Gene description for NFE2L3
Gene name nuclear factor, erythroid 2-like 3
Gene symbol NFE2L3
Other names/aliases NRF3
Species Homo sapiens
 Database cross references - NFE2L3
ExoCarta ExoCarta_9603
Vesiclepedia VP_9603
Entrez Gene 9603
HGNC 7783
MIM 604135
UniProt Q9Y4A8  
 NFE2L3 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for NFE2L3
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IEA
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 NAS
    transcription by RNA polymerase II GO:0006366 TAS
    integrated stress response signaling GO:0140467 NAS
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 NAS
    RNA polymerase II transcription regulator complex GO:0090575 IPI
 Experiment description of studies that identified NFE2L3 in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for NFE2L3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FBXW7  
Affinity Capture-Western Homo sapiens
2 PPARG 5468
Protein-peptide Homo sapiens
3 CREB3  
FRET Homo sapiens
4 NFE2  
FRET Homo sapiens
FRET Homo sapiens
5 NFE2L2 4780
FRET Homo sapiens
6 MAFK 7975
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HCFC1 3054
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MAFF 23764
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
9 NFE2L3 9603
FRET Homo sapiens
FRET Homo sapiens
10 HCFC2  
Affinity Capture-MS Homo sapiens
11 NOC3L 64318
Cross-Linking-MS (XL-MS) Homo sapiens
12 GSK3B 2932
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
13 MAFG  
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 NFE2L1  
FRET Homo sapiens
15 PSCA 8000
Affinity Capture-MS Homo sapiens
16 BACH2 60468
FRET Homo sapiens
View the network image/svg+xml
 Pathways in which NFE2L3 is involved
No pathways found





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