Gene description for PRKACA
Gene name protein kinase, cAMP-dependent, catalytic, alpha
Gene symbol PRKACA
Other names/aliases PKACA
PPNAD4
Species Homo sapiens
 Database cross references - PRKACA
ExoCarta ExoCarta_5566
Vesiclepedia VP_5566
Entrez Gene 5566
HGNC 9380
MIM 601639
UniProt P17612  
 PRKACA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for PRKACA
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 IMP
    protein serine/threonine kinase activity GO:0004674 TAS
    cAMP-dependent protein kinase activity GO:0004691 IBA
    cAMP-dependent protein kinase activity GO:0004691 IDA
    cAMP-dependent protein kinase activity GO:0004691 ISS
    cAMP-dependent protein kinase activity GO:0004691 NAS
    cAMP-dependent protein kinase activity GO:0004691 TAS
    protein serine/threonine/tyrosine kinase activity GO:0004712 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IPI
    protein domain specific binding GO:0019904 IEA
    manganese ion binding GO:0030145 IEA
    ubiquitin protein ligase binding GO:0031625 IDA
    protein kinase A regulatory subunit binding GO:0034237 IBA
    protein kinase A regulatory subunit binding GO:0034237 IPI
    channel activator activity GO:0099103 TAS
    protein serine kinase activity GO:0106310 IDA
Biological Process
    mesoderm formation GO:0001707 IEA
    neural tube closure GO:0001843 IEA
    regulation of heart rate GO:0002027 TAS
    renal water homeostasis GO:0003091 TAS
    mRNA processing GO:0006397 IDA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 NAS
    protein export from nucleus GO:0006611 IEA
    signal transduction GO:0007165 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IEA
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 IDA
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 TAS
    regulation of macroautophagy GO:0016241 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-serine phosphorylation GO:0018105 IMP
    cytokine-mediated signaling pathway GO:0019221 TAS
    positive regulation of insulin secretion GO:0032024 IEA
    negative regulation of interleukin-2 production GO:0032703 ISS
    high-density lipoprotein particle assembly GO:0034380 TAS
    cellular response to heat GO:0034605 IDA
    mitochondrial protein catabolic process GO:0035694 TAS
    positive regulation of cholesterol biosynthetic process GO:0045542 IDA
    regulation of osteoblast differentiation GO:0045667 IDA
    positive regulation of gluconeogenesis GO:0045722 IEA
    negative regulation of smoothened signaling pathway GO:0045879 IEA
    positive regulation of protein export from nucleus GO:0046827 IEA
    sperm capacitation GO:0048240 ISS
    positive regulation of calcium-mediated signaling GO:0050850 TAS
    regulation of cell cycle GO:0051726 ISS
    regulation of cardiac muscle contraction GO:0055117 TAS
    regulation of proteasomal protein catabolic process GO:0061136 IDA
    cellular response to cold GO:0070417 IEA
    regulation of protein processing GO:0070613 IEA
    cellular response to glucose stimulus GO:0071333 IDA
    cellular response to parathyroid hormone stimulus GO:0071374 IEA
    cellular response to glucagon stimulus GO:0071377 IEA
    cellular response to epinephrine stimulus GO:0071872 TAS
    cell communication by electrical coupling involved in cardiac conduction GO:0086064 TAS
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IEA
    cAMP/PKA signal transduction GO:0141156 IDA
    regulation of cardiac conduction GO:1903779 TAS
    negative regulation of TORC1 signaling GO:1904262 IDA
    negative regulation of glycolytic process through fructose-6-phosphate GO:1904539 IEA
    protein localization to lipid droplet GO:1990044 IEA
    regulation of bicellular tight junction assembly GO:2000810 IDA
Subcellular Localization
    acrosomal vesicle GO:0001669 ISS
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IC
    cytoplasm GO:0005737 IDA
    mitochondrial matrix GO:0005759 TAS
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    axoneme GO:0005930 IDA
    cAMP-dependent protein kinase complex GO:0005952 IBA
    cAMP-dependent protein kinase complex GO:0005952 IDA
    cAMP-dependent protein kinase complex GO:0005952 NAS
    nuclear speck GO:0016607 IDA
    nucleotide-activated protein kinase complex GO:0031588 IDA
    neuromuscular junction GO:0031594 IEA
    calcium channel complex GO:0034704 TAS
    sperm flagellum GO:0036126 IEA
    plasma membrane raft GO:0044853 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
    sperm midpiece GO:0097225 IDA
    ciliary base GO:0097546 TAS
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified PRKACA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
21
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
22
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
25
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
28
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
31
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
35
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
38
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
39
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
41
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKACA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 KRT77 374454
Affinity Capture-MS Homo sapiens
3 CSE1L 1434
Affinity Capture-MS Homo sapiens
4 SLC25A13 10165
Affinity Capture-MS Homo sapiens
5 GTF2F1 2962
Cross-Linking-MS (XL-MS) Homo sapiens
6 PCNT  
Affinity Capture-MS Homo sapiens
7 ITGA2B 3674
Biochemical Activity Homo sapiens
8 GABRB3 2562
Reconstituted Complex Homo sapiens
9 HNRNPM 4670
Co-fractionation Homo sapiens
10 Psmb5 19173
Affinity Capture-MS Mus musculus
11 ARPC5L 81873
Co-fractionation Homo sapiens
12 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MARCKS 4082
Co-fractionation Homo sapiens
14 NCS1 23413
Affinity Capture-MS Homo sapiens
15 LLGL1 3996
Proximity Label-MS Homo sapiens
16 AKAP12 9590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PDE7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GPR161  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SH3GL1 6455
Co-fractionation Homo sapiens
20 GSTM2 2946
Affinity Capture-MS Homo sapiens
21 RPA2 6118
Co-fractionation Homo sapiens
22 AKAP11 11215
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 JPH1 56704
Proximity Label-MS Homo sapiens
24 DNAJA1 3301
Affinity Capture-MS Homo sapiens
25 RRAD 6236
Biochemical Activity Homo sapiens
26 RASAL2 9462
Proximity Label-MS Homo sapiens
27 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
28 DACT1 51339
Affinity Capture-Western Homo sapiens
29 PRKACG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 UBE3A 7337
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
31 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CCT4 10575
Affinity Capture-MS Homo sapiens
33 EIF4A1 1973
Affinity Capture-MS Homo sapiens
34 BASP1 10409
Proximity Label-MS Homo sapiens
35 TRIM55  
Two-hybrid Homo sapiens
36 PPP1R10  
Biochemical Activity Homo sapiens
37 PARK7 11315
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
38 UBE2N 7334
Affinity Capture-MS Homo sapiens
39 SNX6 58533
Proximity Label-MS Homo sapiens
40 SLC12A2 6558
Proximity Label-MS Homo sapiens
41 KDELR1 10945
Biochemical Activity Homo sapiens
42 DNAJB6 10049
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 GSK3A 2931
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
44 DIAPH3 81624
Proximity Label-MS Homo sapiens
45 DEPDC1B 55789
Proximity Label-MS Homo sapiens
46 CAPZB 832
Affinity Capture-MS Homo sapiens
47 VRK2 7444
Proximity Label-MS Homo sapiens
48 FIS1 51024
Proximity Label-MS Homo sapiens
49 VANGL2  
Proximity Label-MS Homo sapiens
50 YWHAZ 7534
Affinity Capture-MS Homo sapiens
51 DPM1 8813
Affinity Capture-MS Homo sapiens
52 STEAP3 55240
Proximity Label-MS Homo sapiens
53 USO1 8615
Affinity Capture-MS Homo sapiens
54 DYRK1A 1859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PKIA  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
56 ZNF724P  
Affinity Capture-MS Homo sapiens
57 ASIC1  
Biochemical Activity Homo sapiens
58 CALD1 800
Biochemical Activity Homo sapiens
59 TMEM231 79583
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
60 TIMM50 92609
Affinity Capture-MS Homo sapiens
61 MEF2D  
Biochemical Activity Homo sapiens
62 IQGAP1 8826
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
63 STX7 8417
Proximity Label-MS Homo sapiens
64 TNIK 23043
Proximity Label-MS Homo sapiens
65 SLC25A12 8604
Affinity Capture-MS Homo sapiens
66 MAP4K5 11183
Proximity Label-MS Homo sapiens
67 TRAP1 10131
Affinity Capture-MS Homo sapiens
68 TNP2  
Biochemical Activity Homo sapiens
69 AGK 55750
Affinity Capture-MS Homo sapiens
70 MAP2 4133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 MAP3K3 4215
Co-localization Homo sapiens
72 ABCE1 6059
Affinity Capture-MS Homo sapiens
73 LARP1B 55132
Affinity Capture-MS Homo sapiens
74 CRYBG3  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SIK3 23387
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
77 EXD2  
Proximity Label-MS Homo sapiens
78 MARCKSL1 65108
Proximity Label-MS Homo sapiens
79 AIFM1 9131
Affinity Capture-MS Homo sapiens
80 TUBA3C 7278
Affinity Capture-MS Homo sapiens
81 ESPL1  
Proximity Label-MS Homo sapiens
82 SNAP23 8773
Proximity Label-MS Homo sapiens
83 GTSE1 51512
Affinity Capture-MS Homo sapiens
84 CALR 811
Co-fractionation Homo sapiens
85 MAVS 57506
Affinity Capture-Western Homo sapiens
86 BTRC 8945
Affinity Capture-MS Homo sapiens
87 FTSJ1 24140
Proximity Label-MS Homo sapiens
88 DDX17 10521
Affinity Capture-MS Homo sapiens
89 PLS3 5358
Co-fractionation Homo sapiens
90 SLU7  
Affinity Capture-MS Homo sapiens
91 AKIP1  
Reconstituted Complex Homo sapiens
92 PABPC4 8761
Affinity Capture-MS Homo sapiens
93 SIK2  
Affinity Capture-MS Homo sapiens
94 GSK3B 2932
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
95 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
96 FGF2 2247
Affinity Capture-Western Homo sapiens
97 DYRK1B  
Affinity Capture-MS Homo sapiens
98 CCT2 10576
Affinity Capture-MS Homo sapiens
99 VTN 7448
Biochemical Activity Homo sapiens
100 CAND1 55832
Affinity Capture-MS Homo sapiens
101 PPP1CA 5499
Co-fractionation Homo sapiens
102 RELA 5970
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
103 R3HCC1L  
Proximity Label-MS Homo sapiens
104 MTCH2 23788
Proximity Label-MS Homo sapiens
105 LARP1 23367
Affinity Capture-MS Homo sapiens
106 CPNE8 144402
Proximity Label-MS Homo sapiens
107 ARPC2 10109
Co-fractionation Homo sapiens
108 TOP3B 8940
Proximity Label-MS Homo sapiens
109 EMD 2010
Affinity Capture-MS Homo sapiens
110 SRP14 6727
Proximity Label-MS Homo sapiens
111 KLHL3  
Biochemical Activity Homo sapiens
112 IRF2 3660
Biochemical Activity Homo sapiens
113 ERBB3 2065
Biochemical Activity Homo sapiens
114 STOML2 30968
Affinity Capture-MS Homo sapiens
115 Grin2c  
Biochemical Activity Rattus norvegicus
116 OCLN 100506658
Proximity Label-MS Homo sapiens
117 PSMD11 5717
Biochemical Activity Homo sapiens
118 MAPRE1 22919
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 CCT5 22948
Affinity Capture-MS Homo sapiens
120 RPE  
Co-fractionation Homo sapiens
121 TPD52 7163
Co-fractionation Homo sapiens
122 PDE3A  
Biochemical Activity Homo sapiens
123 C5orf30  
Affinity Capture-MS Homo sapiens
124 MAPT  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
125 PABPC1 26986
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 AHCY 191
Affinity Capture-MS Homo sapiens
127 FYN 2534
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
128 PARD3 56288
Proximity Label-MS Homo sapiens
129 MYH9 4627
Affinity Capture-MS Homo sapiens
130 PPP1R8 5511
Biochemical Activity Homo sapiens
131 TRIM21 6737
Affinity Capture-MS Homo sapiens
132 AKAP9 10142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
133 HAND1  
Biochemical Activity Homo sapiens
134 DCAF7 10238
Affinity Capture-MS Homo sapiens
135 IFI16 3428
Affinity Capture-MS Homo sapiens
136 NCL 4691
Co-fractionation Homo sapiens
137 NXPE4 54827
Affinity Capture-MS Homo sapiens
138 TRIP10 9322
Biochemical Activity Homo sapiens
139 GAPDH 2597
Co-fractionation Homo sapiens
140 CCDC8  
Proximity Label-MS Homo sapiens
141 APOBEC3G  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
142 Bag2  
Affinity Capture-MS Mus musculus
143 AKAP7 9465
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 MYH10 4628
Affinity Capture-MS Homo sapiens
145 PRKDC 5591
Affinity Capture-MS Homo sapiens
146 ANXA1 301
Affinity Capture-MS Homo sapiens
147 ARPC5 10092
Co-fractionation Homo sapiens
148 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
149 PRKAR1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 Cdk5rap2  
Affinity Capture-MS Mus musculus
151 ITCH 83737
Biochemical Activity Homo sapiens
152 RHOT2 89941
Proximity Label-MS Homo sapiens
153 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
154 CAMKK2 10645
Biochemical Activity Homo sapiens
155 TUBAL3 79861
Affinity Capture-MS Homo sapiens
156 PRKACB 5567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
157 ATP5B 506
Affinity Capture-MS Homo sapiens
158 TRIM63  
Two-hybrid Homo sapiens
159 RMDN3 55177
Proximity Label-MS Homo sapiens
160 MGMT 4255
Biochemical Activity Homo sapiens
161 CDK17 5128
Affinity Capture-MS Homo sapiens
162 CAD 790
Affinity Capture-MS Homo sapiens
163 IMMT 10989
Affinity Capture-MS Homo sapiens
164 PLEKHA5 54477
Proximity Label-MS Homo sapiens
165 smo  
Biochemical Activity Drosophila melanogaster
166 UFD1L 7353
Affinity Capture-Western Homo sapiens
167 SLC4A7 9497
Proximity Label-MS Homo sapiens
168 TOR1AIP1 26092
Proximity Label-MS Homo sapiens
169 SMCR8 140775
Proximity Label-MS Homo sapiens
170 FAM83B  
Proximity Label-MS Homo sapiens
171 CYP3A4  
Biochemical Activity Homo sapiens
172 ITPR2 3709
Proximity Label-MS Homo sapiens
173 NFAT5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
174 LARP4 113251
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
175 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
176 NASP 4678
Co-fractionation Homo sapiens
177 WDR41  
Proximity Label-MS Homo sapiens
178 KIAA1244  
Biochemical Activity Homo sapiens
179 XPO1 7514
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 CDCA3 83461
Proximity Label-MS Homo sapiens
181 AKAP14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
182 CLPP 8192
Proximity Label-MS Homo sapiens
183 VDAC2 7417
Affinity Capture-MS Homo sapiens
184 CCT6A 908
Affinity Capture-MS Homo sapiens
185 UBQLN2 29978
Co-fractionation Homo sapiens
186 FERMT2 10979
Proximity Label-MS Homo sapiens
187 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
188 FOXO1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
189 PRKAR1A 5573
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
190 DOCK1 1793
Biochemical Activity Homo sapiens
191 CUL3 8452
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
192 PDE4D  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
193 CKAP2  
Proximity Label-MS Homo sapiens
194 AKAP13 11214
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
195 FKBP8 23770
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
196 HSPA8 3312
Co-fractionation Homo sapiens
197 FUS 2521
Affinity Capture-MS Homo sapiens
198 MAP7 9053
Proximity Label-MS Homo sapiens
199 PDPK1 5170
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
200 TP73  
Affinity Capture-Western Homo sapiens
201 HNRNPCL1 343069
Affinity Capture-MS Homo sapiens
202 TNP1  
Biochemical Activity Homo sapiens
203 OCIAD1 54940
Proximity Label-MS Homo sapiens
204 NXF1 10482
Affinity Capture-RNA Homo sapiens
205 GRAMD1A  
Proximity Label-MS Homo sapiens
206 CDK5RAP2 55755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
207 PTPN7 5778
Biochemical Activity Homo sapiens
208 RANBP9 10048
Two-hybrid Homo sapiens
209 FKBP5 2289
Affinity Capture-MS Homo sapiens
210 RGS13  
Biochemical Activity Homo sapiens
211 ASIC3  
Biochemical Activity Homo sapiens
212 AKAP2 11217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
213 PPIL3 53938
Affinity Capture-MS Homo sapiens
214 Uso1 56041
Affinity Capture-MS Mus musculus
215 NIN 51199
Biochemical Activity Homo sapiens
216 LMNB1 4001
Affinity Capture-MS Homo sapiens
217 AKAP5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
218 GCN1L1 10985
Affinity Capture-MS Homo sapiens
219 EGFR 1956
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
220 CCT8 10694
Affinity Capture-MS Homo sapiens
221 HAND2  
Biochemical Activity Homo sapiens
222 MICALL1 85377
Proximity Label-MS Homo sapiens
223 FGG 2266
Affinity Capture-MS Homo sapiens
224 GSTM1 2944
Affinity Capture-MS Homo sapiens
225 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
226 PPFIBP1 8496
Proximity Label-MS Homo sapiens
227 PALM2-AKAP2 445815
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
228 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
229 DDRGK1 65992
Proximity Label-MS Homo sapiens
230 CLCC1 23155
Proximity Label-MS Homo sapiens
231 SBF1 6305
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
232 VDAC1 7416
Affinity Capture-MS Homo sapiens
233 CLTC 1213
Two-hybrid Homo sapiens
234 CCDC88A 55704
Two-hybrid Homo sapiens
235 HSPD1 3329
Co-fractionation Homo sapiens
236 HSPA9 3313
Co-fractionation Homo sapiens
237 COLGALT1 79709
Co-fractionation Homo sapiens
238 LMNA 4000
Proximity Label-MS Homo sapiens
239 RASGRP3  
Biochemical Activity Homo sapiens
240 Prkar2a 19087
Affinity Capture-MS Mus musculus
241 ARL13B 200894
Proximity Label-MS Homo sapiens
242 BAIAP2 10458
Proximity Label-MS Homo sapiens
243 AKAP1 8165
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
244 Prkacb 18749
Affinity Capture-MS Mus musculus
245 HUWE1 10075
Affinity Capture-MS Homo sapiens
246 PHGDH 26227
Affinity Capture-MS Homo sapiens
247 ARPC1A 10552
Co-fractionation Homo sapiens
248 PFKFB1  
Biochemical Activity Homo sapiens
249 TUBB2A 7280
Affinity Capture-MS Homo sapiens
250 MARK3 4140
Proximity Label-MS Homo sapiens
251 ARPC3 10094
Co-fractionation Homo sapiens
252 CEP89 84902
Proximity Label-MS Homo sapiens
253 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
254 FBXW11  
Two-hybrid Homo sapiens
255 DYNLL2 140735
Affinity Capture-MS Homo sapiens
256 GAD2  
Biochemical Activity Homo sapiens
257 PKIG  
Affinity Capture-MS Homo sapiens
258 AKR1B1 231
Co-fractionation Homo sapiens
259 USP49 25862
Affinity Capture-MS Homo sapiens
260 MAST3  
Affinity Capture-MS Homo sapiens
261 BAD  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
262 SNTB1 6641
Proximity Label-MS Homo sapiens
263 ROR2 4920
Proximity Label-MS Homo sapiens
264 HMMR  
Proximity Label-MS Homo sapiens
265 PGRMC1 10857
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
266 TUBG1 7283
Affinity Capture-MS Homo sapiens
267 TRMT10A 93587
Co-fractionation Homo sapiens
268 IPO5 3843
Affinity Capture-MS Homo sapiens
269 ATAD3B 83858
Affinity Capture-MS Homo sapiens
270 LSR 51599
Proximity Label-MS Homo sapiens
271 CALU 813
Co-fractionation Homo sapiens
272 RYR2 6262
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
273 AKAP8  
Affinity Capture-MS Homo sapiens
274 ITPR3 3710
Proximity Label-MS Homo sapiens
275 GAD1  
Biochemical Activity Homo sapiens
276 RDX 5962
Co-fractionation Homo sapiens
277 RPS18 6222
Affinity Capture-MS Homo sapiens
278 CDK18 5129
Affinity Capture-MS Homo sapiens
279 DNAJA3 9093
Co-fractionation Homo sapiens
280 TUBB3 10381
Affinity Capture-MS Homo sapiens
281 AICDA  
Affinity Capture-Western Homo sapiens
282 GNA13 10672
Biochemical Activity Homo sapiens
283 ELAVL1 1994
Affinity Capture-MS Homo sapiens
284 HDAC1 3065
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
285 UGDH 7358
Co-fractionation Homo sapiens
286 KCNQ1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
287 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
288 CSNK1E 1454
Proximity Label-MS Homo sapiens
289 NUP35 129401
Proximity Label-MS Homo sapiens
290 CREB1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
291 DDX58 23586
Affinity Capture-RNA Homo sapiens
292 PRKAR2B 5577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
293 AARSD1 80755
Co-fractionation Homo sapiens
294 HDAC8 55869
Biochemical Activity Homo sapiens
295 POU2F1 5451
Biochemical Activity Homo sapiens
296 NRBP1 29959
Proximity Label-MS Homo sapiens
297 ATAD3A 55210
Affinity Capture-MS Homo sapiens
298 PDZD8 118987
Proximity Label-MS Homo sapiens
299 RPS3A 6189
Affinity Capture-MS Homo sapiens
300 XPO5 57510
Affinity Capture-MS Homo sapiens
301 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
302 MOV10 4343
Affinity Capture-RNA Homo sapiens
303 Mapre1 13589
Affinity Capture-MS Mus musculus
304 HNRNPL 3191
Co-fractionation Homo sapiens
305 ARHGAP36  
Affinity Capture-MS Homo sapiens
306 MARK2 2011
Proximity Label-MS Homo sapiens
307 Prkar2b 19088
Affinity Capture-MS Mus musculus
308 SH3GLB1 51100
Co-fractionation Homo sapiens
309 HK1 3098
Affinity Capture-MS Homo sapiens
310 UBXN4 23190
Proximity Label-MS Homo sapiens
311 TSC22D4 81628
Proximity Label-MS Homo sapiens
312 SLC6A8 6535
Proximity Label-MS Homo sapiens
313 RPS6 6194
Affinity Capture-MS Homo sapiens
314 IGF2R 3482
Proximity Label-MS Homo sapiens
315 ITPR1 3708
Proximity Label-MS Homo sapiens
316 EPHA2 1969
Proximity Label-MS Homo sapiens
317 ALDOA 226
Affinity Capture-MS Homo sapiens
318 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
319 PIK3R2 5296
Proximity Label-MS Homo sapiens
320 LMOD3  
Cross-Linking-MS (XL-MS) Homo sapiens
321 ATP5C1 509
Affinity Capture-MS Homo sapiens
322 UHRF1 29128
Biochemical Activity Homo sapiens
323 EIF5 1983
Affinity Capture-MS Homo sapiens
324 YWHAE 7531
Affinity Capture-MS Homo sapiens
325 CFTR 1080
Biochemical Activity Homo sapiens
326 VANGL1 81839
Proximity Label-MS Homo sapiens
327 VIM 7431
Affinity Capture-MS Homo sapiens
328 CEP170 9859
Affinity Capture-MS Homo sapiens
329 RPA3 6119
Proximity Label-MS Homo sapiens
330 CAV1 857
Affinity Capture-Western Homo sapiens
331 AIFM2 84883
Affinity Capture-MS Homo sapiens
332 C2orf88 84281
Affinity Capture-MS Homo sapiens
333 SIK1  
Biochemical Activity Homo sapiens
334 USP20  
Biochemical Activity Homo sapiens
335 VDAC3 7419
Affinity Capture-MS Homo sapiens
336 CC2D1A 54862
Proximity Label-MS Homo sapiens
337 UNC119B 84747
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PRKACA is involved
PathwayEvidenceSource
Activation of NMDA receptors and postsynaptic events IEA Reactome
Activation of NMDA receptors and postsynaptic events TAS Reactome
Adaptive Immune System TAS Reactome
ADORA2B mediated anti-inflammatory cytokines production IEA Reactome
ADORA2B mediated anti-inflammatory cytokines production TAS Reactome
Anchoring of the basal body to the plasma membrane TAS Reactome
Anti-inflammatory response favouring Leishmania parasite infection IEA Reactome
Anti-inflammatory response favouring Leishmania parasite infection TAS Reactome
Aquaporin-mediated transport TAS Reactome
AURKA Activation by TPX2 TAS Reactome
Axon guidance IEA Reactome
Axon guidance TAS Reactome
C-type lectin receptors (CLRs) TAS Reactome
Ca-dependent events IEA Reactome
Ca-dependent events TAS Reactome
Calmodulin induced events IEA Reactome
Calmodulin induced events TAS Reactome
CaM pathway IEA Reactome
CaM pathway TAS Reactome
Cardiac conduction TAS Reactome
CD209 (DC-SIGN) signaling TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
Centrosome maturation TAS Reactome
Cilium Assembly TAS Reactome
CREB1 phosphorylation through the activation of Adenylate Cyclase IEA Reactome
CREB1 phosphorylation through the activation of Adenylate Cyclase TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
DAG and IP3 signaling IEA Reactome
DAG and IP3 signaling TAS Reactome
DARPP-32 events TAS Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Degradation of GLI2 by the proteasome IEA Reactome
Developmental Biology IEA Reactome
Developmental Biology TAS Reactome
Disease IEA Reactome
Disease TAS Reactome
Disorders of Developmental Biology IEA Reactome
Disorders of Nervous System Development IEA Reactome
Factors involved in megakaryocyte development and platelet production TAS Reactome
FCGR3A-mediated IL10 synthesis IEA Reactome
FCGR3A-mediated IL10 synthesis TAS Reactome
G alpha (i) signalling events IEA Reactome
G alpha (i) signalling events TAS Reactome
G alpha (s) signalling events IEA Reactome
G alpha (s) signalling events TAS Reactome
G-protein mediated events IEA Reactome
G-protein mediated events TAS Reactome
G2/M Transition TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome TAS Reactome
Glucagon signaling in metabolic regulation TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion TAS Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
GPCR downstream signalling IEA Reactome
GPCR downstream signalling TAS Reactome
GPER1 signaling IEA Reactome
GPER1 signaling TAS Reactome
HDL assembly TAS Reactome
Hedgehog 'off' state TAS Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis TAS Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells TAS Reactome
Immune System TAS Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Integration of energy metabolism TAS Reactome
Integration of energy metabolism IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling TAS Reactome
Intracellular signaling by second messengers IEA Reactome
Intracellular signaling by second messengers TAS Reactome
Ion homeostasis TAS Reactome
Leishmania infection IEA Reactome
Leishmania infection TAS Reactome
Leishmania parasite growth and survival IEA Reactome
Leishmania parasite growth and survival TAS Reactome
Loss of function of MECP2 in Rett syndrome IEA Reactome
Loss of Nlp from mitotic centrosomes TAS Reactome
Loss of phosphorylation of MECP2 at T308 IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome TAS Reactome
M Phase TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK6/MAPK4 signaling TAS Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome
Metabolism of lipids IEA Reactome
Metabolism of proteins TAS Reactome
Mitochondrial protein degradation TAS Reactome
Mitotic G2-G2/M phases TAS Reactome
Mitotic Prometaphase TAS Reactome
Muscle contraction TAS Reactome
Nervous system development IEA Reactome
Nervous system development TAS Reactome
Neuronal System IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Opioid Signalling IEA Reactome
Opioid Signalling TAS Reactome
Organelle biogenesis and maintenance TAS Reactome
Parasitic Infection Pathways IEA Reactome
Parasitic Infection Pathways TAS Reactome
Pervasive developmental disorders IEA Reactome
PKA activation TAS Reactome
PKA activation in glucagon signalling TAS Reactome
PKA-mediated phosphorylation of CREB IEA Reactome
PKA-mediated phosphorylation of CREB TAS Reactome
PKA-mediated phosphorylation of key metabolic factors IEA Reactome
Plasma lipoprotein assembly TAS Reactome
Plasma lipoprotein assembly, remodeling, and clearance TAS Reactome
PLC beta mediated events IEA Reactome
PLC beta mediated events TAS Reactome
Post NMDA receptor activation events IEA Reactome
Post NMDA receptor activation events TAS Reactome
Rap1 signalling TAS Reactome
Recruitment of mitotic centrosome proteins and complexes TAS Reactome
Recruitment of NuMA to mitotic centrosomes TAS Reactome
Regulation of glycolysis by fructose 2,6-bisphosphate metabolism IEA Reactome
Regulation of insulin secretion IEA Reactome
Regulation of insulin secretion TAS Reactome
Regulation of MECP2 expression and activity IEA Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Response of endothelial cells to shear stress TAS Reactome
RET signaling TAS Reactome
RNA Polymerase II Transcription IEA Reactome
ROBO receptors bind AKAP5 IEA Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Hedgehog TAS Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Interleukins TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by ROBO receptors IEA Reactome
Signaling by VEGF TAS Reactome
Transcriptional Regulation by MECP2 IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transmission across Chemical Synapses TAS Reactome
Transport of small molecules TAS Reactome
Triglyceride catabolism IEA Reactome
Triglyceride metabolism IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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