032 IPI
    phosphatidylinositol 3-kinase complex, class III GO:0035032 ISS
    autolysosome GO:0044754 IDA
    presynaptic endosome GO:0098830 IEA
    postsynaptic endosome GO:0098845 IEA
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified PIK3C3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PIK3C3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 ZRANB1 54764
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
3 MAP1LC3C  
Affinity Capture-MS Homo sapiens
4 KIF1B 23095
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 RIT1 6016
Negative Genetic Homo sapiens
7 OCIAD1 54940
Affinity Capture-MS Homo sapiens
8 LAMTOR1 55004
Proximity Label-MS Homo sapiens
9 PSMD4 5710
Affinity Capture-Western Homo sapiens
10 USP11 8237
Affinity Capture-MS Homo sapiens
11 TP53 7157
Affinity Capture-MS Homo sapiens
12 EXOC8 149371
Affinity Capture-Western Homo sapiens
13 AMBRA1  
Affinity Capture-Western Homo sapiens
14 RAB5A 5868
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
15 JOSD1  
Affinity Capture-Western Homo sapiens
16 EXOC2 55770
Affinity Capture-Western Homo sapiens
17 KIAA0226L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PRMT5 10419
Affinity Capture-MS Homo sapiens
19 LGALS7B 653499
Affinity Capture-MS Homo sapiens
20 DBN1 1627
Affinity Capture-MS Homo sapiens
21 DACT1 51339
Affinity Capture-Western Homo sapiens
22 SPSB4  
Affinity Capture-MS Homo sapiens
23 Vmp1  
Affinity Capture-Western Rattus norvegicus
24 ATG101  
Affinity Capture-Western Homo sapiens
25 LAMP2 3920
Proximity Label-MS Homo sapiens
26 PIK3R4 30849
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
27 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
28 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
29 ATG14  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 PDPK1 5170
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 LGALS7 3963
Affinity Capture-MS Homo sapiens
32 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
33 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
34 RAB4A 5867
Proximity Label-MS Homo sapiens
35 UBE3C 9690
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
36 MYH1  
Affinity Capture-MS Homo sapiens
37 UVRAG  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
38 EGFR 1956
Negative Genetic Homo sapiens
39 GYPA  
Affinity Capture-MS Homo sapiens
40 SKAP1 8631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PGK1 5230
Affinity Capture-Western Homo sapiens
42 BECN1 8678
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
44 MYH9 4627
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 HSPA5 3309
Affinity Capture-MS Homo sapiens
47 JADE2 23338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 KRIT1 889
Affinity Capture-Western Homo sapiens
49 RAB9A 9367
Proximity Label-MS Homo sapiens
50 NRBF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 USP19 10869
Affinity Capture-Western Homo sapiens
52 HSPA1A 3303
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
53 JOSD2  
Affinity Capture-Western Homo sapiens
54 CLTB 1212
Proximity Label-MS Homo sapiens
55 YWHAQ 10971
Reconstituted Complex Homo sapiens
56 CLPTM1L 81037
Affinity Capture-MS Homo sapiens
57 CUL7 9820
Affinity Capture-MS Homo sapiens
58 RNF2  
Affinity Capture-Western Homo sapiens
59 GNB2L1 10399
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 TRIM28 10155
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
61 HECTD1 25831
Affinity Capture-MS Homo sapiens
62 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 OTUD7B 56957
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
64 CAPZB 832
Affinity Capture-MS Homo sapiens
65 USP28 57646
Affinity Capture-Western Homo sapiens
66 CYLD  
Affinity Capture-Western Homo sapiens
67 KIAA0226  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PIK3C3 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Antigen Presentation: Folding, assembly and peptide loading of class I MHC TAS Reactome
Autophagy TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Early SARS-CoV-2 Infection Events IEA Reactome
IGF1R signaling cascade TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Insulin receptor signalling cascade TAS Reactome
IRS-mediated signalling TAS Reactome
IRS-related events triggered by IGF1R TAS Reactome
Macroautophagy TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Phospholipid metabolism TAS Reactome
PI Metabolism TAS Reactome
PI3K Cascade TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate NADPH Oxidases TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV Infections IEA Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2 Infection IEA Reactome
SARS-CoV-2-host interactions TAS Reactome
Signal Transduction TAS Reactome
Signaling by Insulin receptor TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) TAS Reactome
Synthesis of PIPs at the early endosome membrane TAS Reactome
Synthesis of PIPs at the Golgi membrane TAS Reactome
Synthesis of PIPs at the late endosome membrane TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
Translation of Replicase and Assembly of the Replication Transcription Complex TAS Reactome
Translation of Replicase and Assembly of the Replication Transcription Complex IEA Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome





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