Gene description for GSK3B
Gene name glycogen synthase kinase 3 beta
Gene symbol GSK3B
Other names/aliases -
Species Homo sapiens
 Database cross references - GSK3B
ExoCarta ExoCarta_2932
Vesiclepedia VP_2932
Entrez Gene 2932
HGNC 4617
MIM 605004
UniProt P49841  
 GSK3B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for GSK3B
Molecular Function
    protease binding GO:0002020 IPI
    p53 binding GO:0002039 IDA
    protein kinase activity GO:0004672 IMP
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 IMP
    protein serine/threonine kinase activity GO:0004674 ISS
    protein serine/threonine kinase activity GO:0004674 NAS
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    beta-catenin binding GO:0008013 IBA
    beta-catenin binding GO:0008013 IPI
    kinase activity GO:0016301 IDA
    protein kinase binding GO:0019901 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    protein kinase A catalytic subunit binding GO:0034236 IPI
    dynactin binding GO:0034452 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    tau protein binding GO:0048156 NAS
    tau-protein kinase activity GO:0050321 IBA
    tau-protein kinase activity GO:0050321 IDA
    tau-protein kinase activity GO:0050321 NAS
    NF-kappaB binding GO:0051059 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    scaffold protein binding GO:0097110 IPI
    protein serine kinase activity GO:0106310 IGI
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    epithelial to mesenchymal transition GO:0001837 IMP
    positive regulation of cell-matrix adhesion GO:0001954 IMP
    glycogen metabolic process GO:0005977 IDA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 IMP
    protein phosphorylation GO:0006468 TAS
    ER overload response GO:0006983 IDA
    mitochondrion organization GO:0007005 IMP
    G protein-coupled dopamine receptor signaling pathway GO:0007212 NAS
    circadian rhythm GO:0007623 ISS
    insulin receptor signaling pathway GO:0008286 IBA
    positive regulation of autophagy GO:0010508 IBA
    positive regulation of autophagy GO:0010508 ISS
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISS
    negative regulation of gene expression GO:0010629 IMP
    negative regulation of epithelial to mesenchymal transition GO:0010719 IDA
    regulation of neuron projection development GO:0010975 IBA
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-threonine phosphorylation GO:0018107 IDA
    viral protein processing GO:0019082 TAS
    hippocampus development GO:0021766 IMP
    establishment of cell polarity GO:0030010 ISS
    establishment of cell polarity GO:0030010 TAS
    maintenance of cell polarity GO:0030011 ISS
    maintenance of cell polarity GO:0030011 TAS
    cell differentiation GO:0030154 IBA
    negative regulation of cell migration GO:0030336 IDA
    regulation of axon extension GO:0030516 ISS
    neuron projection development GO:0031175 IDA
    negative regulation of protein-containing complex assembly GO:0031333 IMP
    positive regulation of protein-containing complex assembly GO:0031334 IDA
    positive regulation of protein ubiquitination GO:0031398 IDA
    negative regulation of TOR signaling GO:0032007 IBA
    positive regulation of protein binding GO:0032092 ISS
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IBA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IC
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IDA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    regulation of microtubule-based process GO:0032886 IMP
    intracellular signal transduction GO:0035556 IDA
    intracellular signal transduction GO:0035556 TAS
    cellular response to interleukin-3 GO:0036016 ISS
    regulation of circadian rhythm GO:0042752 ISS
    negative regulation of apoptotic process GO:0043066 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 NAS
    positive regulation of neuron apoptotic process GO:0043525 IBA
    positive regulation of GTPase activity GO:0043547 IMP
    positive regulation of cell differentiation GO:0045597 IMP
    negative regulation of osteoblast differentiation GO:0045668 IMP
    negative regulation of glycogen biosynthetic process GO:0045719 TAS
    positive regulation of cilium assembly GO:0045724 ISS
    positive regulation of protein catabolic process GO:0045732 IC
    protein autophosphorylation GO:0046777 IDA
    regulation of protein export from nucleus GO:0046825 IDA
    regulation of protein export from nucleus GO:0046825 ISS
    positive regulation of protein export from nucleus GO:0046827 IDA
    regulation of dendrite morphogenesis GO:0048814 ISS
    regulation of axonogenesis GO:0050770 ISS
    canonical Wnt signaling pathway GO:0060070 IDA
    canonical Wnt signaling pathway GO:0060070 ISS
    excitatory postsynaptic potential GO:0060079 NAS
    regulation of microtubule cytoskeleton organization GO:0070507 IBA
    regulation of microtubule cytoskeleton organization GO:0070507 ISS
    negative regulation of calcineurin-NFAT signaling cascade GO:0070885 IMP
    superior temporal gyrus development GO:0071109 IMP
    cellular response to retinoic acid GO:0071300 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IBA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IC
    negative regulation of canonical Wnt signaling pathway GO:0090090 IGI
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISS
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    extrinsic apoptotic signaling pathway GO:0097191 ISS
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 ISS
    presynaptic modulation of chemical synaptic transmission GO:0099171 EXP
    presynaptic modulation of chemical synaptic transmission GO:0099171 IDA
    presynaptic modulation of chemical synaptic transmission GO:0099171 IMP
    neuron projection organization GO:0106027 ISS
    regulation of microtubule anchoring at centrosome GO:0150101 IMP
    regulation of cellular response to heat GO:1900034 TAS
    negative regulation of protein localization to nucleus GO:1900181 ISS
    regulation of long-term synaptic potentiation GO:1900271 ISS
    positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901030 ISS
    negative regulation of protein acetylation GO:1901984 ISS
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IMP
    positive regulation of protein localization to cilium GO:1903566 ISS
    negative regulation of TORC2 signaling GO:1903940 IDA
    negative regulation of dopaminergic neuron differentiation GO:1904339 TAS
    cellular response to amyloid-beta GO:1904646 ISS
    positive regulation of protein localization to centrosome GO:1904781 IMP
    beta-catenin destruction complex disassembly GO:1904886 TAS
    negative regulation of type B pancreatic cell development GO:2000077 TAS
    negative regulation of glycogen (starch) synthase activity GO:2000466 TAS
    negative regulation of mesenchymal stem cell differentiation GO:2000740 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IEA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    axon GO:0030424 IBA
    axon GO:0030424 ISS
    dendrite GO:0030425 ISS
    beta-catenin destruction complex GO:0030877 IBA
    beta-catenin destruction complex GO:0030877 IDA
    beta-catenin destruction complex GO:0030877 NAS
    beta-catenin destruction complex GO:0030877 TAS
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IBA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    Wnt signalosome GO:1990909 TAS
 Experiment description of studies that identified GSK3B in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 226
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
9
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 237
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
13
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GSK3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC4  
Biochemical Activity Homo sapiens
2 NOTCH3 4854
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 PPP1CB 5500
Affinity Capture-MS Homo sapiens
4 PKM 5315
Co-fractionation Homo sapiens
5 NFATC1 4772
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
6 SYNE4  
Two-hybrid Homo sapiens
7 MAP4 4134
Two-hybrid Homo sapiens
8 UBE3A 7337
Affinity Capture-Western Homo sapiens
9 AKT3  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
10 PRKDC 5591
Biochemical Activity Homo sapiens
11 ADIRF 10974
Two-hybrid Homo sapiens
12 LPCAT1 79888
Biochemical Activity Homo sapiens
13 SNRNP70 6625
Affinity Capture-MS Homo sapiens
14 MTF2  
Two-hybrid Homo sapiens
15 PPP2R5D 5528
Affinity Capture-Western Homo sapiens
16 DEFA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SF3B1 23451
Affinity Capture-MS Homo sapiens
18 FAM193B 54540
Two-hybrid Homo sapiens
19 TRIOBP 11078
Affinity Capture-Western Homo sapiens
20 NCOA3  
Biochemical Activity Homo sapiens
21 RPF2 84154
Affinity Capture-MS Homo sapiens
22 DYNLL1 8655
Affinity Capture-MS Homo sapiens
23 HERC2 8924
Affinity Capture-MS Homo sapiens
24 ZNF598 90850
Affinity Capture-MS Homo sapiens
25 NSFL1C 55968
Two-hybrid Homo sapiens
26 BTRC 8945
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 VWA8 23078
Proximity Label-MS Homo sapiens
28 EEF1A1 1915
Two-hybrid Homo sapiens
29 Ctnnb1 12387
Biochemical Activity Mus musculus
30 MYL6 4637
Two-hybrid Homo sapiens
31 NOTCH2 4853
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
32 TAZ  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
33 AURKA 6790
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 MAP3K2 10746
Affinity Capture-Western Homo sapiens
35 VPS51 738
Two-hybrid Homo sapiens
36 ARG1 383
Affinity Capture-MS Homo sapiens
37 DDX20 11218
Affinity Capture-MS Homo sapiens
38 CDH1 999
Co-localization Homo sapiens
39 TRIM21 6737
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
40 OGT 8473
Affinity Capture-MS Homo sapiens
41 CDK5R1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
42 ZFP91 80829
Affinity Capture-MS Homo sapiens
43 GJB5  
Two-hybrid Homo sapiens
44 SGK3 23678
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
45 CACYBP 27101
Co-fractionation Homo sapiens
46 MAP2K7 5609
Co-fractionation Homo sapiens
47 PHLPP1  
Biochemical Activity Homo sapiens
48 BIRC2  
Affinity Capture-Western Homo sapiens
49 HIST1H2AD 3013
Affinity Capture-MS Homo sapiens
50 SMG7  
Two-hybrid Homo sapiens
51 DNMT3L  
Two-hybrid Homo sapiens
52 ANXA1 301
Affinity Capture-MS Homo sapiens
53 FOXO1  
Co-localization Homo sapiens
54 PRKAR1A 5573
Affinity Capture-MS Homo sapiens
55 CD274 29126
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
56 PRKCZ 5590
Co-localization Homo sapiens
57 FZD1 8321
Co-fractionation Homo sapiens
58 STRAP 11171
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
59 GEMIN4 50628
Affinity Capture-MS Homo sapiens
60 UBR1 197131
Two-hybrid Homo sapiens
61 CSF3R  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 C11orf98  
Two-hybrid Homo sapiens
64 CREB3L3  
Biochemical Activity Homo sapiens
65 HSPA4 3308
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
66 DUSP9  
Two-hybrid Homo sapiens
67 ACTL6B  
Two-hybrid Homo sapiens
68 PELI3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
69 AKT1 207
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
70 NFATC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
71 UBXN2B  
Affinity Capture-MS Homo sapiens
72 STUB1 10273
Affinity Capture-Western Homo sapiens
73 RELA 5970
Affinity Capture-Western Homo sapiens
74 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 DHX36 170506
Affinity Capture-MS Homo sapiens
76 Gm4779  
Two-hybrid Mus musculus
77 HSF1  
Affinity Capture-Western Homo sapiens
78 DEAF1 10522
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
79 CDC37 11140
Affinity Capture-MS Homo sapiens
80 TLE1 7088
Two-hybrid Homo sapiens
81 SMN2 6607
Affinity Capture-MS Homo sapiens
82 HSP90AA1 3320
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
83 TBK1 29110
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
84 GAPDH 2597
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
85 PPP1R2 5504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 AREL1  
Affinity Capture-Western Homo sapiens
87 WSB1 26118
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
88 ENTPD6  
Two-hybrid Homo sapiens
89 IGSF21  
Two-hybrid Homo sapiens
90 ACACB 32
Affinity Capture-MS Homo sapiens
91 CEBPB  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
92 Axin1  
Two-hybrid Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Reconstituted Complex Rattus norvegicus
93 GPR39  
Two-hybrid Homo sapiens
94 DNMT1 1786
Two-hybrid Homo sapiens
95 PPARGC1A  
Biochemical Activity Homo sapiens
96 SP7  
Affinity Capture-Western Homo sapiens
97 Lzts2  
Two-hybrid Mus musculus
98 CTNND1 1500
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
99 CENPB 1059
Two-hybrid Homo sapiens
100 Krba1  
Two-hybrid Mus musculus
101 CLEC3B 7123
Two-hybrid Homo sapiens
102 LYZ 4069
Affinity Capture-MS Homo sapiens
103 NAT9  
Two-hybrid Homo sapiens
104 HMCES  
Co-fractionation Homo sapiens
105 TSC2 7249
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
106 E2F1 1869
Affinity Capture-Western Homo sapiens
107 APC  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 HSP90AB1 3326
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
109 GBP2  
Two-hybrid Homo sapiens
110 DDIT4  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
111 FRAT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 RPS18 6222
Affinity Capture-MS Homo sapiens
113 HP 3240
Affinity Capture-MS Homo sapiens
114 STK11 6794
Affinity Capture-Western Homo sapiens
115 HSPA2 3306
Affinity Capture-MS Homo sapiens
116 FAM83D 81610
Two-hybrid Homo sapiens
117 MUC1 4582
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
118 MICAL1 64780
Two-hybrid Homo sapiens
119 GGCT 79017
Affinity Capture-MS Homo sapiens
120 FIBP 9158
Two-hybrid Homo sapiens
121 U2AF1 7307
Affinity Capture-MS Homo sapiens
122 MPP1 4354
Two-hybrid Homo sapiens
123 TMEM44  
Two-hybrid Homo sapiens
124 GSK3B 2932
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
125 ARHGEF11 9826
Affinity Capture-Western Homo sapiens
126 ITLN1 55600
Affinity Capture-MS Homo sapiens
127 PFN1 5216
Affinity Capture-MS Homo sapiens
128 YBX1 4904
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
129 TEKT5  
Two-hybrid Homo sapiens
130 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
131 KRBA1  
Affinity Capture-Western Homo sapiens
132 BAG6 7917
Two-hybrid Homo sapiens
133 UBR5 51366
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
134 PMAIP1  
Two-hybrid Homo sapiens
135 GYS1 2997
Biochemical Activity Homo sapiens
136 S100A8 6279
Affinity Capture-MS Homo sapiens
137 LUC7L2 51631
Two-hybrid Homo sapiens
138 LYAR 55646
Two-hybrid Homo sapiens
139 UBXN6 80700
Two-hybrid Homo sapiens
140 TCAP  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
141 CAT 847
Affinity Capture-MS Homo sapiens
142 CASP14 23581
Affinity Capture-MS Homo sapiens
143 PTPN11 5781
Co-fractionation Homo sapiens
144 KHSRP 8570
Two-hybrid Homo sapiens
145 ACLY 47
Affinity Capture-MS Homo sapiens
146 DEFA1 1667
Two-hybrid Homo sapiens
147 SLA  
Two-hybrid Homo sapiens
148 RPS2 6187
Two-hybrid Homo sapiens
149 XPO1 7514
Affinity Capture-MS Homo sapiens
150 ILKAP 80895
Co-fractionation Homo sapiens
151 PRKAR2A 5576
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
152 SREBF1 6720
Biochemical Activity Homo sapiens
153 RSU1 6251
Two-hybrid Homo sapiens
154 SPTBN4 57731
Two-hybrid Homo sapiens
155 RICTOR 253260
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
156 RASSF1  
Affinity Capture-Western Homo sapiens
157 Prune  
Two-hybrid Mus musculus
158 PTK2 5747
Affinity Capture-Western Homo sapiens
159 PRUNE 58497
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
160 UPF3A  
Two-hybrid Homo sapiens
161 AXIN2 8313
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
162 CMTM2  
Two-hybrid Homo sapiens
163 EFTUD2 9343
Two-hybrid Homo sapiens
164 CIITA  
Biochemical Activity Homo sapiens
165 Vta1 66201
Two-hybrid Mus musculus
166 PIAS1 8554
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
167 MYC  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Synthetic Lethality Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
168 CEBPZ  
Two-hybrid Homo sapiens
169 ARC  
Affinity Capture-Western Homo sapiens
170 RAI1 10743
Two-hybrid Homo sapiens
171 LMNA 4000
Proximity Label-MS Homo sapiens
172 DMBT1 1755
Affinity Capture-MS Homo sapiens
173 RPL13 6137
Affinity Capture-MS Homo sapiens
174 C10orf90 118611
Two-hybrid Homo sapiens
175 SMURF2 64750
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
176 CSAD 51380
Two-hybrid Homo sapiens
177 LTF 4057
Affinity Capture-MS Homo sapiens
178 TRAF2 7186
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 PARP1 142
Proximity Label-MS Homo sapiens
180 ACSBG1  
Two-hybrid Homo sapiens
181 TP53 7157
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
182 CLASP2 23122
Affinity Capture-MS Homo sapiens
183 HDAC6 10013
Affinity Capture-Western Homo sapiens
184 SMAD1 4086
Co-localization Homo sapiens
185 SQSTM1 8878
Co-localization Homo sapiens
186 PRKRIP1 79706
Two-hybrid Homo sapiens
187 TSC1 7248
Affinity Capture-Western Homo sapiens
188 PRKACA 5566
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
189 ZFPM1 161882
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
190 TRIP12 9320
Affinity Capture-MS Homo sapiens
191 KLF5  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
192 DBI 1622
Two-hybrid Homo sapiens
193 PRKCA 5578
Biochemical Activity Homo sapiens
194 SSSCA1 10534
Affinity Capture-MS Homo sapiens
195 BCCIP 56647
Two-hybrid Homo sapiens
196 CABYR  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
197 CDX2  
Affinity Capture-Western Homo sapiens
198 XPNPEP1 7511
Two-hybrid Homo sapiens
199 PFKFB4 5210
Two-hybrid Homo sapiens
200 PPP3CA 5530
Affinity Capture-MS Homo sapiens
201 SERPINB4 6318
Affinity Capture-MS Homo sapiens
202 EEF1G 1937
Two-hybrid Homo sapiens
203 Arhgef11  
Two-hybrid Mus musculus
204 AKAP11 11215
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
205 IGHM 3507
Two-hybrid Homo sapiens
206 FRAT1 10023
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
207 MED24 9862
Two-hybrid Homo sapiens
208 PTPN1 5770
Biochemical Activity Homo sapiens
209 SOX9  
Biochemical Activity Homo sapiens
210 SOX10  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
211 ACR  
Affinity Capture-MS Homo sapiens
212 NFE2L2 4780
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
213 EWSR1 2130
Affinity Capture-MS Homo sapiens
214 PSMC2 5701
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
215 NUB1 51667
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
216 RCAN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
217 Zbed3  
Two-hybrid Mus musculus
218 CSNK2B 1460
Two-hybrid Homo sapiens
219 RBPJ 3516
Two-hybrid Homo sapiens
220 FEN1 2237
Two-hybrid Homo sapiens
221 SAMHD1 25939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
222 PSME3 10197
Affinity Capture-Western Homo sapiens
223 BRIX1 55299
Two-hybrid Homo sapiens
224 EPB41L3 23136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
225 ADAP1  
Two-hybrid Homo sapiens
226 IQCG 84223
Two-hybrid Homo sapiens
227 NBR1 4077
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
228 TUBA1A 7846
Two-hybrid Homo sapiens
229 SNAI2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
230 Dvl1  
Phenotypic Suppression Mus musculus
231 MAPT  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
232 MUC5AC 4586
Affinity Capture-MS Homo sapiens
233 MID1IP1  
Two-hybrid Homo sapiens
234 RPLP1 6176
Two-hybrid Homo sapiens
235 APBA1  
Affinity Capture-MS Homo sapiens
236 HIST1H2BK 85236
Affinity Capture-MS Homo sapiens
237 EIF4EBP1  
Two-hybrid Homo sapiens
238 ATF3  
Affinity Capture-Western Homo sapiens
239 DNM1L 10059
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
240 TONSL  
Two-hybrid Homo sapiens
241 DCTN3 11258
Two-hybrid Homo sapiens
242 KIAA1191  
Two-hybrid Homo sapiens
243 MASP1 5648
Two-hybrid Homo sapiens
244 ZHX1  
Two-hybrid Homo sapiens
245 CARTPT  
Affinity Capture-MS Homo sapiens
246 TRAF6 7189
Affinity Capture-Western Homo sapiens
247 PDE4D  
Biochemical Activity Homo sapiens
248 SNCAIP  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
249 H3F3B 3021
Affinity Capture-MS Homo sapiens
250 PIM2  
Two-hybrid Homo sapiens
251 NRBP1 29959
Two-hybrid Homo sapiens
252 EPM2A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
253 GNB2 2783
Two-hybrid Homo sapiens
254 MTOR 2475
Two-hybrid Homo sapiens
255 RCAN2  
Affinity Capture-MS Homo sapiens
256 HAX1  
Affinity Capture-MS Homo sapiens
257 TRAK1 22906
Affinity Capture-MS Homo sapiens
258 UBE2D1 7321
Two-hybrid Homo sapiens
259 ZNF227  
Two-hybrid Homo sapiens
260 FZD5 7855
Two-hybrid Homo sapiens
261 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
262 FBXW11  
Two-hybrid Homo sapiens
263 GIPC1 10755
Two-hybrid Homo sapiens
264 Spice1  
Two-hybrid Mus musculus
265 RPL6 6128
Affinity Capture-MS Homo sapiens
266 TSKS  
Affinity Capture-MS Homo sapiens
267 IKBKG 8517
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
268 EDEM1  
Affinity Capture-MS Homo sapiens
269 HNRNPM 4670
Two-hybrid Homo sapiens
270 ANKRD6  
Affinity Capture-Western Homo sapiens
271 TRIM25 7706
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
272 FKBP14  
Two-hybrid Homo sapiens
273 TLR9  
Affinity Capture-MS Homo sapiens
274 KDM1A 23028
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
275 PRSS37  
Affinity Capture-MS Homo sapiens
276 ZG16B 124220
Affinity Capture-MS Homo sapiens
277 Nbr1  
Two-hybrid Mus musculus
278 FOXM1  
Affinity Capture-Western Homo sapiens
279 UHRF1 29128
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
280 AR 367
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
281 RPL36AL 6166
Two-hybrid Homo sapiens
282 PRKCB 5579
Co-localization Homo sapiens
283 ATXN3 4287
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
284 VIM 7431
Two-hybrid Homo sapiens
285 NOTCH1 4851
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
286 CRBN  
Affinity Capture-Western Homo sapiens
287 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
288 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
289 ATPIF1 93974
Two-hybrid Homo sapiens
290 XIAP  
Affinity Capture-Western Homo sapiens
291 FBN3 84467
Two-hybrid Homo sapiens
292 QARS 5859
Two-hybrid Homo sapiens
293 STAT2 6773
Biochemical Activity Homo sapiens
294 DCP1B  
Two-hybrid Homo sapiens
295 MKL1 57591
Affinity Capture-Western Homo sapiens
296 BZW2 28969
Two-hybrid Homo sapiens
297 PTN  
Two-hybrid Homo sapiens
298 DEC1  
Two-hybrid Homo sapiens
299 MPO 4353
Affinity Capture-MS Homo sapiens
300 FBXL21  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
301 GFI1  
Biochemical Activity Homo sapiens
302 APP 351
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
303 GSK3A 2931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
304 PPP1CC 5501
Affinity Capture-MS Homo sapiens
305 TOP2A 7153
Affinity Capture-Western Homo sapiens
306 LRP6 4040
Far Western Homo sapiens
Biochemical Activity Homo sapiens
307 KIF5B 3799
Two-hybrid Homo sapiens
308 NUFIP1  
Affinity Capture-MS Homo sapiens
309 COPS5 10987
Affinity Capture-Western Homo sapiens
310 FBXW7  
Affinity Capture-Western Homo sapiens
311 CCDC6 8030
Affinity Capture-Western Homo sapiens
312 Nono 53610
Two-hybrid Mus musculus
313 CHD3 1107
Two-hybrid Homo sapiens
314 ASRGL1 80150
Two-hybrid Homo sapiens
315 LMO4  
Two-hybrid Homo sapiens
316 H2-Aa  
Two-hybrid Mus musculus
317 MAPK1 5594
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
318 PPP1CA 5499
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
319 BHLHE41  
Two-hybrid Homo sapiens
320 CST6  
Two-hybrid Homo sapiens
321 CTSG 1511
Affinity Capture-MS Homo sapiens
322 SMAD3 4088
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
323 BPIFB1 92747
Affinity Capture-MS Homo sapiens
324 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
325 EYA1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
326 BIRC3 330
Affinity Capture-Western Homo sapiens
327 DKK3 27122
Affinity Capture-MS Homo sapiens
328 YBX3 8531
Biochemical Activity Homo sapiens
329 CASC4 113201
Two-hybrid Homo sapiens
330 IBTK 25998
Affinity Capture-Western Homo sapiens
331 PSEN1 5663
Biochemical Activity Homo sapiens
332 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
333 C14orf1 11161
Two-hybrid Homo sapiens
334 Axin1  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
335 CAMSAP3  
Two-hybrid Homo sapiens
336 PSMD8 5714
Two-hybrid Homo sapiens
337 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
338 RELB  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
339 SAP30BP  
Two-hybrid Homo sapiens
340 CCNE1  
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
341 MAP3K1 4214
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
342 BCL3  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
343 TRIB3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
344 Map3k11  
Two-hybrid Mus musculus
345 GDI2 2665
Co-fractionation Homo sapiens
346 XPO6 23214
Affinity Capture-MS Homo sapiens
347 JUN 3725
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
348 FKBP5 2289
Affinity Capture-MS Homo sapiens
349 NIN 51199
Two-hybrid Homo sapiens
350 MAP3K4 4216
Biochemical Activity Homo sapiens
351 SAMD9 54809
Affinity Capture-Western Homo sapiens
352 AKT2 208
Co-crystal Structure Homo sapiens
353 DHX34 9704
Two-hybrid Homo sapiens
354 NFKB1 4790
Biochemical Activity Homo sapiens
355 DYRK2 8445
Affinity Capture-Western Homo sapiens
356 RNF114 55905
Affinity Capture-MS Homo sapiens
357 CCND1 595
Co-localization Homo sapiens
358 MDM2  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
359 MGEA5 10724
Two-hybrid Homo sapiens
360 Prox2  
Two-hybrid Mus musculus
361 DNAJC13 23317
Two-hybrid Homo sapiens
362 GSN 2934
Affinity Capture-MS Homo sapiens
363 SNCA 6622
Reconstituted Complex Homo sapiens
364 SPICE1  
Affinity Capture-Western Homo sapiens
365 RNF220  
Affinity Capture-Western Homo sapiens
366 SNAI1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
367 ZNF746  
Two-hybrid Homo sapiens
368 RPS15 6209
Two-hybrid Homo sapiens
369 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
370 GSKIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
371 VTA1 51534
Affinity Capture-Western Homo sapiens
372 CREB1  
Biochemical Activity Homo sapiens
373 AXIN1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
374 SMYD2 56950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
375 ARRB1 408
Biochemical Activity Homo sapiens
376 RXRA 6256
Two-hybrid Homo sapiens
377 MAP3K11 4296
Affinity Capture-Western Homo sapiens
378 BEX1  
Two-hybrid Homo sapiens
379 PPP3CC 5533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
380 NFE2L3 9603
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
381 HECW2  
Affinity Capture-MS Homo sapiens
382 UFM1 51569
Two-hybrid Homo sapiens
383 ARIH1 25820
Affinity Capture-MS Homo sapiens
384 Deaf1  
Two-hybrid Mus musculus
385 ZNF135  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GSK3B is involved
PathwayEvidenceSource
AKT phosphorylates targets in the cytosol IEA Reactome
APC truncation mutants have impaired AXIN binding TAS Reactome
AXIN missense mutants destabilize the destruction complex TAS Reactome
Axon guidance TAS Reactome
B-WICH complex positively regulates rRNA expression TAS Reactome
Beta-catenin phosphorylation cascade TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to chemical stress TAS Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Constitutive Signaling by AKT1 E17K in Cancer TAS Reactome
CRMPs in Sema3A signaling TAS Reactome
CTNNB1 S33 mutants aren't phosphorylated TAS Reactome
CTNNB1 S37 mutants aren't phosphorylated TAS Reactome
CTNNB1 S45 mutants aren't phosphorylated TAS Reactome
CTNNB1 T41 mutants aren't phosphorylated TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
Degradation of GLI2 by the proteasome IEA Reactome
Developmental Biology TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Epigenetic regulation of gene expression TAS Reactome
Gene expression (Transcription) IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome TAS Reactome
GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 TAS Reactome
Hedgehog 'off' state TAS Reactome
Hedgehog 'off' state IEA Reactome
Infectious disease TAS Reactome
Intracellular signaling by second messengers IEA Reactome
KEAP1-NFE2L2 pathway TAS Reactome
Late SARS-CoV-2 Infection Events TAS Reactome
Maturation of nucleoprotein TAS Reactome
Maturation of nucleoprotein TAS Reactome
MITF-M-regulated melanocyte development TAS Reactome
Nervous system development TAS Reactome
Nuclear events mediated by NFE2L2 TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PIP3 activates AKT signaling IEA Reactome
Positive epigenetic regulation of rRNA expression TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Regulation of RUNX2 expression and activity IEA Reactome
RNA Polymerase II Transcription IEA Reactome
S Phase IEA Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-2 Infection TAS Reactome
Semaphorin interactions TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by AMER1 mutants TAS Reactome
Signaling by APC mutants TAS Reactome
Signaling by AXIN mutants TAS Reactome
Signaling by CTNNB1 phospho-site mutants TAS Reactome
Signaling by GSK3beta mutants TAS Reactome
Signaling by Hedgehog TAS Reactome
Signaling by Hedgehog IEA Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
Signaling by WNT in cancer TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
TCF dependent signaling in response to WNT IEA Reactome
Transcriptional and post-translational regulation of MITF-M expression and activity TAS Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Translation of Structural Proteins TAS Reactome
Translation of Structural Proteins TAS Reactome
Truncations of AMER1 destabilize the destruction complex TAS Reactome
Ubiquitin-dependent degradation of Cyclin D IEA Reactome
Viral Infection Pathways TAS Reactome





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