Gene description for NT5C3A
Gene name 5'-nucleotidase, cytosolic IIIA
Gene symbol NT5C3A
Other names/aliases NT5C3
P5'N-1
P5N-1
PN-I
POMP
PSN1
UMPH
UMPH1
cN-III
hUMP1
p36
Species Homo sapiens
 Database cross references - NT5C3A
ExoCarta ExoCarta_51251
Vesiclepedia VP_51251
Entrez Gene 51251
HGNC 17820
MIM 606224
UniProt Q9H0P0  
 NT5C3A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NT5C3A
Molecular Function
    nucleotide binding GO:0000166 IEA
    tRNA 2'-phosphotransferase activity GO:0000215 NAS
    tRNA 2'-phosphotransferase activity GO:0000215 TAS
    magnesium ion binding GO:0000287 NAS
    protein binding GO:0005515 IPI
    5'-nucleotidase activity GO:0008253 IBA
    5'-nucleotidase activity GO:0008253 IDA
    5'-nucleotidase activity GO:0008253 NAS
    XMP 5'-nucleosidase activity GO:0106411 IEA
Biological Process
    pyrimidine nucleoside metabolic process GO:0006213 NAS
    nucleotide metabolic process GO:0009117 IEA
    defense response to virus GO:0051607 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    nuclear body GO:0016604 IDA
 Experiment description of studies that identified NT5C3A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NT5C3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CALML3 810
Affinity Capture-MS Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 CHRNA4  
Affinity Capture-MS Homo sapiens
5 cher  
Two-hybrid Drosophila melanogaster
6 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
7 AGPAT1 10554
Affinity Capture-MS Homo sapiens
8 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
9 TP53 7157
Synthetic Growth Defect Homo sapiens
10 MZT1  
Affinity Capture-MS Homo sapiens
11 SPAG6  
Affinity Capture-MS Homo sapiens
12 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 BZW2 28969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RSPH14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 UBQLN2 29978
Two-hybrid Homo sapiens
16 GPC3 2719
Affinity Capture-MS Homo sapiens
17 UBQLN1 29979
Two-hybrid Homo sapiens
18 MTAP 4507
Affinity Capture-MS Homo sapiens
19 GNAQ 2776
Two-hybrid Homo sapiens
20 CDH23 64072
Affinity Capture-MS Homo sapiens
21 DPEP1 1800
Affinity Capture-MS Homo sapiens
22 CALM1 801
Affinity Capture-MS Homo sapiens
23 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GHITM 27069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SOX2  
Affinity Capture-MS Homo sapiens
26 SLC6A1  
Affinity Capture-MS Homo sapiens
27 OCIAD1 54940
Proximity Label-MS Homo sapiens
28 CDC42 998
Affinity Capture-MS Homo sapiens
29 DPPA4  
Affinity Capture-MS Homo sapiens
30 CPVL 54504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RPA3 6119
Proximity Label-MS Homo sapiens
33 RNF115  
Affinity Capture-MS Homo sapiens
34 Mis12  
Affinity Capture-MS Mus musculus
35 Sesn2  
Affinity Capture-MS Mus musculus
36 GOT1 2805
Affinity Capture-MS Homo sapiens
37 ORAI1 84876
Affinity Capture-MS Homo sapiens
38 SARAF  
Affinity Capture-MS Homo sapiens
39 SSUH2  
Affinity Capture-MS Homo sapiens
40 NDUFAB1 4706
Two-hybrid Homo sapiens
41 ABCA2 20
Affinity Capture-MS Homo sapiens
42 C9orf72  
Affinity Capture-MS Homo sapiens
43 OR2A4 79541
Affinity Capture-MS Homo sapiens
44 ADAM18  
Affinity Capture-MS Homo sapiens
45 RAB11B 9230
Affinity Capture-MS Homo sapiens
46 VANGL1 81839
Two-hybrid Homo sapiens
47 CCR1  
Affinity Capture-MS Homo sapiens
48 Cenpe  
Affinity Capture-MS Mus musculus
49 STX17 55014
Affinity Capture-MS Homo sapiens
50 AKAP1 8165
Proximity Label-MS Homo sapiens
51 FAM86C1  
Affinity Capture-MS Homo sapiens
52 KCNA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 PHF11 51131
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NT5C3A is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of nucleotides TAS Reactome
Nucleotide catabolism TAS Reactome
Pyrimidine catabolism TAS Reactome





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