Gene ontology annotations for HLA-DRA
Experiment description of studies that identified HLA-DRA in exosomes
1
Experiment ID
110
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
CD81|CD9|HLA-DR|EpCAM
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21601258
Organism
Homo sapiens
Experiment description
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name
GO
Publication year
2011
Sample
Ascites
Sample name
Ascites - Liver cirrhosis
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.04-1.10 g/mL
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Immunoelctron microscopy
2
Experiment ID
37
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD86
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
10878338
Organism
Homo sapiens
Experiment description
Direct immunosuppressive effects of EBV-encoded latent membrane protein 1.
Authors
Dukers DF, Meij P, Vervoort MB, Vos W, Scheper RJ, Meijer CJ, Bloemena E, Middeldorp JM
Journal name
JIMMU
Publication year
2000
Sample
B cell lymphoblastoid cells
Sample name
JY B95-8
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
3
Experiment ID
11
ISEV standards
✘
EV Biophysical techniques
✔
HSC70|HSP90
EV Cytosolic markers
✔
MHCII|CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [MALDI TOF]
PubMed ID
12519789
Organism
Homo sapiens
Experiment description
Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors
Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name
JBC
Publication year
2003
Sample
B cells
Sample name
RN (HLA-DR15+)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting Thin layer chromatography HPLC
4
Experiment ID
47
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|CD86
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
17641064
Organism
Homo sapiens
Experiment description
Exosomes with immune modulatory features are present in human breast milk.
Authors
Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name
JIMMU
Publication year
2007
Sample
B cells
Sample name
B cells
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS
5
Experiment ID
79
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
6
Experiment ID
80
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
7
Experiment ID
81
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
8
Experiment ID
42
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
15908444
Organism
Homo sapiens
Experiment description
Exosomal-like vesicles are present in human blood plasma.
Authors
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name
INTIMM
Publication year
2005
Sample
Bone marrow cells
Sample name
PBMC
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
9
Experiment ID
46
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|MHCII|CD81|CD86
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy Western blotting FACS
PubMed ID
17641064
Organism
Homo sapiens
Experiment description
Exosomes with immune modulatory features are present in human breast milk.
Authors
Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name
JIMMU
Publication year
2007
Sample
Breast milk
Sample name
Breast milk - Colostrum
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.10-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy FACS
10
Experiment ID
48
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|CD86|MHCII
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy Western blotting FACS
PubMed ID
17641064
Organism
Homo sapiens
Experiment description
Exosomes with immune modulatory features are present in human breast milk.
Authors
Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name
JIMMU
Publication year
2007
Sample
Breast milk
Sample name
Breast milk - Mature milk
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.10-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy FACS
11
Experiment ID
46
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|MHCII|CD81|CD86
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17641064
Organism
Homo sapiens
Experiment description
Exosomes with immune modulatory features are present in human breast milk.
Authors
Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name
JIMMU
Publication year
2007
Sample
Breast milk
Sample name
Breast milk - Colostrum
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.10-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy FACS
12
Experiment ID
49
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD86
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS Immunoelectron Microscopy
PubMed ID
14582906
Organism
Homo sapiens
Experiment description
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors
Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name
ERJ
Publication year
2003
Sample
Bronchoalveolar lavage fluid
Sample name
Bronchoalveolar lavage fluid
Isolation/purification methods
Differential centrifugation Immunobeads (EpCAM)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy
13
Experiment ID
50
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD87
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS Immunoelectron Microscopy
PubMed ID
14582906
Organism
Homo sapiens
Experiment description
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors
Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name
ERJ
Publication year
2003
Sample
Dendritic cells
Sample name
Monocyte-derived dendritic cells
Isolation/purification methods
Differential centrifugation Immunobeads (EpCAM)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy
14
Experiment ID
235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
15
Experiment ID
4
ISEV standards
✔
IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|MHCI|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
11487543
Organism
Homo sapiens
Experiment description
Intestinal epithelial cells secrete exosome-like vesicles.
Authors
van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M
Journal name
Publication year
2001
Sample
Intestinal epithelial cells
Sample name
HT29-19A T84-DRB1*0401/CIITA
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
16
Experiment ID
56
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
MHCI|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy Western blotting
PubMed ID
12147373
Organism
Homo sapiens
Experiment description
Malignant effusions and immunogenic tumour-derived exosomes.
Authors
Andre F, Schartz NE, Movassagh M, Flament C, Pautier P, Morice P, Pomel C, Lhomme C, Escudier B, Le Chevalier T, Tursz T, Amigorena S, Raposo G, Angevin E, Zitvogel L
Journal name
LANCET
Publication year
2002
Sample
Malignant ascites
Sample name
Malignant ascites
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.14-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting
17
Experiment ID
108
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
CD81|CD9|EpCAM
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21601258
Organism
Homo sapiens
Experiment description
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name
GO
Publication year
2011
Sample
Malignant ascites
Sample name
Malignant ascites - Ovarian cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (EPCAM, CD24)
Flotation density
1.04-1.15 g/mL
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Immunoelctron microscopy
18
Experiment ID
51
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
14975938
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors
Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name
AJRCMB
Publication year
2004
Sample
Malignant pleural effusions
Sample name
Pleural Fluid - Breast cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
19
Experiment ID
52
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
14975938
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors
Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name
AJRCMB
Publication year
2004
Sample
Malignant pleural effusions
Sample name
Pleural Fluid - Lung cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
20
Experiment ID
53
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
14975938
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors
Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name
AJRCMB
Publication year
2004
Sample
Malignant pleural effusions
Sample name
Pleural Fluid - Mesothelioma
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
21
Experiment ID
54
ISEV standards
✘
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
14975938
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors
Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name
AJRCMB
Publication year
2004
Sample
Malignant pleural effusions
Sample name
Pleural Fluid - Ovarian adenocarcioma
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
22
Experiment ID
40
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9|MHCII|TFRC|LAMP2|ITGA2B
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS Western blotting
PubMed ID
15908444
Organism
Homo sapiens
Experiment description
Exosomal-like vesicles are present in human blood plasma.
Authors
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name
INTIMM
Publication year
2005
Sample
Plasma
Sample name
Plasma - Normal
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.15-1.27 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting FACS Immunoelectron Microscopy
23
Experiment ID
231
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
24
Experiment ID
232
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
25
Experiment ID
233
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
26
Experiment ID
109
ISEV standards
✘
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
CD9|MHCII|HLA-DR|CD24|EpCAM
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21601258
Organism
Homo sapiens
Experiment description
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name
GO
Publication year
2011
Sample
Pleural effusions
Sample name
Pleural effusions - Breast cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (EPCAM)
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting
27
Experiment ID
66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19199708
Organism
Homo sapiens
Experiment description
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name
JPR
Publication year
2009
Sample
Saliva
Sample name
Saliva
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy
28
Experiment ID
217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
29
Experiment ID
63
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
Protein-protein interactions for HLA-DRA
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
MBP
Co-crystal Structure
Homo sapiens
2
HLA-DRB1
Affinity Capture-MS
Homo sapiens
3
CD63
967
Affinity Capture-Western
Homo sapiens
4
HLA-DRB3
3125
Affinity Capture-MS
Homo sapiens
5
HLA-DMA
Invivo
Homo sapiens
Invitro
Homo sapiens
6
HLA-DMB
3109
Invivo
Homo sapiens
Invivo
Homo sapiens
Invivo
Homo sapiens
Invivo
Homo sapiens
Invitro
Homo sapiens
Invitro
Homo sapiens
Invitro
Homo sapiens
Invitro
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
7
CD82
3732
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
8
HLA-DRB5
3127
Invitro
Homo sapiens
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which HLA-DRA is involved